Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division
Bouquet formation is believed to be involved in initiating homologous chromosome pairings in meiosis. A bouquet is also formed in the absence of chromosome pairing, such as in F<sub>1</sub> wheat–rye hybrids. In some hybrids, meiosis is characterized by a single, mitotic-like division th...
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MDPI AG
2022-06-01
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| author | Olga G. Silkova Dina B. Loginova Anastasia A. Zhuravleva Vladimir K. Shumny |
| author_facet | Olga G. Silkova Dina B. Loginova Anastasia A. Zhuravleva Vladimir K. Shumny |
| author_sort | Olga G. Silkova |
| collection | DOAJ |
| description | Bouquet formation is believed to be involved in initiating homologous chromosome pairings in meiosis. A bouquet is also formed in the absence of chromosome pairing, such as in F<sub>1</sub> wheat–rye hybrids. In some hybrids, meiosis is characterized by a single, mitotic-like division that leads to the formation of unreduced gametes. In this study, FISH with the telomere and centromere-specific probe, and immunoFISH with ASY1, CENH3 and rye subtelomere repeat pSc200 were employed to perform a comparative analysis of early meiotic prophase nuclei in four combinations of wheat–rye hybrids. One of these, with disomic rye chromosome 2R, is known to undergo normal meiosis, and here, 78.9% of the meiocytes formed a normal-appearing telomere bouquet and rye subtelomeres clustered in 83.2% of the meiocytes. In three combinations with disomic rye chromosomes 1R, 5R and 6R, known to undergo a single division of meiosis, telomeres clustered in 11.4%, 44.8% and 27.6% of the meiocytes, respectively. In hybrids with chromosome 1R, rye subtelomeres clustered in 12.19% of the meiocytes. In the remaining meiocytes, telomeres and subtelomeres were scattered along the nucleus circumference, forming large and small groups. We conclude that in wheat–rye hybrids with mitotic-like meiosis, chromosome behavior is altered already in the early prophase. |
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| language | English |
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| spelling | doaj.art-4098fe16b60c493ca0b35be3f1182d832023-11-23T18:34:53ZengMDPI AGPlants2223-77472022-06-011112158210.3390/plants11121582Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like DivisionOlga G. Silkova0Dina B. Loginova1Anastasia A. Zhuravleva2Vladimir K. Shumny3Department of Plant Genetics, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaDepartment of Plant Genetics, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaDepartment of Plant Genetics, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaDepartment of Plant Genetics, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaBouquet formation is believed to be involved in initiating homologous chromosome pairings in meiosis. A bouquet is also formed in the absence of chromosome pairing, such as in F<sub>1</sub> wheat–rye hybrids. In some hybrids, meiosis is characterized by a single, mitotic-like division that leads to the formation of unreduced gametes. In this study, FISH with the telomere and centromere-specific probe, and immunoFISH with ASY1, CENH3 and rye subtelomere repeat pSc200 were employed to perform a comparative analysis of early meiotic prophase nuclei in four combinations of wheat–rye hybrids. One of these, with disomic rye chromosome 2R, is known to undergo normal meiosis, and here, 78.9% of the meiocytes formed a normal-appearing telomere bouquet and rye subtelomeres clustered in 83.2% of the meiocytes. In three combinations with disomic rye chromosomes 1R, 5R and 6R, known to undergo a single division of meiosis, telomeres clustered in 11.4%, 44.8% and 27.6% of the meiocytes, respectively. In hybrids with chromosome 1R, rye subtelomeres clustered in 12.19% of the meiocytes. In the remaining meiocytes, telomeres and subtelomeres were scattered along the nucleus circumference, forming large and small groups. We conclude that in wheat–rye hybrids with mitotic-like meiosis, chromosome behavior is altered already in the early prophase.https://www.mdpi.com/2223-7747/11/12/1582wheat–rye amphihaploidsmitotic-like meiosistelomerescentromeressubtelomeresbouquet |
| spellingShingle | Olga G. Silkova Dina B. Loginova Anastasia A. Zhuravleva Vladimir K. Shumny Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division Plants wheat–rye amphihaploids mitotic-like meiosis telomeres centromeres subtelomeres bouquet |
| title | Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division |
| title_full | Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division |
| title_fullStr | Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division |
| title_full_unstemmed | Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division |
| title_short | Bouquet Formation Failure in Meiosis of F<sub>1</sub> Wheat–Rye Hybrids with Mitotic-Like Division |
| title_sort | bouquet formation failure in meiosis of f sub 1 sub wheat rye hybrids with mitotic like division |
| topic | wheat–rye amphihaploids mitotic-like meiosis telomeres centromeres subtelomeres bouquet |
| url | https://www.mdpi.com/2223-7747/11/12/1582 |
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