The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease
Drechslera graminea is the causal agent of leaf-stripe disease in barley leading to substantial crop losses. The current work aimed to investigate the antagonistic activity of Trichoderma harzianum against D. graminea. Pathogen was isolated from the naturally infected barely leaves and molecularl...
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| Format: | Article |
| Language: | English |
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The National Information and Documentation Centre (NIDOC) , Egypt
2024-04-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_350283_8682b47ce049fc228603816349481e17.pdf |
| _version_ | 1827280159584026624 |
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| author | Sherin P. Mekhail Marwa S. Fouad |
| author_facet | Sherin P. Mekhail Marwa S. Fouad |
| author_sort | Sherin P. Mekhail |
| collection | DOAJ |
| description | Drechslera graminea is the causal agent of leaf-stripe disease in barley leading to
substantial crop losses. The current work aimed to investigate the antagonistic activity of
Trichoderma harzianum against D. graminea. Pathogen was isolated from the naturally
infected barely leaves and molecularly identified. The identified isolate was deposited in
GenBank with an accession number of OR827023. In vitro dual culture assay showed a
decrease in D. graminea radial growth with a growth inhibition percentage of 77.8 %. T.
harzianum culture filtrate (TCF) (30×106
spore/ ml) was efficiently applied in the greenhouse
causing a significant reduction in final disease severity (FDS %) by 77 %, compared to the
positive control. Moreover, application of TCF against the natural infection in the open field
reduced FDS, the area under the disease progress curve (AUDPC), and the average coefficient
of infection (ACI). Furthermore, TCF significantly improved yield components compared to
the untreated control. To assess the plant response on a molecular basis, the protein profile was
analyzed showing a wide range of variability. Five polymorphic bands were recognized;
mainly 66, 48, 35, 29, and 16 kDa. The band with a molecular weight of 66 kDa had
completely disappeared in the negative control, but was observed in the positive control and
upon application of TCF. Meanwhile, the bands with molecular weights of 35, 29, and 16 kDa
were recorded only in the positive control but were completely vanished in the negative
control and upon application of TCF. Additionally, upon application of TCF under the
infection stress, phenylalanine ammonia lyase (PAL) mRNA transcript accumulation had
increased significantly in a way that exceeded two-fold of the non-infected control. Finally, it
was concluded that T. harzianum was able to reduce leaf-stripe infection damage and
promoted the barley-induced resistance in the susceptible cultivars; with the improvement of
vegetative growth parameters, which may be a real prospect for leaf-stripe disease
management in the future. |
| first_indexed | 2024-04-24T08:31:05Z |
| format | Article |
| id | doaj.art-4079971338e64fa5a3aa163310c2a089 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:31:05Z |
| publishDate | 2024-04-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-4079971338e64fa5a3aa163310c2a0892024-04-16T19:25:38ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942024-04-01822393241310.21608/NRMJ.2024.277435.1498The antagonistic potential of Trichoderma harzianum against barley leaf-stripe diseaseSherin P. Mekhail0Marwa S. Fouad1Barley disease Research Department, Plant Pathology Research Institute, Agricultural Research center, Giza 12619, EgyptSeed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research center, Giza 12619, EgyptDrechslera graminea is the causal agent of leaf-stripe disease in barley leading to substantial crop losses. The current work aimed to investigate the antagonistic activity of Trichoderma harzianum against D. graminea. Pathogen was isolated from the naturally infected barely leaves and molecularly identified. The identified isolate was deposited in GenBank with an accession number of OR827023. In vitro dual culture assay showed a decrease in D. graminea radial growth with a growth inhibition percentage of 77.8 %. T. harzianum culture filtrate (TCF) (30×106 spore/ ml) was efficiently applied in the greenhouse causing a significant reduction in final disease severity (FDS %) by 77 %, compared to the positive control. Moreover, application of TCF against the natural infection in the open field reduced FDS, the area under the disease progress curve (AUDPC), and the average coefficient of infection (ACI). Furthermore, TCF significantly improved yield components compared to the untreated control. To assess the plant response on a molecular basis, the protein profile was analyzed showing a wide range of variability. Five polymorphic bands were recognized; mainly 66, 48, 35, 29, and 16 kDa. The band with a molecular weight of 66 kDa had completely disappeared in the negative control, but was observed in the positive control and upon application of TCF. Meanwhile, the bands with molecular weights of 35, 29, and 16 kDa were recorded only in the positive control but were completely vanished in the negative control and upon application of TCF. Additionally, upon application of TCF under the infection stress, phenylalanine ammonia lyase (PAL) mRNA transcript accumulation had increased significantly in a way that exceeded two-fold of the non-infected control. Finally, it was concluded that T. harzianum was able to reduce leaf-stripe infection damage and promoted the barley-induced resistance in the susceptible cultivars; with the improvement of vegetative growth parameters, which may be a real prospect for leaf-stripe disease management in the future.https://nrmj.journals.ekb.eg/article_350283_8682b47ce049fc228603816349481e17.pdfdrechslera gramineabioagentsds-pagepal transcriptsbarely |
| spellingShingle | Sherin P. Mekhail Marwa S. Fouad The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease Novel Research in Microbiology Journal drechslera graminea bioagent sds-page pal transcripts barely |
| title | The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease |
| title_full | The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease |
| title_fullStr | The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease |
| title_full_unstemmed | The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease |
| title_short | The antagonistic potential of Trichoderma harzianum against barley leaf-stripe disease |
| title_sort | antagonistic potential of trichoderma harzianum against barley leaf stripe disease |
| topic | drechslera graminea bioagent sds-page pal transcripts barely |
| url | https://nrmj.journals.ekb.eg/article_350283_8682b47ce049fc228603816349481e17.pdf |
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