Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects
Septoria leaf blotch is a foliar wheat disease controlled by a combination of plant genetic resistances and fungicides use. R-gene-based qualitative resistance durability is limited due to gene-for-gene interactions with fungal avirulence (Avr) genes. Quantitative resistance is considered more durab...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1128546/full |
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| author | Camilla Langlands-Perry Camilla Langlands-Perry Anaïs Pitarch Nicolas Lapalu Murielle Cuenin Christophe Bergez Alicia Noly Reda Amezrou Sandrine Gélisse Célia Barrachina Hugues Parrinello Frédéric Suffert Romain Valade Thierry C. Marcel |
| author_facet | Camilla Langlands-Perry Camilla Langlands-Perry Anaïs Pitarch Nicolas Lapalu Murielle Cuenin Christophe Bergez Alicia Noly Reda Amezrou Sandrine Gélisse Célia Barrachina Hugues Parrinello Frédéric Suffert Romain Valade Thierry C. Marcel |
| author_sort | Camilla Langlands-Perry |
| collection | DOAJ |
| description | Septoria leaf blotch is a foliar wheat disease controlled by a combination of plant genetic resistances and fungicides use. R-gene-based qualitative resistance durability is limited due to gene-for-gene interactions with fungal avirulence (Avr) genes. Quantitative resistance is considered more durable but the mechanisms involved are not well documented. We hypothesize that genes involved in quantitative and qualitative plant-pathogen interactions are similar. A bi-parental population of Zymoseptoria tritici was inoculated on wheat cultivar ‘Renan’ and a linkage analysis performed to map QTL. Three pathogenicity QTL, Qzt-I05-1, Qzt-I05-6 and Qzt-I07-13, were mapped on chromosomes 1, 6 and 13 in Z. tritici, and a candidate pathogenicity gene on chromosome 6 was selected based on its effector-like characteristics. The candidate gene was cloned by Agrobacterium tumefaciens-mediated transformation, and a pathology test assessed the effect of the mutant strains on ‘Renan’. This gene was demonstrated to be involved in quantitative pathogenicity. By cloning a newly annotated quantitative-effect gene in Z. tritici that is effector-like, we demonstrated that genes underlying pathogenicity QTL can be similar to Avr genes. This opens up the previously probed possibility that ‘gene-for-gene’ underlies not only qualitative but also quantitative plant-pathogen interactions in this pathosystem. |
| first_indexed | 2024-04-09T13:28:24Z |
| format | Article |
| id | doaj.art-407096198f314f27ab81c3e6a61a1776 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:28:24Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-407096198f314f27ab81c3e6a61a17762023-05-10T05:21:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11285461128546Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effectsCamilla Langlands-Perry0Camilla Langlands-Perry1Anaïs Pitarch2Nicolas Lapalu3Murielle Cuenin4Christophe Bergez5Alicia Noly6Reda Amezrou7Sandrine Gélisse8Célia Barrachina9Hugues Parrinello10Frédéric Suffert11Romain Valade12Thierry C. Marcel13Université Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceARVALIS Institut du Végétal, Boigneville, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceMGX-Montpellier GenomiX, Univ. Montpellier, CNRS, INSERM, Montpellier, FranceMGX-Montpellier GenomiX, Univ. Montpellier, CNRS, INSERM, Montpellier, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceARVALIS Institut du Végétal, Boigneville, FranceUniversité Paris-Saclay, INRAE, UR BIOGER, Palaiseau, FranceSeptoria leaf blotch is a foliar wheat disease controlled by a combination of plant genetic resistances and fungicides use. R-gene-based qualitative resistance durability is limited due to gene-for-gene interactions with fungal avirulence (Avr) genes. Quantitative resistance is considered more durable but the mechanisms involved are not well documented. We hypothesize that genes involved in quantitative and qualitative plant-pathogen interactions are similar. A bi-parental population of Zymoseptoria tritici was inoculated on wheat cultivar ‘Renan’ and a linkage analysis performed to map QTL. Three pathogenicity QTL, Qzt-I05-1, Qzt-I05-6 and Qzt-I07-13, were mapped on chromosomes 1, 6 and 13 in Z. tritici, and a candidate pathogenicity gene on chromosome 6 was selected based on its effector-like characteristics. The candidate gene was cloned by Agrobacterium tumefaciens-mediated transformation, and a pathology test assessed the effect of the mutant strains on ‘Renan’. This gene was demonstrated to be involved in quantitative pathogenicity. By cloning a newly annotated quantitative-effect gene in Z. tritici that is effector-like, we demonstrated that genes underlying pathogenicity QTL can be similar to Avr genes. This opens up the previously probed possibility that ‘gene-for-gene’ underlies not only qualitative but also quantitative plant-pathogen interactions in this pathosystem.https://www.frontiersin.org/articles/10.3389/fpls.2023.1128546/fullquantitative pathogenicityquantitative trait locismall secreted proteinsSeptoria tritici blotch (STB)Triticum aestivum (L.) |
| spellingShingle | Camilla Langlands-Perry Camilla Langlands-Perry Anaïs Pitarch Nicolas Lapalu Murielle Cuenin Christophe Bergez Alicia Noly Reda Amezrou Sandrine Gélisse Célia Barrachina Hugues Parrinello Frédéric Suffert Romain Valade Thierry C. Marcel Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects Frontiers in Plant Science quantitative pathogenicity quantitative trait loci small secreted proteins Septoria tritici blotch (STB) Triticum aestivum (L.) |
| title | Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| title_full | Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| title_fullStr | Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| title_full_unstemmed | Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| title_short | Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| title_sort | quantitative and qualitative plant pathogen interactions call upon similar pathogenicity genes with a spectrum of effects |
| topic | quantitative pathogenicity quantitative trait loci small secreted proteins Septoria tritici blotch (STB) Triticum aestivum (L.) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1128546/full |
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