The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite
Acquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case of HGT revealed by sequencing the plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP Q 202. These plastomes pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2016-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160249 |
| _version_ | 1818412816117268480 |
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| author | Tatiana Yurchenko Tereza Ševčíková Hynek Strnad Anzhelika Butenko Marek Eliáš |
| author_facet | Tatiana Yurchenko Tereza Ševčíková Hynek Strnad Anzhelika Butenko Marek Eliáš |
| author_sort | Tatiana Yurchenko |
| collection | DOAJ |
| description | Acquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case of HGT revealed by sequencing the plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP Q 202. These plastomes proved to harbour a unique cluster of six genes, most probably acquired from a bacterium of the phylum Bacteroidetes, with homologues in various bacteria, typically organized in a conserved uncharacterized putative operon. Sequence analyses of the six proteins encoded by the operon yielded the following annotation for them: (i) a novel family without discernible homologues; (ii) a new family within the superfamily of metallo-dependent hydrolases; (iii) a novel subgroup of the UbiA superfamily of prenyl transferases; (iv) a new clade within the sugar phosphate cyclase superfamily; (v) a new family within the xylose isomerase-like superfamily; and (vi) a hydrolase for a phosphate moiety-containing substrate. We suggest that the operon encodes enzymes of a pathway synthesizing an isoprenoid–cyclitol-derived compound, possibly an antimicrobial or other protective substance. To the best of our knowledge, this is the first report of an expansion of the metabolic capacity of a plastid mediated by HGT into the plastid genome. |
| first_indexed | 2024-12-14T10:53:19Z |
| format | Article |
| id | doaj.art-40632d0b9f204285bd661e2ca7a106b7 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-14T10:53:19Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-40632d0b9f204285bd661e2ca7a106b72022-12-21T23:05:05ZengThe Royal SocietyOpen Biology2046-24412016-01-0161110.1098/rsob.160249160249The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metaboliteTatiana YurchenkoTereza ŠevčíkováHynek StrnadAnzhelika ButenkoMarek EliášAcquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case of HGT revealed by sequencing the plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP Q 202. These plastomes proved to harbour a unique cluster of six genes, most probably acquired from a bacterium of the phylum Bacteroidetes, with homologues in various bacteria, typically organized in a conserved uncharacterized putative operon. Sequence analyses of the six proteins encoded by the operon yielded the following annotation for them: (i) a novel family without discernible homologues; (ii) a new family within the superfamily of metallo-dependent hydrolases; (iii) a novel subgroup of the UbiA superfamily of prenyl transferases; (iv) a new clade within the sugar phosphate cyclase superfamily; (v) a new family within the xylose isomerase-like superfamily; and (vi) a hydrolase for a phosphate moiety-containing substrate. We suggest that the operon encodes enzymes of a pathway synthesizing an isoprenoid–cyclitol-derived compound, possibly an antimicrobial or other protective substance. To the best of our knowledge, this is the first report of an expansion of the metabolic capacity of a plastid mediated by HGT into the plastid genome.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160249eustigmatophyceaehorizontal gene transferplastid genomesecondary metabolismsugar phosphate cyclase superfamilyubia superfamily |
| spellingShingle | Tatiana Yurchenko Tereza Ševčíková Hynek Strnad Anzhelika Butenko Marek Eliáš The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite Open Biology eustigmatophyceae horizontal gene transfer plastid genome secondary metabolism sugar phosphate cyclase superfamily ubia superfamily |
| title | The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite |
| title_full | The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite |
| title_fullStr | The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite |
| title_full_unstemmed | The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite |
| title_short | The plastid genome of some eustigmatophyte algae harbours a bacteria-derived six-gene cluster for biosynthesis of a novel secondary metabolite |
| title_sort | plastid genome of some eustigmatophyte algae harbours a bacteria derived six gene cluster for biosynthesis of a novel secondary metabolite |
| topic | eustigmatophyceae horizontal gene transfer plastid genome secondary metabolism sugar phosphate cyclase superfamily ubia superfamily |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160249 |
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