Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae
Aedes aegypti is a mosquito that carries dengue virus, yellow fever and other diseases transmitted to humans. Organophosphorus larvicides are used to control the proliferation of this mosquito, which has generated a high degree of resistance; hence, new alternatives such as bio-larvicides formulated...
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Colombia, Sede Medellín
2020-09-01
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| Series: | Revista Facultad Nacional de Agronomía Medellín |
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| Online Access: | https://revistas.unal.edu.co/index.php/refame/article/view/80501 |
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| author | Patricia Manzano Oscar Valmaña García Jessica Malusín Jenny Villamar María Quijano Rafael Viteri Ana Barragán Andrea Orellana-Manzano |
| author_facet | Patricia Manzano Oscar Valmaña García Jessica Malusín Jenny Villamar María Quijano Rafael Viteri Ana Barragán Andrea Orellana-Manzano |
| author_sort | Patricia Manzano |
| collection | DOAJ |
| description | Aedes aegypti is a mosquito that carries dengue virus, yellow fever and other diseases transmitted to humans. Organophosphorus larvicides are used to control the proliferation of this mosquito, which has generated a high degree of resistance; hence, new alternatives such as bio-larvicides formulated with plant extracts are of great interest. The aims of this study were to evaluate the ethanolic extract of Azadirachta indica leaves as a larvicide against Aedes aegypti and to determine the main compounds present in it by GC-MS. In the assay, three concentrations of ethanolic extract were used (10 mg L-1, 20 mg L-1, and 50 mg L-1). This was performed thrice against a positive control (commercial larvicide: spores and endotoxic crystals of Bacillus thuringiensis var. israelensis Serotype H-14) and negative control (water). After 72 h of incubation, it was observed higher larval mortality (93%) in the ethanolic extract at a concentration of 50 mg L-1; the extracts at 10 mg L-1 and 20 mg L-1 shown larval mortality of 47% and 70%, respectively. The majority compound determined by the GC-MS analysis was phytol (14.4% area). The results obtained in this study demonstrated the larvicidal potential of the ethanolic extract of A. indica against larvae of A. aegypti. |
| first_indexed | 2024-12-24T00:03:25Z |
| format | Article |
| id | doaj.art-407fe7fd11ca4c10b8885f3b1bc0e169 |
| institution | Directory Open Access Journal |
| issn | 0304-2847 2248-7026 |
| language | English |
| last_indexed | 2024-12-24T00:03:25Z |
| publishDate | 2020-09-01 |
| publisher | Universidad Nacional de Colombia, Sede Medellín |
| record_format | Article |
| series | Revista Facultad Nacional de Agronomía Medellín |
| spelling | doaj.art-407fe7fd11ca4c10b8885f3b1bc0e1692022-12-21T17:25:03ZengUniversidad Nacional de Colombia, Sede MedellínRevista Facultad Nacional de Agronomía Medellín0304-28472248-70262020-09-017339315932010.15446/rfnam.v73n3.8050153949Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvaePatricia Manzano0Oscar Valmaña García1Jessica Malusín2Jenny Villamar3María Quijano4Rafael Viteri5Ana Barragán6Andrea Orellana-Manzano7Escuela Superior Politecnica del LitoralUniversidad de GuayaquilUniversidad de GuayaquilUniversidad de GuayaquilEscuela Superior Politecnica del LitoralEscuela Superior Politecnica del LitoralEscuela Superior Politecnica del LitoralEscuela Superior Politecnica del LitoralAedes aegypti is a mosquito that carries dengue virus, yellow fever and other diseases transmitted to humans. Organophosphorus larvicides are used to control the proliferation of this mosquito, which has generated a high degree of resistance; hence, new alternatives such as bio-larvicides formulated with plant extracts are of great interest. The aims of this study were to evaluate the ethanolic extract of Azadirachta indica leaves as a larvicide against Aedes aegypti and to determine the main compounds present in it by GC-MS. In the assay, three concentrations of ethanolic extract were used (10 mg L-1, 20 mg L-1, and 50 mg L-1). This was performed thrice against a positive control (commercial larvicide: spores and endotoxic crystals of Bacillus thuringiensis var. israelensis Serotype H-14) and negative control (water). After 72 h of incubation, it was observed higher larval mortality (93%) in the ethanolic extract at a concentration of 50 mg L-1; the extracts at 10 mg L-1 and 20 mg L-1 shown larval mortality of 47% and 70%, respectively. The majority compound determined by the GC-MS analysis was phytol (14.4% area). The results obtained in this study demonstrated the larvicidal potential of the ethanolic extract of A. indica against larvae of A. aegypti.https://revistas.unal.edu.co/index.php/refame/article/view/80501bio-larvicidecrude ethanolic extractgc-msmosquitoneemphytol |
| spellingShingle | Patricia Manzano Oscar Valmaña García Jessica Malusín Jenny Villamar María Quijano Rafael Viteri Ana Barragán Andrea Orellana-Manzano Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae Revista Facultad Nacional de Agronomía Medellín bio-larvicide crude ethanolic extract gc-ms mosquito neem phytol |
| title | Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae |
| title_full | Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae |
| title_fullStr | Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae |
| title_full_unstemmed | Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae |
| title_short | Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae |
| title_sort | larvicidal activity of ethanolic extract of azadirachta indica against aedes aegypti larvae |
| topic | bio-larvicide crude ethanolic extract gc-ms mosquito neem phytol |
| url | https://revistas.unal.edu.co/index.php/refame/article/view/80501 |
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