Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma
Lian He,1 Yusai Xie,2 Yusong Qiu,1 Yong Zhang1 1Department of Pathology, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning, 110042, People’s Republic of China; 2Laboratory of Basic Medicine, General Hospital of Northern Theatre Command,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-08-01
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| Series: | Journal of Hepatocellular Carcinoma |
| Subjects: | |
| Online Access: | https://www.dovepress.com/pan-cancer-profiling-and-digital-pathology-analysis-reveal-negative-pr-peer-reviewed-fulltext-article-JHC |
| _version_ | 1797745461916860416 |
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| author | He L Xie Y Qiu Y Zhang Y |
| author_facet | He L Xie Y Qiu Y Zhang Y |
| author_sort | He L |
| collection | DOAJ |
| description | Lian He,1 Yusai Xie,2 Yusong Qiu,1 Yong Zhang1 1Department of Pathology, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning, 110042, People’s Republic of China; 2Laboratory of Basic Medicine, General Hospital of Northern Theatre Command, Shenyang, Liaoning, 110016, People’s Republic of ChinaCorrespondence: Yusai Xie, Laboratory of Basic Medicine, General Hospital of Northern Theatre Command, Shenyang, Liaoning, 110016, People’s Republic of China, Email ysxie@cmu.edu.cn Yong Zhang, Department of Pathology, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning, 110042, People’s Republic of China, Email zhycmu@163.comPurpose: ZPR1 is a zinc finger-containing protein that plays a crucial role in neurodegenerative diseases, lipid metabolism disorders, and non-alcoholic fatty liver disease. However, the expression pattern, prognostic value, and treatment response of ZPR1 in pan-cancer and hepatocellular carcinoma (HCC) remain unclear.Patients and Methods: Pan-cancer expression profiles and relevant clinical data were acquired from UCSC Xena platform. Pan-cancer expression, epigenetic profile, and clinical correlation analysis for ZPR1 were performed. We next explored the prognostic significance and potential biological functions of ZPR1 in HCC. Furthermore, the relationship between ZPR1 and immune infiltration and treatment response was investigated. Finally, quantitative immunohistochemistry (IHC) analysis was applied to assess the correlation of ZPR1 expression and immune microenvironment in HCC tissues using Qupath software.Results: ZPR1 was differentially expressed in most tumor types and significantly up-regulated in HCC. ZPR1 showed hypo-methylated status in most tumors. Pan-cancer correlation analysis indicated that ZPR1 was closely associated with clinicopathological factors and TMB, MSI, and stemness index in HCC. High ZPR1 expression could be an independent risk factor for adverse prognosis in HCC. ZPR1 correlated with immune cell infiltration and therapeutic response. Finally, IHC results suggested that ZPR1 correlated with CD4, CD56, CD68, and PD-L1 expression and is a promising pathological diagnostic marker in HCC.Conclusion: Immune infiltrate-associated ZPR1 could be considered a novel negative prognostic biomarker for therapeutic response in HCC.Keywords: hepatocellular carcinoma, ZPR1, prognosis, digital pathology, pan-cancer |
| first_indexed | 2024-03-12T15:23:35Z |
| format | Article |
| id | doaj.art-407b7af600834946ab74156f5e01ec67 |
| institution | Directory Open Access Journal |
| issn | 2253-5969 |
| language | English |
| last_indexed | 2024-03-12T15:23:35Z |
| publishDate | 2023-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Hepatocellular Carcinoma |
| spelling | doaj.art-407b7af600834946ab74156f5e01ec672023-08-10T19:04:44ZengDove Medical PressJournal of Hepatocellular Carcinoma2253-59692023-08-01Volume 101309132585793Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular CarcinomaHe LXie YQiu YZhang YLian He,1 Yusai Xie,2 Yusong Qiu,1 Yong Zhang1 1Department of Pathology, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning, 110042, People’s Republic of China; 2Laboratory of Basic Medicine, General Hospital of Northern Theatre Command, Shenyang, Liaoning, 110016, People’s Republic of ChinaCorrespondence: Yusai Xie, Laboratory of Basic Medicine, General Hospital of Northern Theatre Command, Shenyang, Liaoning, 110016, People’s Republic of China, Email ysxie@cmu.edu.cn Yong Zhang, Department of Pathology, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning, 110042, People’s Republic of China, Email zhycmu@163.comPurpose: ZPR1 is a zinc finger-containing protein that plays a crucial role in neurodegenerative diseases, lipid metabolism disorders, and non-alcoholic fatty liver disease. However, the expression pattern, prognostic value, and treatment response of ZPR1 in pan-cancer and hepatocellular carcinoma (HCC) remain unclear.Patients and Methods: Pan-cancer expression profiles and relevant clinical data were acquired from UCSC Xena platform. Pan-cancer expression, epigenetic profile, and clinical correlation analysis for ZPR1 were performed. We next explored the prognostic significance and potential biological functions of ZPR1 in HCC. Furthermore, the relationship between ZPR1 and immune infiltration and treatment response was investigated. Finally, quantitative immunohistochemistry (IHC) analysis was applied to assess the correlation of ZPR1 expression and immune microenvironment in HCC tissues using Qupath software.Results: ZPR1 was differentially expressed in most tumor types and significantly up-regulated in HCC. ZPR1 showed hypo-methylated status in most tumors. Pan-cancer correlation analysis indicated that ZPR1 was closely associated with clinicopathological factors and TMB, MSI, and stemness index in HCC. High ZPR1 expression could be an independent risk factor for adverse prognosis in HCC. ZPR1 correlated with immune cell infiltration and therapeutic response. Finally, IHC results suggested that ZPR1 correlated with CD4, CD56, CD68, and PD-L1 expression and is a promising pathological diagnostic marker in HCC.Conclusion: Immune infiltrate-associated ZPR1 could be considered a novel negative prognostic biomarker for therapeutic response in HCC.Keywords: hepatocellular carcinoma, ZPR1, prognosis, digital pathology, pan-cancerhttps://www.dovepress.com/pan-cancer-profiling-and-digital-pathology-analysis-reveal-negative-pr-peer-reviewed-fulltext-article-JHChepatocellular carcinomazpr1prognosisdigital pathologypan-cancer |
| spellingShingle | He L Xie Y Qiu Y Zhang Y Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma Journal of Hepatocellular Carcinoma hepatocellular carcinoma zpr1 prognosis digital pathology pan-cancer |
| title | Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma |
| title_full | Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma |
| title_fullStr | Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma |
| title_full_unstemmed | Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma |
| title_short | Pan-Cancer Profiling and Digital Pathology Analysis Reveal Negative Prognostic Biomarker ZPR1 Associated with Immune Infiltration and Treatment Response in Hepatocellular Carcinoma |
| title_sort | pan cancer profiling and digital pathology analysis reveal negative prognostic biomarker zpr1 associated with immune infiltration and treatment response in hepatocellular carcinoma |
| topic | hepatocellular carcinoma zpr1 prognosis digital pathology pan-cancer |
| url | https://www.dovepress.com/pan-cancer-profiling-and-digital-pathology-analysis-reveal-negative-pr-peer-reviewed-fulltext-article-JHC |
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