Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses
Nutrients lacking diet is responsible for major health problems like obesity, heart diseases, cancer, diabetes and inflammation. Microbial nutraceuticals can be the best alternative to resolve the drawbacks related to the phytochemical-based production of nutraceuticals. In vivo study was designed u...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364724000569 |
| _version_ | 1827284836027465728 |
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| author | Allah Nawaz Khan Shakira Ghazanfar Muhammad Nadeem Hassan Ajaz Ahmad Naeem Khan Sharjeel Khalid Humaira Yasmin |
| author_facet | Allah Nawaz Khan Shakira Ghazanfar Muhammad Nadeem Hassan Ajaz Ahmad Naeem Khan Sharjeel Khalid Humaira Yasmin |
| author_sort | Allah Nawaz Khan |
| collection | DOAJ |
| description | Nutrients lacking diet is responsible for major health problems like obesity, heart diseases, cancer, diabetes and inflammation. Microbial nutraceuticals can be the best alternative to resolve the drawbacks related to the phytochemical-based production of nutraceuticals. In vivo study was designed using mice to assess the anti-diabetic, antioxidant and anti-inflammatory potential of Lactobacillus agilis (L. agilis). Mice were put in control, toxic, standard and L. agilis dose-treated groups. The diabetics, oxidative stress and inflammation (in paw) were induced in mice. In alloxan-treated mice, the blood sugar level was elevated to 600 mg/dL and then it was decreased to 190 mg/dL after the L. agilis dose. Weight gain was increased from 51.48% to 68.56% in groups of diabetic and oxidatively stressed mice respectively as compared to the probiotic (L. agilis dose) group (25.99%) and standard drug-treated mice group (29.35%). The effect of L. agilis dose treatment on the alloxan-induced liver injury presented normal histology of hepatic cells with a well-preserved nucleus, cytoplasm, and hepatocytes in L. agilis dose group of mice. Antioxidant enzymes in L. agilis treated mice group were significantly improved as compared to alloxan treated mice with values 22.1 ± 0.18 μg/mg (super oxide dismutase), 8.9 ± 0.12 μg/mg (catalase), 4.1 ± 0.12 μg/mg (glutathione) and 20.8 ± 0.4 μg/mg (SOD), 7.2 ± 0.1 μg/mg (CAT), 3.7 ± 0.14 μg/mg (GSH) respectively. Paw size (thickness) of the treated mice was significantly reduced at T4 in mice group (L. agilis 1 mL dose) with value 2.1 ± 0.12 cm. Current in vivo study presented probiotic potential of L. agilis that can be used as nutraceutical. |
| first_indexed | 2024-04-24T10:05:07Z |
| format | Article |
| id | doaj.art-40636659f5134d6aabc163117eb3ad33 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-24T10:05:07Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-40636659f5134d6aabc163117eb3ad332024-04-13T04:20:57ZengElsevierJournal of King Saud University: Science1018-36472024-05-01365103144Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stressesAllah Nawaz Khan0Shakira Ghazanfar1Muhammad Nadeem Hassan2Ajaz Ahmad3Naeem Khan4Sharjeel Khalid5Humaira Yasmin6Department of Biosciences, COMSATS University Islamabad, Park Road, Islamabad, Pakistan; Health Services Academy (HSA) Park Road, Chak Shahzad, Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology NIGAB, National Agricultural Research Center NARC, Park Road, Islamabad 45500, PakistanDepartment of Biosciences, COMSATS University Islamabad, Park Road, Islamabad, PakistanDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaSenior Biological Scientist, Department of Agronomy, University of Florida, USANational Institute for Genomics and Advanced Biotechnology NIGAB, National Agricultural Research Center NARC, Park Road, Islamabad 45500, PakistanDepartment of Biosciences, COMSATS University Islamabad, Park Road, Islamabad, Pakistan; Corresponding author.Nutrients lacking diet is responsible for major health problems like obesity, heart diseases, cancer, diabetes and inflammation. Microbial nutraceuticals can be the best alternative to resolve the drawbacks related to the phytochemical-based production of nutraceuticals. In vivo study was designed using mice to assess the anti-diabetic, antioxidant and anti-inflammatory potential of Lactobacillus agilis (L. agilis). Mice were put in control, toxic, standard and L. agilis dose-treated groups. The diabetics, oxidative stress and inflammation (in paw) were induced in mice. In alloxan-treated mice, the blood sugar level was elevated to 600 mg/dL and then it was decreased to 190 mg/dL after the L. agilis dose. Weight gain was increased from 51.48% to 68.56% in groups of diabetic and oxidatively stressed mice respectively as compared to the probiotic (L. agilis dose) group (25.99%) and standard drug-treated mice group (29.35%). The effect of L. agilis dose treatment on the alloxan-induced liver injury presented normal histology of hepatic cells with a well-preserved nucleus, cytoplasm, and hepatocytes in L. agilis dose group of mice. Antioxidant enzymes in L. agilis treated mice group were significantly improved as compared to alloxan treated mice with values 22.1 ± 0.18 μg/mg (super oxide dismutase), 8.9 ± 0.12 μg/mg (catalase), 4.1 ± 0.12 μg/mg (glutathione) and 20.8 ± 0.4 μg/mg (SOD), 7.2 ± 0.1 μg/mg (CAT), 3.7 ± 0.14 μg/mg (GSH) respectively. Paw size (thickness) of the treated mice was significantly reduced at T4 in mice group (L. agilis 1 mL dose) with value 2.1 ± 0.12 cm. Current in vivo study presented probiotic potential of L. agilis that can be used as nutraceutical.http://www.sciencedirect.com/science/article/pii/S1018364724000569Lactobacillus agilisMouse ModulesStressesProbiotic |
| spellingShingle | Allah Nawaz Khan Shakira Ghazanfar Muhammad Nadeem Hassan Ajaz Ahmad Naeem Khan Sharjeel Khalid Humaira Yasmin Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses Journal of King Saud University: Science Lactobacillus agilis Mouse Modules Stresses Probiotic |
| title | Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses |
| title_full | Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses |
| title_fullStr | Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses |
| title_full_unstemmed | Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses |
| title_short | Probiotic potential of Lactobacillus agilis against oxidative, inflammatory and diabetic stresses |
| title_sort | probiotic potential of lactobacillus agilis against oxidative inflammatory and diabetic stresses |
| topic | Lactobacillus agilis Mouse Modules Stresses Probiotic |
| url | http://www.sciencedirect.com/science/article/pii/S1018364724000569 |
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