A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity
Abstract Virus spillover remains a major challenge to public health. A panel of SARS-CoV-2-related coronaviruses have been identified in pangolins, while the infectivity and pathogenicity of these pangolin-origin coronaviruses (pCoV) in humans remain largely unknown. Herein, we comprehensively chara...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2023-06-01
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| Series: | Cell Discovery |
| Online Access: | https://doi.org/10.1038/s41421-023-00557-9 |
| _version_ | 1797801565132685312 |
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| author | Xing-Yao Huang Qi Chen Meng-Xu Sun Hang-Yu Zhou Qing Ye Wu Chen Jin-Yu Peng Yi-Ni Qi Jun-Qiong Zhai Ying Tian Zi-Xin Liu Yi-Jiao Huang Yong-Qiang Deng Xiao-Feng Li Aiping Wu Xiao Yang Guan Yang Yongyi Shen Cheng-Feng Qin |
| author_facet | Xing-Yao Huang Qi Chen Meng-Xu Sun Hang-Yu Zhou Qing Ye Wu Chen Jin-Yu Peng Yi-Ni Qi Jun-Qiong Zhai Ying Tian Zi-Xin Liu Yi-Jiao Huang Yong-Qiang Deng Xiao-Feng Li Aiping Wu Xiao Yang Guan Yang Yongyi Shen Cheng-Feng Qin |
| author_sort | Xing-Yao Huang |
| collection | DOAJ |
| description | Abstract Virus spillover remains a major challenge to public health. A panel of SARS-CoV-2-related coronaviruses have been identified in pangolins, while the infectivity and pathogenicity of these pangolin-origin coronaviruses (pCoV) in humans remain largely unknown. Herein, we comprehensively characterized the infectivity and pathogenicity of a recent pCoV isolate (pCoV-GD01) in human cells and human tracheal epithelium organoids and established animal models in comparison with SARS-CoV-2. pCoV-GD01 showed similar infectivity to SARS-CoV-2 in human cells and organoids. Remarkably, intranasal inoculation of pCoV-GD01 caused severe lung pathological damage in hACE2 mice and could transmit among cocaged hamsters. Interestingly, in vitro neutralization assays and animal heterologous challenge experiments demonstrated that preexisting immunity induced by SARS-CoV-2 infection or vaccination was sufficient to provide at least partial cross-protection against pCoV-GD01 challenge. Our results provide direct evidence supporting pCoV-GD01 as a potential human pathogen and highlight the potential spillover risk. |
| first_indexed | 2024-03-13T04:52:46Z |
| format | Article |
| id | doaj.art-409e682627024aa0895d3ce807682452 |
| institution | Directory Open Access Journal |
| issn | 2056-5968 |
| language | English |
| last_indexed | 2024-03-13T04:52:46Z |
| publishDate | 2023-06-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Discovery |
| spelling | doaj.art-409e682627024aa0895d3ce8076824522023-06-18T11:06:18ZengNature Publishing GroupCell Discovery2056-59682023-06-019111310.1038/s41421-023-00557-9A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunityXing-Yao Huang0Qi Chen1Meng-Xu Sun2Hang-Yu Zhou3Qing Ye4Wu Chen5Jin-Yu Peng6Yi-Ni Qi7Jun-Qiong Zhai8Ying Tian9Zi-Xin Liu10Yi-Jiao Huang11Yong-Qiang Deng12Xiao-Feng Li13Aiping Wu14Xiao Yang15Guan Yang16Yongyi Shen17Cheng-Feng Qin18State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSInstitute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical CollegeState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSGuangzhou Zoo & Guangzhou Wildlife Research CenterState Key Laboratory for Animal Disease Control and Prevention, Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural UniversityState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of LifeomicsGuangzhou Zoo & Guangzhou Wildlife Research CenterState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSInstitute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical CollegeState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of LifeomicsState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of LifeomicsState Key Laboratory for Animal Disease Control and Prevention, Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural UniversityState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMSAbstract Virus spillover remains a major challenge to public health. A panel of SARS-CoV-2-related coronaviruses have been identified in pangolins, while the infectivity and pathogenicity of these pangolin-origin coronaviruses (pCoV) in humans remain largely unknown. Herein, we comprehensively characterized the infectivity and pathogenicity of a recent pCoV isolate (pCoV-GD01) in human cells and human tracheal epithelium organoids and established animal models in comparison with SARS-CoV-2. pCoV-GD01 showed similar infectivity to SARS-CoV-2 in human cells and organoids. Remarkably, intranasal inoculation of pCoV-GD01 caused severe lung pathological damage in hACE2 mice and could transmit among cocaged hamsters. Interestingly, in vitro neutralization assays and animal heterologous challenge experiments demonstrated that preexisting immunity induced by SARS-CoV-2 infection or vaccination was sufficient to provide at least partial cross-protection against pCoV-GD01 challenge. Our results provide direct evidence supporting pCoV-GD01 as a potential human pathogen and highlight the potential spillover risk.https://doi.org/10.1038/s41421-023-00557-9 |
| spellingShingle | Xing-Yao Huang Qi Chen Meng-Xu Sun Hang-Yu Zhou Qing Ye Wu Chen Jin-Yu Peng Yi-Ni Qi Jun-Qiong Zhai Ying Tian Zi-Xin Liu Yi-Jiao Huang Yong-Qiang Deng Xiao-Feng Li Aiping Wu Xiao Yang Guan Yang Yongyi Shen Cheng-Feng Qin A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity Cell Discovery |
| title | A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity |
| title_full | A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity |
| title_fullStr | A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity |
| title_full_unstemmed | A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity |
| title_short | A pangolin-origin SARS-CoV-2-related coronavirus: infectivity, pathogenicity, and cross-protection by preexisting immunity |
| title_sort | pangolin origin sars cov 2 related coronavirus infectivity pathogenicity and cross protection by preexisting immunity |
| url | https://doi.org/10.1038/s41421-023-00557-9 |
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