Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
Abstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls an...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-94747-8 |
| _version_ | 1819200337792204800 |
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| author | Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing |
| author_facet | Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing |
| author_sort | Signe Abitz Winther |
| collection | DOAJ |
| description | Abstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30–299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications. |
| first_indexed | 2024-12-23T03:30:38Z |
| format | Article |
| id | doaj.art-408992ee5356447c8bc81c5b73641639 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:30:38Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-408992ee5356447c8bc81c5b736416392022-12-21T18:01:42ZengNature PortfolioScientific Reports2045-23222021-07-0111111110.1038/s41598-021-94747-8Faecal biomarkers in type 1 diabetes with and without diabetic nephropathySigne Abitz Winther0Miia Maininki Mannerla1Marie Frimodt-Møller2Frederik Persson3Tine Willum Hansen4Markku Lehto5Sohvi Hörkkö6Michael Blaut7Carol Forsblom8Per-Henrik Groop9Peter Rossing10Steno Diabetes Center CopenhagenFolkhälsan Institute of Genetics, Folkhälsan Research CenterSteno Diabetes Center CopenhagenSteno Diabetes Center CopenhagenSteno Diabetes Center CopenhagenFolkhälsan Institute of Genetics, Folkhälsan Research CenterMedical Microbiology and Immunology, Unit of Biomedicine, University of OuluDepartment of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-RehbrueckeFolkhälsan Institute of Genetics, Folkhälsan Research CenterFolkhälsan Institute of Genetics, Folkhälsan Research CenterSteno Diabetes Center CopenhagenAbstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30–299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.https://doi.org/10.1038/s41598-021-94747-8 |
| spellingShingle | Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy Scientific Reports |
| title | Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| title_full | Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| title_fullStr | Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| title_full_unstemmed | Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| title_short | Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| title_sort | faecal biomarkers in type 1 diabetes with and without diabetic nephropathy |
| url | https://doi.org/10.1038/s41598-021-94747-8 |
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