Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species
<i>Raffinose synthase</i> (<i>Rafs</i>) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the <...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/11120 |
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| author | Junhao Xu Xiangkai You Yanan Leng Youyue Li Zeyu Lu Yinan Huang Moxian Chen Jianhua Zhang Tao Song Tieyuan Liu |
| author_facet | Junhao Xu Xiangkai You Yanan Leng Youyue Li Zeyu Lu Yinan Huang Moxian Chen Jianhua Zhang Tao Song Tieyuan Liu |
| author_sort | Junhao Xu |
| collection | DOAJ |
| description | <i>Raffinose synthase</i> (<i>Rafs</i>) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the <i>Rafs</i> families and profiled their alternative splicing patterns at the genome-wide scale from 10 grass species representing crops and grasses. A total of 73 <i>Rafs</i> genes were identified from grass species such as rice, maize, foxtail millet, and switchgrass. These <i>Rafs</i> genes were assigned to six groups based the phylogenetic analysis. We compared the gene structures, protein domains, and expression patterns of <i>Rafs</i> genes, and also unraveled the alternative transcripts of them. In addition, different conserved sequences were observed at these putative splice sites among grass species. The subcellular localization of <i>PvRafs5</i> suggested that the <i>Rafs</i> gene was expressed in the cytoplasm or cell membrane. Our findings provide comprehensive knowledge of the <i>Rafs</i> families in terms of genes and proteins, which will facilitate further functional characterization in grass species in response to abiotic stress. |
| first_indexed | 2024-03-11T01:37:58Z |
| format | Article |
| id | doaj.art-407c778164ec457eb146ec056b3e7816 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:37:58Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-407c778164ec457eb146ec056b3e78162023-11-18T16:48:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124131112010.3390/ijms241311120Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass SpeciesJunhao Xu0Xiangkai You1Yanan Leng2Youyue Li3Zeyu Lu4Yinan Huang5Moxian Chen6Jianhua Zhang7Tao Song8Tieyuan Liu9College of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210000, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210000, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong KongCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210000, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, China<i>Raffinose synthase</i> (<i>Rafs</i>) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the <i>Rafs</i> families and profiled their alternative splicing patterns at the genome-wide scale from 10 grass species representing crops and grasses. A total of 73 <i>Rafs</i> genes were identified from grass species such as rice, maize, foxtail millet, and switchgrass. These <i>Rafs</i> genes were assigned to six groups based the phylogenetic analysis. We compared the gene structures, protein domains, and expression patterns of <i>Rafs</i> genes, and also unraveled the alternative transcripts of them. In addition, different conserved sequences were observed at these putative splice sites among grass species. The subcellular localization of <i>PvRafs5</i> suggested that the <i>Rafs</i> gene was expressed in the cytoplasm or cell membrane. Our findings provide comprehensive knowledge of the <i>Rafs</i> families in terms of genes and proteins, which will facilitate further functional characterization in grass species in response to abiotic stress.https://www.mdpi.com/1422-0067/24/13/11120raffinose synthasegrass speciesgene familyalternative splicingdrought and salt stress |
| spellingShingle | Junhao Xu Xiangkai You Yanan Leng Youyue Li Zeyu Lu Yinan Huang Moxian Chen Jianhua Zhang Tao Song Tieyuan Liu Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species International Journal of Molecular Sciences raffinose synthase grass species gene family alternative splicing drought and salt stress |
| title | Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species |
| title_full | Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species |
| title_fullStr | Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species |
| title_full_unstemmed | Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species |
| title_short | Identification and Alternative Splicing Profile of the <i>Raffinose synthase</i> Gene in Grass Species |
| title_sort | identification and alternative splicing profile of the i raffinose synthase i gene in grass species |
| topic | raffinose synthase grass species gene family alternative splicing drought and salt stress |
| url | https://www.mdpi.com/1422-0067/24/13/11120 |
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