Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing

Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing

Abstract Background Both RNA-Seq and sample freeze-thaw are ubiquitous. However, knowledge about the impact of freeze-thaw on downstream analyses is limited. The lack of common quality metrics that are sufficiently sensitive to freeze-thaw and RNA degradation, e.g. the RNA Integrity Score, makes suc...

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Bibliographic Details
Main Authors: Benjamin P. Kellman, Hratch M. Baghdassarian, Tiziano Pramparo, Isaac Shamie, Vahid Gazestani, Arjana Begzati, Shangzhong Li, Srinivasa Nalabolu, Sarah Murray, Linda Lopez, Karen Pierce, Eric Courchesne, Nathan E. Lewis
Format: Article
Language:English
Published: BMC 2021-01-01
Series:BMC Genomics
Subjects:
RNA-Seq
Quality control
Freeze-thaw
Sample preparation
Differential expression
Online Access:https://doi.org/10.1186/s12864-021-07381-z

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