Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy
Due to the low genetic diversity in the current wheat germplasm, gene mining from wild relatives is essential to develop new wheat cultivars that are more resilient to the changing climate. Aegilops tauschii, the D-genome donor of bread wheat, is a great gene source for wheat breeding; however, iden...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1270925/full |
| _version_ | 1797447840283230208 |
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| author | Monir Idres Yahya Ahmed Yasir Serag Alnor Gorafi Yasir Serag Alnor Gorafi Nasrein Mohamed Kamal Nasrein Mohamed Kamal Mohammed Yousif Balla Mohammed Yousif Balla Izzat Sidahmed Ali Tahir Izzat Sidahmed Ali Tahir Lipeng Zheng Naoto Kawakami Hisashi Tsujimoto |
| author_facet | Monir Idres Yahya Ahmed Yasir Serag Alnor Gorafi Yasir Serag Alnor Gorafi Nasrein Mohamed Kamal Nasrein Mohamed Kamal Mohammed Yousif Balla Mohammed Yousif Balla Izzat Sidahmed Ali Tahir Izzat Sidahmed Ali Tahir Lipeng Zheng Naoto Kawakami Hisashi Tsujimoto |
| author_sort | Monir Idres Yahya Ahmed |
| collection | DOAJ |
| description | Due to the low genetic diversity in the current wheat germplasm, gene mining from wild relatives is essential to develop new wheat cultivars that are more resilient to the changing climate. Aegilops tauschii, the D-genome donor of bread wheat, is a great gene source for wheat breeding; however, identifying suitable genes from Ae. tauschii is challenging due to the different morphology and the wide intra-specific variation within the species. In this study, we developed a platform for the systematic evaluation of Ae. tauschii traits in the background of the hexaploid wheat cultivar ‘Norin 61’ and thus for the identification of QTLs and genes. To validate our platform, we analyzed the seed dormancy trait that confers resistance to preharvest sprouting. We used a multiple synthetic derivative (MSD) population containing a genetic diversity of 43 Ae. tauschii accessions representing the full range of the species. Our results showed that only nine accessions in the population provided seed dormancy, and KU-2039 from Afghanistan had the highest level of seed dormancy. Therefore, 166 backcross inbred lines (BILs) were developed by crossing the synthetic wheat derived from KU-2039 with ‘Norin 61’ as the recurrent parent. The QTL mapping revealed one novel QTL, Qsd.alrc.5D, associated with dormancy explaining 41.7% of the phenotypic variation and other five unstable QTLs, two of which have already been reported. The Qsd.alrc.5D, identified for the first time within the natural variation of wheat, would be a valuable contribution to breeding after appropriate validation. The proposed platform that used the MSD population derived from the diverse Ae. tauschii gene pool and recombinant inbred lines proved to be a valuable platform for mining new and important QTLs or alleles, such as the novel seed dormancy QTL identified here. Likewise, such a platform harboring genetic diversity from wheat wild relatives could be a useful source for mining agronomically important traits, especially in the era of climate change and the narrow genetic diversity within the current wheat germplasm. |
| first_indexed | 2024-03-09T14:01:47Z |
| format | Article |
| id | doaj.art-407bfdc7e7da46c3a9360954e4d8c164 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-09T14:01:47Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-407bfdc7e7da46c3a9360954e4d8c1642023-11-30T07:22:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12709251270925Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancyMonir Idres Yahya Ahmed0Yasir Serag Alnor Gorafi1Yasir Serag Alnor Gorafi2Nasrein Mohamed Kamal3Nasrein Mohamed Kamal4Mohammed Yousif Balla5Mohammed Yousif Balla6Izzat Sidahmed Ali Tahir7Izzat Sidahmed Ali Tahir8Lipeng Zheng9Naoto Kawakami10Hisashi Tsujimoto11United Graduate School of Agricultural Sciences, Tottori University, Tottori, JapanInternational Platform for Dryland Research and Education, Tottori University, Tottori, JapanGezira Research Station, Agricultural Research Corporation (ARC), Wad-Medani, SudanGezira Research Station, Agricultural Research Corporation (ARC), Wad-Medani, SudanArid Land Research Center, Tottori University, Tottori, JapanGezira Research Station, Agricultural Research Corporation (ARC), Wad-Medani, SudanArid Land Research Center, Tottori University, Tottori, JapanGezira Research Station, Agricultural Research Corporation (ARC), Wad-Medani, SudanArid Land Research Center, Tottori University, Tottori, JapanDepartment of Life Sciences, School of Agriculture, Meiji University, Kawasaki, JapanDepartment of Life Sciences, School of Agriculture, Meiji University, Kawasaki, JapanArid Land Research Center, Tottori University, Tottori, JapanDue to the low genetic diversity in the current wheat germplasm, gene mining from wild relatives is essential to develop new wheat cultivars that are more resilient to the changing climate. Aegilops tauschii, the D-genome donor of bread wheat, is a great gene source for wheat breeding; however, identifying suitable genes from Ae. tauschii is challenging due to the different morphology and the wide intra-specific variation within the species. In this study, we developed a platform for the systematic evaluation of Ae. tauschii traits in the background of the hexaploid wheat cultivar ‘Norin 61’ and thus for the identification of QTLs and genes. To validate our platform, we analyzed the seed dormancy trait that confers resistance to preharvest sprouting. We used a multiple synthetic derivative (MSD) population containing a genetic diversity of 43 Ae. tauschii accessions representing the full range of the species. Our results showed that only nine accessions in the population provided seed dormancy, and KU-2039 from Afghanistan had the highest level of seed dormancy. Therefore, 166 backcross inbred lines (BILs) were developed by crossing the synthetic wheat derived from KU-2039 with ‘Norin 61’ as the recurrent parent. The QTL mapping revealed one novel QTL, Qsd.alrc.5D, associated with dormancy explaining 41.7% of the phenotypic variation and other five unstable QTLs, two of which have already been reported. The Qsd.alrc.5D, identified for the first time within the natural variation of wheat, would be a valuable contribution to breeding after appropriate validation. The proposed platform that used the MSD population derived from the diverse Ae. tauschii gene pool and recombinant inbred lines proved to be a valuable platform for mining new and important QTLs or alleles, such as the novel seed dormancy QTL identified here. Likewise, such a platform harboring genetic diversity from wheat wild relatives could be a useful source for mining agronomically important traits, especially in the era of climate change and the narrow genetic diversity within the current wheat germplasm.https://www.frontiersin.org/articles/10.3389/fpls.2023.1270925/fullbread wheatAegilops tauschiigene miningseed dormancyQTL analysisgermplasm enhancement |
| spellingShingle | Monir Idres Yahya Ahmed Yasir Serag Alnor Gorafi Yasir Serag Alnor Gorafi Nasrein Mohamed Kamal Nasrein Mohamed Kamal Mohammed Yousif Balla Mohammed Yousif Balla Izzat Sidahmed Ali Tahir Izzat Sidahmed Ali Tahir Lipeng Zheng Naoto Kawakami Hisashi Tsujimoto Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy Frontiers in Plant Science bread wheat Aegilops tauschii gene mining seed dormancy QTL analysis germplasm enhancement |
| title | Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy |
| title_full | Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy |
| title_fullStr | Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy |
| title_full_unstemmed | Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy |
| title_short | Mining Aegilops tauschii genetic diversity in the background of bread wheat revealed a novel QTL for seed dormancy |
| title_sort | mining aegilops tauschii genetic diversity in the background of bread wheat revealed a novel qtl for seed dormancy |
| topic | bread wheat Aegilops tauschii gene mining seed dormancy QTL analysis germplasm enhancement |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1270925/full |
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