Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius)
Small heat shock proteins (sHsps) are molecular chaperones that play crucial roles in the stress adaption of insects. In this study, we identified and characterized four <i>sHsp</i> genes (<i>LsHsp19.4</i>, <i>20.2</i>, <i>20.3</i>, and <i>22.2&l...
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MDPI AG
2019-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/10/5/139 |
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| author | Wen-Jia Yang Kang-Kang Xu Yu Cao Yong-Lu Meng Yan Liu Can Li |
| author_facet | Wen-Jia Yang Kang-Kang Xu Yu Cao Yong-Lu Meng Yan Liu Can Li |
| author_sort | Wen-Jia Yang |
| collection | DOAJ |
| description | Small heat shock proteins (sHsps) are molecular chaperones that play crucial roles in the stress adaption of insects. In this study, we identified and characterized four <i>sHsp</i> genes (<i>LsHsp19.4</i>, <i>20.2</i>, <i>20.3</i>, and <i>22.2</i>) from the cigarette beetle, <i>Lasioderma serricorne</i> (Fabricius). The four cDNAs encoded proteins of 169, 180, 181, and 194 amino acids with molecular weights of 19.4, 20.2, 20.3, and 22.2 kDa, respectively. The four <i>LsHsp</i> sequences possessed a typical sHsp domain structure. Quantitative real-time PCR analyses revealed that <i>LsHsp19.4</i> and <i>20.3</i> transcripts were most abundant in pupae, whereas the transcript levels of <i>LsHsp20.2</i> and <i>22.2</i> were highest in adults. Transcripts of three <i>LsHsp</i> genes were highly expressed in the larval fat body, whereas <i>LsHsp20.2</i> displayed an extremely high expression level in the gut. Expression of the four <i>LsHsp</i> genes was dramatically upregulated in larvae exposed to 20-hydroxyecdysone. The majority of the <i>LsHsp</i> genes were significantly upregulated in response to heat and cold treatments, while <i>LsHsp19.4</i> was insensitive to cold stress. The four genes were upregulated when challenged by immune triggers (peptidoglycan isolated from <i>Staphylococcus aureus</i> and from <i>Escherichia coli</i> 0111:B4). Exposure to CO<sub>2</sub> increased <i>LsHsp20.2</i> and <i>20.3</i> transcript levels, but the <i>LsHsp19.4</i> transcript level declined. The results suggest that different <i>LsHsp</i> genes play important and distinct regulatory roles in <i>L. serricorne</i> development and in response to diverse stresses. |
| first_indexed | 2024-12-21T12:46:50Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-12-21T12:46:50Z |
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| spelling | doaj.art-40979bd09a0c4b538b2a1ece8f9e32432022-12-21T19:03:36ZengMDPI AGInsects2075-44502019-05-0110513910.3390/insects10050139insects10050139Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius)Wen-Jia Yang0Kang-Kang Xu1Yu Cao2Yong-Lu Meng3Yan Liu4Can Li5Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, ChinaSmall heat shock proteins (sHsps) are molecular chaperones that play crucial roles in the stress adaption of insects. In this study, we identified and characterized four <i>sHsp</i> genes (<i>LsHsp19.4</i>, <i>20.2</i>, <i>20.3</i>, and <i>22.2</i>) from the cigarette beetle, <i>Lasioderma serricorne</i> (Fabricius). The four cDNAs encoded proteins of 169, 180, 181, and 194 amino acids with molecular weights of 19.4, 20.2, 20.3, and 22.2 kDa, respectively. The four <i>LsHsp</i> sequences possessed a typical sHsp domain structure. Quantitative real-time PCR analyses revealed that <i>LsHsp19.4</i> and <i>20.3</i> transcripts were most abundant in pupae, whereas the transcript levels of <i>LsHsp20.2</i> and <i>22.2</i> were highest in adults. Transcripts of three <i>LsHsp</i> genes were highly expressed in the larval fat body, whereas <i>LsHsp20.2</i> displayed an extremely high expression level in the gut. Expression of the four <i>LsHsp</i> genes was dramatically upregulated in larvae exposed to 20-hydroxyecdysone. The majority of the <i>LsHsp</i> genes were significantly upregulated in response to heat and cold treatments, while <i>LsHsp19.4</i> was insensitive to cold stress. The four genes were upregulated when challenged by immune triggers (peptidoglycan isolated from <i>Staphylococcus aureus</i> and from <i>Escherichia coli</i> 0111:B4). Exposure to CO<sub>2</sub> increased <i>LsHsp20.2</i> and <i>20.3</i> transcript levels, but the <i>LsHsp19.4</i> transcript level declined. The results suggest that different <i>LsHsp</i> genes play important and distinct regulatory roles in <i>L. serricorne</i> development and in response to diverse stresses.https://www.mdpi.com/2075-4450/10/5/139<i>Lasioderma serricorne</i>small heat shock proteindevelopmentstress responsesexpression pattern |
| spellingShingle | Wen-Jia Yang Kang-Kang Xu Yu Cao Yong-Lu Meng Yan Liu Can Li Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) Insects <i>Lasioderma serricorne</i> small heat shock protein development stress responses expression pattern |
| title | Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) |
| title_full | Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) |
| title_fullStr | Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) |
| title_full_unstemmed | Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) |
| title_short | Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, <i>Lasioderma serricorne</i> (Fabricius) |
| title_sort | identification and expression analysis of four small heat shock protein genes in cigarette beetle i lasioderma serricorne i fabricius |
| topic | <i>Lasioderma serricorne</i> small heat shock protein development stress responses expression pattern |
| url | https://www.mdpi.com/2075-4450/10/5/139 |
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