Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms
Abstract Background The plant Kingiodendron pinnatum (DC.) Harms, belonging to the family Fabaceae is endemic to the Western Ghats of India and is commonly used for various ailments, especially by the tribes. K. pinnatum is occasionally used as a substitute for Saraca asoca in Asokarishta, a well-kn...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-02-01
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| Series: | Clinical Phytoscience |
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| Online Access: | https://doi.org/10.1186/s40816-023-00356-5 |
| _version_ | 1797863190035431424 |
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| author | Chennattu M. Pareeth Nair Meera Prabha Silpa Kannoor M. Thara Achuthan C. Raghavamenon Thekkekara D. Babu |
| author_facet | Chennattu M. Pareeth Nair Meera Prabha Silpa Kannoor M. Thara Achuthan C. Raghavamenon Thekkekara D. Babu |
| author_sort | Chennattu M. Pareeth |
| collection | DOAJ |
| description | Abstract Background The plant Kingiodendron pinnatum (DC.) Harms, belonging to the family Fabaceae is endemic to the Western Ghats of India and is commonly used for various ailments, especially by the tribes. K. pinnatum is occasionally used as a substitute for Saraca asoca in Asokarishta, a well-known uterine tonic in Ayurveda. Recent studies revealed a pharmacological similarity between the plants. S. asoca is reported to have anti-cancer properties, but there are no reports on K. pinnatum except for antioxidant and antimicrobial activities. Therefore, the study is aimed to investigate the anticancer potential of the plant. Methods Cytotoxicity of methanolic bark extract of the plant was analysed on different cancer cell lines by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Dalton's lymphoma ascites (DLA) cell-induced solid and Ehrlich ascites carcinoma (EAC) cell-induced ascites tumour models in mice were used to study the antitumor potential. Phytochemical screening of the extract was also performed. Results The extract was found cytotoxic to DLA, EAC, HCT15, MDA-MB-231, T47D and PC3 with inhibitory concentration (IC50) values of 50.09, 74.74, 67.02, 119.22, 149.04 and 194.5 μg/mL, respectively. In the solid tumour model, a significant (P < 0.001) reduction in tumour weight of 0.7 ± 0.15 g was observed in 500 mg/kg b.wt. extract treated group compared to the control group (3.6 ± 0.24 g) by oral administration for 30 days. In the ascites tumour model, a high survival rate of 28.2 ± 8.72 days (P < 0.01) was found by the extract treatment compared to the control animals. Phytochemicals like alkaloids, flavonoids, phenols, phytosterols, saponins, tannins, steroids and terpenoids were detected in the extract. Conclusion Results obtained by the cytotoxic and anti-tumour studies revealed the anticancer potential of K. pinnatum. The plant exhibits more cytotoxicity towards cancer cell lines of the reproductive system such as the breast and prostate. |
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| format | Article |
| id | doaj.art-409e22bc30a34c7099a7b8a87de8597f |
| institution | Directory Open Access Journal |
| issn | 2199-1197 |
| language | English |
| last_indexed | 2024-04-09T22:31:34Z |
| publishDate | 2023-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Clinical Phytoscience |
| spelling | doaj.art-409e22bc30a34c7099a7b8a87de8597f2023-03-22T12:42:02ZengSpringerOpenClinical Phytoscience2199-11972023-02-01911910.1186/s40816-023-00356-5Analysis of anticancer potential of Kingiodendron pinnatum (DC.) HarmsChennattu M. Pareeth0Nair Meera1Prabha Silpa2Kannoor M. Thara3Achuthan C. Raghavamenon4Thekkekara D. Babu5Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre, University of Calicut)Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre, University of Calicut)Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre, University of Calicut)Department of Biotechnology, University of CalicutDepartment of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre, University of Calicut)Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre, University of Calicut)Abstract Background The plant Kingiodendron pinnatum (DC.) Harms, belonging to the family Fabaceae is endemic to the Western Ghats of India and is commonly used for various ailments, especially by the tribes. K. pinnatum is occasionally used as a substitute for Saraca asoca in Asokarishta, a well-known uterine tonic in Ayurveda. Recent studies revealed a pharmacological similarity between the plants. S. asoca is reported to have anti-cancer properties, but there are no reports on K. pinnatum except for antioxidant and antimicrobial activities. Therefore, the study is aimed to investigate the anticancer potential of the plant. Methods Cytotoxicity of methanolic bark extract of the plant was analysed on different cancer cell lines by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Dalton's lymphoma ascites (DLA) cell-induced solid and Ehrlich ascites carcinoma (EAC) cell-induced ascites tumour models in mice were used to study the antitumor potential. Phytochemical screening of the extract was also performed. Results The extract was found cytotoxic to DLA, EAC, HCT15, MDA-MB-231, T47D and PC3 with inhibitory concentration (IC50) values of 50.09, 74.74, 67.02, 119.22, 149.04 and 194.5 μg/mL, respectively. In the solid tumour model, a significant (P < 0.001) reduction in tumour weight of 0.7 ± 0.15 g was observed in 500 mg/kg b.wt. extract treated group compared to the control group (3.6 ± 0.24 g) by oral administration for 30 days. In the ascites tumour model, a high survival rate of 28.2 ± 8.72 days (P < 0.01) was found by the extract treatment compared to the control animals. Phytochemicals like alkaloids, flavonoids, phenols, phytosterols, saponins, tannins, steroids and terpenoids were detected in the extract. Conclusion Results obtained by the cytotoxic and anti-tumour studies revealed the anticancer potential of K. pinnatum. The plant exhibits more cytotoxicity towards cancer cell lines of the reproductive system such as the breast and prostate.https://doi.org/10.1186/s40816-023-00356-5Kingiodendron pinnatumSaraca asocaAsokaCytotoxicityAnti-tumourReproductive cancer |
| spellingShingle | Chennattu M. Pareeth Nair Meera Prabha Silpa Kannoor M. Thara Achuthan C. Raghavamenon Thekkekara D. Babu Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms Clinical Phytoscience Kingiodendron pinnatum Saraca asoca Asoka Cytotoxicity Anti-tumour Reproductive cancer |
| title | Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms |
| title_full | Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms |
| title_fullStr | Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms |
| title_full_unstemmed | Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms |
| title_short | Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms |
| title_sort | analysis of anticancer potential of kingiodendron pinnatum dc harms |
| topic | Kingiodendron pinnatum Saraca asoca Asoka Cytotoxicity Anti-tumour Reproductive cancer |
| url | https://doi.org/10.1186/s40816-023-00356-5 |
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