Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans
In neurons, microtubules (MTs) span the length of both axons and dendrites, and the molecular motors use these intracellular ‘highways' to transport diverse cargo to the appropriate subcellular locations. Whereas axonal MTs are organized such that the plus-end is oriented out from the cell body...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2013-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/00133 |
| _version_ | 1829097678522286080 |
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| author | Jing Yan Dan L Chao Shiori Toba Kotaro Koyasako Takuo Yasunaga Shinji Hirotsune Kang Shen |
| author_facet | Jing Yan Dan L Chao Shiori Toba Kotaro Koyasako Takuo Yasunaga Shinji Hirotsune Kang Shen |
| author_sort | Jing Yan |
| collection | DOAJ |
| description | In neurons, microtubules (MTs) span the length of both axons and dendrites, and the molecular motors use these intracellular ‘highways' to transport diverse cargo to the appropriate subcellular locations. Whereas axonal MTs are organized such that the plus-end is oriented out from the cell body, dendrites exhibit a mixed MTs polarity containing both minus-end-out and plus-end-out MTs. The molecular mechanisms underlying this differential organization, as well as its functional significance, are unknown. Here, we show that kinesin-1 is critical in establishing the characteristic minus-end-out MT organization of the dendrite in vivo. In unc-116 (kinesin-1/kinesin heavy chain) mutants, the dendritic MTs adopt an axonal-like plus-end-out organization. Kinesin-1 protein is able to cross-link anti-paralleled MTs in vitro. We propose that kinesin-1 regulates the dendrite MT polarity through directly gliding the plus-end-out MTs out of the dendrite using both the motor domain and the C-terminal MT-binding domain. |
| first_indexed | 2024-04-11T09:17:56Z |
| format | Article |
| id | doaj.art-409f7aa436be40fda996868a0a68c662 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:17:56Z |
| publishDate | 2013-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-409f7aa436be40fda996868a0a68c6622022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2013-03-01210.7554/eLife.00133Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegansJing Yan0Dan L Chao1Shiori Toba2Kotaro Koyasako3Takuo Yasunaga4Shinji Hirotsune5Kang Shen6Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Genetic Disease Research, Graduate School of Medicine, Osaka City University, Osaka, JapanDepartment of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan; JST-SENTAN, Japan Science and Technology Agency, Kawaguchi, JapanDepartment of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan; JST-SENTAN, Japan Science and Technology Agency, Kawaguchi, Japan; JST-CREST, Japan Science and Technology Agency, Kawaguchi, JapanDepartment of Genetic Disease Research, Graduate School of Medicine, Osaka City University, Osaka, JapanDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesIn neurons, microtubules (MTs) span the length of both axons and dendrites, and the molecular motors use these intracellular ‘highways' to transport diverse cargo to the appropriate subcellular locations. Whereas axonal MTs are organized such that the plus-end is oriented out from the cell body, dendrites exhibit a mixed MTs polarity containing both minus-end-out and plus-end-out MTs. The molecular mechanisms underlying this differential organization, as well as its functional significance, are unknown. Here, we show that kinesin-1 is critical in establishing the characteristic minus-end-out MT organization of the dendrite in vivo. In unc-116 (kinesin-1/kinesin heavy chain) mutants, the dendritic MTs adopt an axonal-like plus-end-out organization. Kinesin-1 protein is able to cross-link anti-paralleled MTs in vitro. We propose that kinesin-1 regulates the dendrite MT polarity through directly gliding the plus-end-out MTs out of the dendrite using both the motor domain and the C-terminal MT-binding domain.https://elifesciences.org/articles/00133polaritycytoskeletonkinesin-1 |
| spellingShingle | Jing Yan Dan L Chao Shiori Toba Kotaro Koyasako Takuo Yasunaga Shinji Hirotsune Kang Shen Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans eLife polarity cytoskeleton kinesin-1 |
| title | Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans |
| title_full | Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans |
| title_fullStr | Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans |
| title_full_unstemmed | Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans |
| title_short | Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans |
| title_sort | kinesin 1 regulates dendrite microtubule polarity in caenorhabditis elegans |
| topic | polarity cytoskeleton kinesin-1 |
| url | https://elifesciences.org/articles/00133 |
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