Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction
Buthus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer,...
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| Format: | Article |
| Language: | English |
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SciELO
2003-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004 |
| _version_ | 1828883397561286656 |
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| author | S. P. Gawade |
| author_facet | S. P. Gawade |
| author_sort | S. P. Gawade |
| collection | DOAJ |
| description | Buthus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer, pH 6.0. Toxin purity was determined on SDS slab gel electrophoresis. Effective concentration of C56 toxin was based on contraction paralysis units (CPU) in Drosophila 3rd instar larvae by microinjection (0.1 CPU/ml = 2 x 10-6 g/ml). The toxin-induced excitatory junctional potentials were studied for calcium dependency (0.2 mM to 1.2 mM Ca2+) in Drosophila Ringer. Excitatory junctional potential amplitude was increased with increasing calcium concentration; maximum increase in the frequency at 0.4 mM Ca2+/4 mM Mg2+ Drosophila Ringer. It was suggested that while amplitude of excitatory junctional potentials was increased with concentration, maximum frequency increase at 0.4 mMCa2+/4 mM Mg2+ Drosophila Ringer may be due to augmented Ca2+ influx in 0.4 mM Ca2+, when NMDA receptors were maximally activated in C56 toxin-treated Drosophila larval neuromuscular junction. |
| first_indexed | 2024-12-13T10:48:26Z |
| format | Article |
| id | doaj.art-409e3ab4f64147859db21e65df11abfe |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-13T10:48:26Z |
| publishDate | 2003-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-409e3ab4f64147859db21e65df11abfe2022-12-21T23:50:00ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992003-01-0191657510.1590/S1678-91992003000100004Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junctionS. P. GawadeButhus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer, pH 6.0. Toxin purity was determined on SDS slab gel electrophoresis. Effective concentration of C56 toxin was based on contraction paralysis units (CPU) in Drosophila 3rd instar larvae by microinjection (0.1 CPU/ml = 2 x 10-6 g/ml). The toxin-induced excitatory junctional potentials were studied for calcium dependency (0.2 mM to 1.2 mM Ca2+) in Drosophila Ringer. Excitatory junctional potential amplitude was increased with increasing calcium concentration; maximum increase in the frequency at 0.4 mM Ca2+/4 mM Mg2+ Drosophila Ringer. It was suggested that while amplitude of excitatory junctional potentials was increased with concentration, maximum frequency increase at 0.4 mMCa2+/4 mM Mg2+ Drosophila Ringer may be due to augmented Ca2+ influx in 0.4 mM Ca2+, when NMDA receptors were maximally activated in C56 toxin-treated Drosophila larval neuromuscular junction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004Mesobuthus tamulusC56 toxinDrosophila melanogasterneuromuscularminiature excitatory junctional potentials (MEJPs) |
| spellingShingle | S. P. Gawade Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction Journal of Venomous Animals and Toxins including Tropical Diseases Mesobuthus tamulus C56 toxin Drosophila melanogaster neuromuscular miniature excitatory junctional potentials (MEJPs) |
| title | Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction |
| title_full | Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction |
| title_fullStr | Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction |
| title_full_unstemmed | Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction |
| title_short | Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction |
| title_sort | excitatory effects of buthus c56 toxin on drosophila larval neuromuscular junction |
| topic | Mesobuthus tamulus C56 toxin Drosophila melanogaster neuromuscular miniature excitatory junctional potentials (MEJPs) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004 |
| work_keys_str_mv | AT spgawade excitatoryeffectsofbuthusc56toxinondrosophilalarvalneuromuscularjunction |