Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species
<i>Raffinose synthase</i> (<i>Rafs</i>) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the <...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/13/11120 |
| Summary: | <i>Raffinose synthase</i> (<i>Rafs</i>) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the <i>Rafs</i> families and profiled their alternative splicing patterns at the genome-wide scale from 10 grass species representing crops and grasses. A total of 73 <i>Rafs</i> genes were identified from grass species such as rice, maize, foxtail millet, and switchgrass. These <i>Rafs</i> genes were assigned to six groups based the phylogenetic analysis. We compared the gene structures, protein domains, and expression patterns of <i>Rafs</i> genes, and also unraveled the alternative transcripts of them. In addition, different conserved sequences were observed at these putative splice sites among grass species. The subcellular localization of <i>PvRafs5</i> suggested that the <i>Rafs</i> gene was expressed in the cytoplasm or cell membrane. Our findings provide comprehensive knowledge of the <i>Rafs</i> families in terms of genes and proteins, which will facilitate further functional characterization in grass species in response to abiotic stress. |
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| ISSN: | 1661-6596 1422-0067 |