Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model
Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistanc...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-01-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-7737/7/1/8 |
| _version_ | 1827843348953563136 |
|---|---|
| author | Hidetomo Iwano Yusuke Inoue Takuji Takasago Hironori Kobayashi Takaaki Furusawa Kotomi Taniguchi Jumpei Fujiki Hiroshi Yokota Masaru Usui Yasunori Tanji Katsuro Hagiwara Hidetoshi Higuchi Yutaka Tamura |
| author_facet | Hidetomo Iwano Yusuke Inoue Takuji Takasago Hironori Kobayashi Takaaki Furusawa Kotomi Taniguchi Jumpei Fujiki Hiroshi Yokota Masaru Usui Yasunori Tanji Katsuro Hagiwara Hidetoshi Higuchi Yutaka Tamura |
| author_sort | Hidetomo Iwano |
| collection | DOAJ |
| description | Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S. aureus phages ΦSA012 and ΦSA039 against S. aureus strains isolated from mastitic cows. These phages could kill all S. aureus (93 strains from 40 genotypes) and methicillin-resistant S. aureus (six strains from six genotypes) strains tested. Using a mouse mastitis model, we demonstrated that ΦSA012 reduced proliferation of S. aureus and inflammation in the mammary gland. Furthermore, intravenous or intraperitoneal phage administration reduced proliferation of S. aureus in the mammary glands. These results suggest that broad host range phages ΦSA012 is potential antibacterial agents for dairy production medicine. |
| first_indexed | 2024-03-12T08:24:29Z |
| format | Article |
| id | doaj.art-406f446bf0d742c4a3e2ede2257790f2 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T08:24:29Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-406f446bf0d742c4a3e2ede2257790f22023-09-02T18:10:03ZengMDPI AGBiology2079-77372018-01-0171810.3390/biology7010008biology7010008Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis ModelHidetomo Iwano0Yusuke Inoue1Takuji Takasago2Hironori Kobayashi3Takaaki Furusawa4Kotomi Taniguchi5Jumpei Fujiki6Hiroshi Yokota7Masaru Usui8Yasunori Tanji9Katsuro Hagiwara10Hidetoshi Higuchi11Yutaka Tamura12Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanDepartment of Bioengineering, Tokyo Institute of Technology, Yokohama 226-8502, JapanLaboratory of Veterinary Virology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Veterinary Hygiene, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanLaboratory of Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanBovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S. aureus phages ΦSA012 and ΦSA039 against S. aureus strains isolated from mastitic cows. These phages could kill all S. aureus (93 strains from 40 genotypes) and methicillin-resistant S. aureus (six strains from six genotypes) strains tested. Using a mouse mastitis model, we demonstrated that ΦSA012 reduced proliferation of S. aureus and inflammation in the mammary gland. Furthermore, intravenous or intraperitoneal phage administration reduced proliferation of S. aureus in the mammary glands. These results suggest that broad host range phages ΦSA012 is potential antibacterial agents for dairy production medicine.http://www.mdpi.com/2079-7737/7/1/8bacteriophagemastitisStaphylococcus aureus |
| spellingShingle | Hidetomo Iwano Yusuke Inoue Takuji Takasago Hironori Kobayashi Takaaki Furusawa Kotomi Taniguchi Jumpei Fujiki Hiroshi Yokota Masaru Usui Yasunori Tanji Katsuro Hagiwara Hidetoshi Higuchi Yutaka Tamura Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model Biology bacteriophage mastitis Staphylococcus aureus |
| title | Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model |
| title_full | Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model |
| title_fullStr | Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model |
| title_full_unstemmed | Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model |
| title_short | Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model |
| title_sort | bacteriophage φsa012 has a broad host range against staphylococcus aureus and effective lytic capacity in a mouse mastitis model |
| topic | bacteriophage mastitis Staphylococcus aureus |
| url | http://www.mdpi.com/2079-7737/7/1/8 |
| work_keys_str_mv | AT hidetomoiwano bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT yusukeinoue bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT takujitakasago bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT hironorikobayashi bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT takaakifurusawa bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT kotomitaniguchi bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT jumpeifujiki bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT hiroshiyokota bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT masaruusui bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT yasunoritanji bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT katsurohagiwara bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT hidetoshihiguchi bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel AT yutakatamura bacteriophagephsa012hasabroadhostrangeagainststaphylococcusaureusandeffectivelyticcapacityinamousemastitismodel |