Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles
<p>Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicl...
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| Format: | Article |
| Language: | English |
| Published: |
Ivyspring International Publisher
2013-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v09p0142.htm |
| _version_ | 1828336161162002432 |
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| author | Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi |
| author_facet | Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi |
| author_sort | Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi |
| collection | DOAJ |
| description | <p>Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC<sub>50</sub>); <i>i.e.</i> IC<sub>50</sub> values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.</p> |
| first_indexed | 2024-04-13T21:57:08Z |
| format | Article |
| id | doaj.art-409a0bcf36944b29af4d31bd60e6a2b0 |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-04-13T21:57:08Z |
| publishDate | 2013-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-409a0bcf36944b29af4d31bd60e6a2b02022-12-22T02:28:13ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882013-01-0192142148Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) VesiclesKeita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi<p>Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC<sub>50</sub>); <i>i.e.</i> IC<sub>50</sub> values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.</p>http://www.biolsci.org/v09p0142.htm |
| spellingShingle | Keita Hayashi, Tsuyoshi Tatsui, Toshinori Shimanouchi, Hiroshi Umakoshi Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles International Journal of Biological Sciences |
| title | Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles |
| title_full | Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles |
| title_fullStr | Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles |
| title_full_unstemmed | Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles |
| title_short | Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles |
| title_sort | enhanced cytotoxicity for colon 26 cells using doxorubicin loaded sorbitan monooleate span 80 vesicles |
| url | http://www.biolsci.org/v09p0142.htm |
| work_keys_str_mv | AT keitahayashitsuyoshitatsuitoshinorishimanouchihiroshiumakoshi enhancedcytotoxicityforcolon26cellsusingdoxorubicinloadedsorbitanmonooleatespan80vesicles |