Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells
The serum level of soluble (s)E-cadherin is elevated in several malignancies, including prostate cancer (PCa). This study was designed to investigate the effects of sE-cadherin on the behavior of PCa cells in vitro, with the aim of identifying a potential therapeutic target. Growth as well as adhesi...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/10/1007 |
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| author | Igor Tsaur Anita Thomas Eva Juengel Sebastian Maxeiner Timothy Grein Quynh Chi Le Veronika Muschta Jochen Rutz Felix K. H. Chun Roman A. Blaheta |
| author_facet | Igor Tsaur Anita Thomas Eva Juengel Sebastian Maxeiner Timothy Grein Quynh Chi Le Veronika Muschta Jochen Rutz Felix K. H. Chun Roman A. Blaheta |
| author_sort | Igor Tsaur |
| collection | DOAJ |
| description | The serum level of soluble (s)E-cadherin is elevated in several malignancies, including prostate cancer (PCa). This study was designed to investigate the effects of sE-cadherin on the behavior of PCa cells in vitro, with the aim of identifying a potential therapeutic target. Growth as well as adhesive and motile behavior were evaluated in PC3, DU-145, and LNCaP cells. Flow cytometry was used to assess cell cycle phases and the surface expression of CD44 variants as well as α and β integrins. Confocal microscopy was utilized to visualize the distribution of CD44 variants within the cells. Western blot was applied to investigate expression of α3 and β1 integrins as well as cytoskeletal and adhesion proteins. Cell growth was significantly inhibited after exposure to 5 µg/mL sE-cadherin and was accompanied by a G0/G1-phase arrest. Adhesion of cells to collagen and fibronectin was mitigated, while motility was augmented. CD44v4, v5, and v7 expression was elevated while α3 and β1 integrins were attenuated. Blocking integrin α3 reduced cell growth and adhesion to collagen but increased motility. sE-cadherin therefore appears to foster invasive tumor cell behavior, and targeting it might serve as a novel and innovative concept to treat advanced PCa. |
| first_indexed | 2024-03-10T06:43:16Z |
| format | Article |
| id | doaj.art-4096ec2ba66a4e37835f6aa48dba26ac |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T06:43:16Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-4096ec2ba66a4e37835f6aa48dba26ac2023-11-22T17:28:20ZengMDPI AGBiology2079-77372021-10-011010100710.3390/biology10101007Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer CellsIgor Tsaur0Anita Thomas1Eva Juengel2Sebastian Maxeiner3Timothy Grein4Quynh Chi Le5Veronika Muschta6Jochen Rutz7Felix K. H. Chun8Roman A. Blaheta9Department of Urology and Pediatric Urology, University Medicine Mainz, 55131 Mainz, GermanyDepartment of Urology and Pediatric Urology, University Medicine Mainz, 55131 Mainz, GermanyDepartment of Urology and Pediatric Urology, University Medicine Mainz, 55131 Mainz, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyDepartment of Urology, University Hospital Frankfurt, Goethe University, 60590 Frankfurt, GermanyThe serum level of soluble (s)E-cadherin is elevated in several malignancies, including prostate cancer (PCa). This study was designed to investigate the effects of sE-cadherin on the behavior of PCa cells in vitro, with the aim of identifying a potential therapeutic target. Growth as well as adhesive and motile behavior were evaluated in PC3, DU-145, and LNCaP cells. Flow cytometry was used to assess cell cycle phases and the surface expression of CD44 variants as well as α and β integrins. Confocal microscopy was utilized to visualize the distribution of CD44 variants within the cells. Western blot was applied to investigate expression of α3 and β1 integrins as well as cytoskeletal and adhesion proteins. Cell growth was significantly inhibited after exposure to 5 µg/mL sE-cadherin and was accompanied by a G0/G1-phase arrest. Adhesion of cells to collagen and fibronectin was mitigated, while motility was augmented. CD44v4, v5, and v7 expression was elevated while α3 and β1 integrins were attenuated. Blocking integrin α3 reduced cell growth and adhesion to collagen but increased motility. sE-cadherin therefore appears to foster invasive tumor cell behavior, and targeting it might serve as a novel and innovative concept to treat advanced PCa.https://www.mdpi.com/2079-7737/10/10/1007prostate cancersE-cadherincell growthmigrationmetastasis |
| spellingShingle | Igor Tsaur Anita Thomas Eva Juengel Sebastian Maxeiner Timothy Grein Quynh Chi Le Veronika Muschta Jochen Rutz Felix K. H. Chun Roman A. Blaheta Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells Biology prostate cancer sE-cadherin cell growth migration metastasis |
| title | Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells |
| title_full | Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells |
| title_fullStr | Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells |
| title_full_unstemmed | Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells |
| title_short | Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells |
| title_sort | deciphering the molecular machinery influence of se cadherin on tumorigenic traits of prostate cancer cells |
| topic | prostate cancer sE-cadherin cell growth migration metastasis |
| url | https://www.mdpi.com/2079-7737/10/10/1007 |
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