Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene
To explore the differences in body-weight traits of five goose breeds and analyze their genetic diversity and historical dynamics, we collected body-weight data statistics and used Sanger sequencing to determine the mitochondrial DNA of 100 samples of five typical goose breeds in China and abroad. T...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2073-4425/14/8/1605 |
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| author | Yang Zhang Shangzong Qi Linyu Liu Qiang Bao Teng Wu Wei Liu Yu Zhang Wenming Zhao Qi Xu Guohong Chen |
| author_facet | Yang Zhang Shangzong Qi Linyu Liu Qiang Bao Teng Wu Wei Liu Yu Zhang Wenming Zhao Qi Xu Guohong Chen |
| author_sort | Yang Zhang |
| collection | DOAJ |
| description | To explore the differences in body-weight traits of five goose breeds and analyze their genetic diversity and historical dynamics, we collected body-weight data statistics and used Sanger sequencing to determine the mitochondrial DNA of 100 samples of five typical goose breeds in China and abroad. The results indicated that Lion-Head, Hortobagy, and Yangzhou geese have great breeding potential for body weight. Thirteen polymorphic sites were detected in the corrected 505 bp sequence of the mitochondrial DNA (mtDNA) ND6 gene, accounting for approximately 2.57% of the total number of sites. The guanine-cytosine (GC) content (51.7%) of the whole sequence was higher than the adenine-thymine (AT) content (48.3%), showing a certain GC base preference. There were 11 haplotypes among the five breeds, including one shared haplotype. We analyzed the differences in the distribution of base mismatches among the five breeds and conducted Tajima’s D and Fu’s Fs neutral tests on the historical dynamics of the populations. The distribution of the mismatch difference presented an unsmooth single peak and the Tajima’s D value of the neutral test was negative (D < 0) and reached a significant level, which proves that the population of the three species had expanded; the Lion-Head goose population tends to be stable. The genetic diversity of Lion-Head, Zhedong White, Yangzhou, and Taihu geese was equal to the average diversity of Chinese goose breeds. The Hortobagy goose is a foreign breed with differences in mating line breeding and hybrid advantage utilization. |
| first_indexed | 2024-03-10T23:54:10Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T23:54:10Z |
| publishDate | 2023-08-01 |
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| series | Genes |
| spelling | doaj.art-408c30dd18454f9aaabeeedae6a3c4cc2023-11-19T01:15:47ZengMDPI AGGenes2073-44252023-08-01148160510.3390/genes14081605Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 GeneYang Zhang0Shangzong Qi1Linyu Liu2Qiang Bao3Teng Wu4Wei Liu5Yu Zhang6Wenming Zhao7Qi Xu8Guohong Chen9Key Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Evaluation and Utilization of Poultry Genetic Resources of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, ChinaTo explore the differences in body-weight traits of five goose breeds and analyze their genetic diversity and historical dynamics, we collected body-weight data statistics and used Sanger sequencing to determine the mitochondrial DNA of 100 samples of five typical goose breeds in China and abroad. The results indicated that Lion-Head, Hortobagy, and Yangzhou geese have great breeding potential for body weight. Thirteen polymorphic sites were detected in the corrected 505 bp sequence of the mitochondrial DNA (mtDNA) ND6 gene, accounting for approximately 2.57% of the total number of sites. The guanine-cytosine (GC) content (51.7%) of the whole sequence was higher than the adenine-thymine (AT) content (48.3%), showing a certain GC base preference. There were 11 haplotypes among the five breeds, including one shared haplotype. We analyzed the differences in the distribution of base mismatches among the five breeds and conducted Tajima’s D and Fu’s Fs neutral tests on the historical dynamics of the populations. The distribution of the mismatch difference presented an unsmooth single peak and the Tajima’s D value of the neutral test was negative (D < 0) and reached a significant level, which proves that the population of the three species had expanded; the Lion-Head goose population tends to be stable. The genetic diversity of Lion-Head, Zhedong White, Yangzhou, and Taihu geese was equal to the average diversity of Chinese goose breeds. The Hortobagy goose is a foreign breed with differences in mating line breeding and hybrid advantage utilization.https://www.mdpi.com/2073-4425/14/8/1605local breedsmitochondrial DNAdifferences in body shapegenetic diversitydynamic analysis |
| spellingShingle | Yang Zhang Shangzong Qi Linyu Liu Qiang Bao Teng Wu Wei Liu Yu Zhang Wenming Zhao Qi Xu Guohong Chen Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene Genes local breeds mitochondrial DNA differences in body shape genetic diversity dynamic analysis |
| title | Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene |
| title_full | Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene |
| title_fullStr | Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene |
| title_full_unstemmed | Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene |
| title_short | Genetic Diversity Analysis and Breeding of Geese Based on the Mitochondrial ND6 Gene |
| title_sort | genetic diversity analysis and breeding of geese based on the mitochondrial nd6 gene |
| topic | local breeds mitochondrial DNA differences in body shape genetic diversity dynamic analysis |
| url | https://www.mdpi.com/2073-4425/14/8/1605 |
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