In vitro regeneration of Pistacia vera L. from nodal explants
To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent re...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-05-01
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| Series: | Journal of Forest Science |
| Subjects: | |
| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-201605-0002_in-vitro-regeneration-of-pistacia-vera-l-from-nodal-explants.php |
| _version_ | 1797898763152392192 |
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| author | B. Benmahioul M. Kaïd-Harche F. Daguin |
| author_facet | B. Benmahioul M. Kaïd-Harche F. Daguin |
| author_sort | B. Benmahioul |
| collection | DOAJ |
| description | To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%. |
| first_indexed | 2024-04-10T08:18:17Z |
| format | Article |
| id | doaj.art-406a680b5b784f7d936768618326eb57 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-10T08:18:17Z |
| publishDate | 2016-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-406a680b5b784f7d936768618326eb572023-02-23T03:42:44ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2016-05-0162519820310.17221/82/2015-JFSjfs-201605-0002In vitro regeneration of Pistacia vera L. from nodal explantsB. Benmahioul0M. Kaïd-Harche1F. Daguin2Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Abou-Bekr Belkaïd Tlemcen, Tlemcen, AlgeriaDepartment of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, Oran, AlgeriaResearch Institute of Horticulture and Seeds, University of Angers, Angers, FranceTo enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.https://jfs.agriculturejournals.cz/artkey/jfs-201605-0002_in-vitro-regeneration-of-pistacia-vera-l-from-nodal-explants.phpsterilizing agentsmorphogenic responsecytokininshoot proliferationex vitro rootingtissue culture |
| spellingShingle | B. Benmahioul M. Kaïd-Harche F. Daguin In vitro regeneration of Pistacia vera L. from nodal explants Journal of Forest Science sterilizing agents morphogenic response cytokinin shoot proliferation ex vitro rooting tissue culture |
| title | In vitro regeneration of Pistacia vera L. from nodal explants |
| title_full | In vitro regeneration of Pistacia vera L. from nodal explants |
| title_fullStr | In vitro regeneration of Pistacia vera L. from nodal explants |
| title_full_unstemmed | In vitro regeneration of Pistacia vera L. from nodal explants |
| title_short | In vitro regeneration of Pistacia vera L. from nodal explants |
| title_sort | in vitro regeneration of pistacia vera l from nodal explants |
| topic | sterilizing agents morphogenic response cytokinin shoot proliferation ex vitro rooting tissue culture |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-201605-0002_in-vitro-regeneration-of-pistacia-vera-l-from-nodal-explants.php |
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