Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent
A spectrophotometric- reverse flow injection analysis (rFIA) method has been proposed for the determination of Nitrazepam (NIT) in pure and pharmaceutical preparations. The method is based upon the coupling reaction of NIT with a new reagent O-Coumaric acid (OCA) in the presence of sodium periodat...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2020-03-01
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| Series: | Baghdad Science Journal |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3133 |
| _version_ | 1828219896916344832 |
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| author | Sadeem Subhi Abed |
| author_facet | Sadeem Subhi Abed |
| author_sort | Sadeem Subhi Abed |
| collection | DOAJ |
| description | A spectrophotometric- reverse flow injection analysis (rFIA) method has been proposed for the determination of Nitrazepam (NIT) in pure and pharmaceutical preparations. The method is based upon the coupling reaction of NIT with a new reagent O-Coumaric acid (OCA) in the presence of sodium periodate in an aqueous solution. The blue color product was measured at 632 nm. The variation (chemical and physical parameters) related with reverse flow system were estimated. The linearity was over the range 15 - 450 µg/mL of NIT with detection limits and limit of quantification of 3.425 and 11.417 µg mL-1 NIT,respectively. The sample throughput of 28 samples per hour was achieved. Suggested method was successfully applied for the determination of NIT in its dosage forms. |
| first_indexed | 2024-04-12T16:20:47Z |
| format | Article |
| id | doaj.art-4064f523303742ed9b274663c20cc053 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-04-12T16:20:47Z |
| publishDate | 2020-03-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-4064f523303742ed9b274663c20cc0532022-12-22T03:25:33ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862020-03-01171(Suppl.)10.21123/bsj.2020.17.1(Suppl.).0265Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic ReagentSadeem Subhi Abed0University of Baghdad,college of Science, deparment of ChemistryA spectrophotometric- reverse flow injection analysis (rFIA) method has been proposed for the determination of Nitrazepam (NIT) in pure and pharmaceutical preparations. The method is based upon the coupling reaction of NIT with a new reagent O-Coumaric acid (OCA) in the presence of sodium periodate in an aqueous solution. The blue color product was measured at 632 nm. The variation (chemical and physical parameters) related with reverse flow system were estimated. The linearity was over the range 15 - 450 µg/mL of NIT with detection limits and limit of quantification of 3.425 and 11.417 µg mL-1 NIT,respectively. The sample throughput of 28 samples per hour was achieved. Suggested method was successfully applied for the determination of NIT in its dosage forms.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3133Chromogenic reagent, Nitrazepam, O- Coumaric acid, Reverse flow injection method |
| spellingShingle | Sadeem Subhi Abed Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent Baghdad Science Journal Chromogenic reagent, Nitrazepam, O- Coumaric acid, Reverse flow injection method |
| title | Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent |
| title_full | Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent |
| title_fullStr | Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent |
| title_full_unstemmed | Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent |
| title_short | Spectrophotometric and Reverse Flow Injection Method Determination of Nitrazepam in Pharmaceuticals Using O-Coumaric Acid as a New Chromogenic Reagent |
| title_sort | spectrophotometric and reverse flow injection method determination of nitrazepam in pharmaceuticals using o coumaric acid as a new chromogenic reagent |
| topic | Chromogenic reagent, Nitrazepam, O- Coumaric acid, Reverse flow injection method |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3133 |
| work_keys_str_mv | AT sadeemsubhiabed spectrophotometricandreverseflowinjectionmethoddeterminationofnitrazepaminpharmaceuticalsusingocoumaricacidasanewchromogenicreagent |