Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results
The majority of human diseases attributed to seafood are caused by Vibrio spp. and the most commonly reported species are Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae. The conventional methods for the detection of Vibrio species involve the use of selective media, which are inexp...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2024-02-01
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| Series: | Italian Journal of Food Safety |
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| Online Access: | https://www.pagepressjournals.org/index.php/ijfs/article/view/11635 |
| _version_ | 1797294391383031808 |
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| author | Orlandina Di Maro Yolande T.R. Proroga Silvia Castellano Anna Balestrieri Federico Capuano Enrico Arletti Michelangelo Vietina Melissa Bizzarri Nicoletta Murru Maria Francesca Peruzy Daniela Cristiano |
| author_facet | Orlandina Di Maro Yolande T.R. Proroga Silvia Castellano Anna Balestrieri Federico Capuano Enrico Arletti Michelangelo Vietina Melissa Bizzarri Nicoletta Murru Maria Francesca Peruzy Daniela Cristiano |
| author_sort | Orlandina Di Maro |
| collection | DOAJ |
| description |
The majority of human diseases attributed to seafood are caused by Vibrio spp. and the most commonly reported species are Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae. The conventional methods for the detection of Vibrio species involve the use of selective media, which are inexpensive and simple but time-consuming. The present work aimed to develop a rapid method based on the use of multiplex real-time polymerase chain reaction (PCR) to detect V. parahaemolyticus, V. vulnificus, and V. cholerae in bivalve mollusks. 30 aliquots of bivalve mollusks (Mytilus galloprovincialis) were experimentally inoculated with two levels of V. parahaemolyticus, V. vulnificus, and V. cholerae. ISO 21872-1:2017 was used in parallel for qualitative analysis. The limit of detection of 50% was 7.67 CFU/g for V. cholerae, 0.024 CFU/g for V. vulnificus, and 1.36 CFU/g for V. parahaemolyticus. For V. vulnificus and V. cholerae, the real-time PCR protocol was demonstrated to amplify the pathogens in samples seeded with the lowest and highest levels. The molecular method evaluated showed a concordance rate of 100% with the reference microbiological method. V. parahaemolyticus was never detected in samples contaminated with the lowest level, and it was detected in 14 samples (93.33%) seeded with the highest concentration. In conclusion, the multiplex real-time PCR developed proved to be reliable for V. vulnificus and V. cholerae. Results for V. parahaemolyticus are promising, but further analysis is needed. The proposed method could represent a quick monitoring tool and, if used, would allow the implementation of food safety.
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| first_indexed | 2024-03-07T21:29:35Z |
| format | Article |
| id | doaj.art-409ac1d68c1f43df86ecb69652a9a023 |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-03-07T21:29:35Z |
| publishDate | 2024-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-409ac1d68c1f43df86ecb69652a9a0232024-02-26T23:11:40ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322024-02-0110.4081/ijfs.2024.11635Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary resultsOrlandina Di Maro0Yolande T.R. Proroga1Silvia Castellano2Anna Balestrieri3Federico Capuano4Enrico Arletti5Michelangelo Vietina6Melissa Bizzarri7Nicoletta Murru8Maria Francesca Peruzy9Daniela Cristiano10Department of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, PorticiDepartment of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, PorticiDepartment of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, PorticiDepartment of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, PorticiDepartment of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, PorticiGeneron S.p.A., San ProsperoGeneron S.p.A., San ProsperoGeneron S.p.A., San ProsperoDepartment of Veterinary Medicine and Animal Production, University of Naples Federico IIDepartment of Veterinary Medicine and Animal Production, University of Naples Federico IIDepartment of Food Inspection, Experimental Zooprophylactic Institute of Southern Italy, Portici The majority of human diseases attributed to seafood are caused by Vibrio spp. and the most commonly reported species are Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae. The conventional methods for the detection of Vibrio species involve the use of selective media, which are inexpensive and simple but time-consuming. The present work aimed to develop a rapid method based on the use of multiplex real-time polymerase chain reaction (PCR) to detect V. parahaemolyticus, V. vulnificus, and V. cholerae in bivalve mollusks. 30 aliquots of bivalve mollusks (Mytilus galloprovincialis) were experimentally inoculated with two levels of V. parahaemolyticus, V. vulnificus, and V. cholerae. ISO 21872-1:2017 was used in parallel for qualitative analysis. The limit of detection of 50% was 7.67 CFU/g for V. cholerae, 0.024 CFU/g for V. vulnificus, and 1.36 CFU/g for V. parahaemolyticus. For V. vulnificus and V. cholerae, the real-time PCR protocol was demonstrated to amplify the pathogens in samples seeded with the lowest and highest levels. The molecular method evaluated showed a concordance rate of 100% with the reference microbiological method. V. parahaemolyticus was never detected in samples contaminated with the lowest level, and it was detected in 14 samples (93.33%) seeded with the highest concentration. In conclusion, the multiplex real-time PCR developed proved to be reliable for V. vulnificus and V. cholerae. Results for V. parahaemolyticus are promising, but further analysis is needed. The proposed method could represent a quick monitoring tool and, if used, would allow the implementation of food safety. https://www.pagepressjournals.org/index.php/ijfs/article/view/11635V. parahaemolyticusV. vulnificusV. choleraereal-time PCR |
| spellingShingle | Orlandina Di Maro Yolande T.R. Proroga Silvia Castellano Anna Balestrieri Federico Capuano Enrico Arletti Michelangelo Vietina Melissa Bizzarri Nicoletta Murru Maria Francesca Peruzy Daniela Cristiano Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results Italian Journal of Food Safety V. parahaemolyticus V. vulnificus V. cholerae real-time PCR |
| title | Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results |
| title_full | Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results |
| title_fullStr | Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results |
| title_full_unstemmed | Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results |
| title_short | Detection of pathogenic <i>Vibrio</i> spp. in foods: polymerase chain reaction-based screening strategy to rapidly detect pathogenic <i>Vibrio parahaemolyticus</i>, <i>Vibrio cholerae</i>, and <i>Vibrio vulnificus</i> in bivalve mollusks and preliminary results |
| title_sort | detection of pathogenic i vibrio i spp in foods polymerase chain reaction based screening strategy to rapidly detect pathogenic i vibrio parahaemolyticus i i vibrio cholerae i and i vibrio vulnificus i in bivalve mollusks and preliminary results |
| topic | V. parahaemolyticus V. vulnificus V. cholerae real-time PCR |
| url | https://www.pagepressjournals.org/index.php/ijfs/article/view/11635 |
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