Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance
RNA N6-melthyladenosine (m6A) can play an important role in regulation of various biological processes. Chicken ovary development is closely related to egg laying performance, which is a process primarily controlled by complex gene regulations. In this study, transcriptome-wide m6A methylation of th...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1284554/full |
| _version_ | 1797655163028111360 |
|---|---|
| author | Congjun Jia Congjun Jia Mengling Zhang Mengling Zhang Xiaoyan Liu Xiaoyan Liu Weilin Xu Weilin Xu Yanqing Xiong Yanqing Xiong Rihao Huang Rihao Huang Meidi Li Meidi Li Mingna Li |
| author_facet | Congjun Jia Congjun Jia Mengling Zhang Mengling Zhang Xiaoyan Liu Xiaoyan Liu Weilin Xu Weilin Xu Yanqing Xiong Yanqing Xiong Rihao Huang Rihao Huang Meidi Li Meidi Li Mingna Li |
| author_sort | Congjun Jia |
| collection | DOAJ |
| description | RNA N6-melthyladenosine (m6A) can play an important role in regulation of various biological processes. Chicken ovary development is closely related to egg laying performance, which is a process primarily controlled by complex gene regulations. In this study, transcriptome-wide m6A methylation of the Wuhua yellow-feathered chicken ovaries before and after sexual maturation was profiled to identify the potential molecular mechanisms underlying chicken ovary development. The results indicated that m6A levels of mRNAs were altered dramatically during sexual maturity. A total of 1,476 differential m6A peaks were found between these two stages with 662 significantly upregulated methylation peaks and 814 downregulated methylation peaks after sexual maturation. A positive correlation was observed between the m6A peaks and gene expression levels, indicating that m6A may play an important role in regulation of chicken ovary development. Functional enrichment analysis indicated that apoptosis related pathways could be the key molecular regulatory pathway underlying the poor reproductive performance of Wuhua yellow-feathered chicken. Overall, the various pathways and corresponding candidate genes identified here could be useful to facilitate molecular design breeding for improving egg production performance in Chinese local chicken breed, and it might also contribute to the genetic resource protection of valuable avian species. |
| first_indexed | 2024-03-11T17:10:10Z |
| format | Article |
| id | doaj.art-4082bee955ba4eb38904587ccdbc26a7 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-11T17:10:10Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-4082bee955ba4eb38904587ccdbc26a72023-10-20T06:03:33ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-10-011410.3389/fgene.2023.12845541284554Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performanceCongjun Jia0Congjun Jia1Mengling Zhang2Mengling Zhang3Xiaoyan Liu4Xiaoyan Liu5Weilin Xu6Weilin Xu7Yanqing Xiong8Yanqing Xiong9Rihao Huang10Rihao Huang11Meidi Li12Meidi Li13Mingna Li14College of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Agricultural Engineering, Guangdong Meizhou Vocational and Technical College, Meizhou, ChinaMeizhou Engineering Research Center for Veterinary Medicine and Natural Medicine, Meizhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaRNA N6-melthyladenosine (m6A) can play an important role in regulation of various biological processes. Chicken ovary development is closely related to egg laying performance, which is a process primarily controlled by complex gene regulations. In this study, transcriptome-wide m6A methylation of the Wuhua yellow-feathered chicken ovaries before and after sexual maturation was profiled to identify the potential molecular mechanisms underlying chicken ovary development. The results indicated that m6A levels of mRNAs were altered dramatically during sexual maturity. A total of 1,476 differential m6A peaks were found between these two stages with 662 significantly upregulated methylation peaks and 814 downregulated methylation peaks after sexual maturation. A positive correlation was observed between the m6A peaks and gene expression levels, indicating that m6A may play an important role in regulation of chicken ovary development. Functional enrichment analysis indicated that apoptosis related pathways could be the key molecular regulatory pathway underlying the poor reproductive performance of Wuhua yellow-feathered chicken. Overall, the various pathways and corresponding candidate genes identified here could be useful to facilitate molecular design breeding for improving egg production performance in Chinese local chicken breed, and it might also contribute to the genetic resource protection of valuable avian species.https://www.frontiersin.org/articles/10.3389/fgene.2023.1284554/fullWuhua yellow-feathered chickenovarym6A methylationsexual maturationreproduction |
| spellingShingle | Congjun Jia Congjun Jia Mengling Zhang Mengling Zhang Xiaoyan Liu Xiaoyan Liu Weilin Xu Weilin Xu Yanqing Xiong Yanqing Xiong Rihao Huang Rihao Huang Meidi Li Meidi Li Mingna Li Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance Frontiers in Genetics Wuhua yellow-feathered chicken ovary m6A methylation sexual maturation reproduction |
| title | Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance |
| title_full | Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance |
| title_fullStr | Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance |
| title_full_unstemmed | Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance |
| title_short | Transcriptome-wide m6A methylation profiling of Wuhua yellow-feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg-laying performance |
| title_sort | transcriptome wide m6a methylation profiling of wuhua yellow feathered chicken ovary revealed regulatory pathways underlying sexual maturation and low egg laying performance |
| topic | Wuhua yellow-feathered chicken ovary m6A methylation sexual maturation reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1284554/full |
| work_keys_str_mv | AT congjunjia transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT congjunjia transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT menglingzhang transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT menglingzhang transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT xiaoyanliu transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT xiaoyanliu transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT weilinxu transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT weilinxu transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT yanqingxiong transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT yanqingxiong transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT rihaohuang transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT rihaohuang transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT meidili transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT meidili transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance AT mingnali transcriptomewidem6amethylationprofilingofwuhuayellowfeatheredchickenovaryrevealedregulatorypathwaysunderlyingsexualmaturationandlowegglayingperformance |