Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation
We produced a tear proteome of the genome mouse, C57BL/6, that contained 139 different protein identifications: 110 from a two-dimensional (2D) gel with subsequent trypsin digestion, 19 from a one-dimensional (1D) gel with subsequent trypsin digestion and ten from a 1D gel with subsequent Asp-N dige...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-09-01
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| Series: | Proteomes |
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| Online Access: | http://www.mdpi.com/2227-7382/3/3/283 |
| _version_ | 1811187880902524928 |
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| author | Robert C. Karn Christina M. Laukaitis |
| author_facet | Robert C. Karn Christina M. Laukaitis |
| author_sort | Robert C. Karn |
| collection | DOAJ |
| description | We produced a tear proteome of the genome mouse, C57BL/6, that contained 139 different protein identifications: 110 from a two-dimensional (2D) gel with subsequent trypsin digestion, 19 from a one-dimensional (1D) gel with subsequent trypsin digestion and ten from a 1D gel with subsequent Asp-N digestion. We compared this tear proteome with a C57BL/6 mouse saliva proteome produced previously. Sixteen of the 139 tear proteins are shared between the two proteomes, including six proteins that combat microbial growth. Among the 123 other tear proteins, were members of four large protein families that have no counterparts in humans: Androgen-binding proteins (ABPs) with different members expressed in the two proteomes, Exocrine secreted peptides (ESPs) expressed exclusively in the tear proteome, major urinary proteins (MUPs) expressed in one or both proteomes and the mouse-specific Kallikreins (subfamily b KLKs) expressed exclusively in the saliva proteome. All four families have members with suggested roles in mouse communication, which may influence some aspect of reproductive behavior. We discuss this in the context of functional adaptation involving tear and saliva proteins in the secretions of mouse lacrimal and salivary glands, respectively. |
| first_indexed | 2024-04-11T14:10:05Z |
| format | Article |
| id | doaj.art-4087fd12b3004b1e9d471e8ff5ed42b8 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T14:10:05Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-4087fd12b3004b1e9d471e8ff5ed42b82022-12-22T04:19:43ZengMDPI AGProteomes2227-73822015-09-013328329710.3390/proteomes3030283proteomes3030283Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional AdaptationRobert C. Karn0Christina M. Laukaitis1College of Medicine, University of Arizona, Tucson, AZ 85724, USACollege of Medicine, University of Arizona, Tucson, AZ 85724, USAWe produced a tear proteome of the genome mouse, C57BL/6, that contained 139 different protein identifications: 110 from a two-dimensional (2D) gel with subsequent trypsin digestion, 19 from a one-dimensional (1D) gel with subsequent trypsin digestion and ten from a 1D gel with subsequent Asp-N digestion. We compared this tear proteome with a C57BL/6 mouse saliva proteome produced previously. Sixteen of the 139 tear proteins are shared between the two proteomes, including six proteins that combat microbial growth. Among the 123 other tear proteins, were members of four large protein families that have no counterparts in humans: Androgen-binding proteins (ABPs) with different members expressed in the two proteomes, Exocrine secreted peptides (ESPs) expressed exclusively in the tear proteome, major urinary proteins (MUPs) expressed in one or both proteomes and the mouse-specific Kallikreins (subfamily b KLKs) expressed exclusively in the saliva proteome. All four families have members with suggested roles in mouse communication, which may influence some aspect of reproductive behavior. We discuss this in the context of functional adaptation involving tear and saliva proteins in the secretions of mouse lacrimal and salivary glands, respectively.http://www.mdpi.com/2227-7382/3/3/283mousetearssalivaproteomelacrimal glandsalivary glandsadaptation |
| spellingShingle | Robert C. Karn Christina M. Laukaitis Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation Proteomes mouse tears saliva proteome lacrimal gland salivary glands adaptation |
| title | Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation |
| title_full | Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation |
| title_fullStr | Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation |
| title_full_unstemmed | Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation |
| title_short | Comparative Proteomics of Mouse Tears and Saliva: Evidence from Large Protein Families for Functional Adaptation |
| title_sort | comparative proteomics of mouse tears and saliva evidence from large protein families for functional adaptation |
| topic | mouse tears saliva proteome lacrimal gland salivary glands adaptation |
| url | http://www.mdpi.com/2227-7382/3/3/283 |
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