Glibenclamide Prevents Diabetes in NOD Mice.
Previous work has revealed that Cx36, the sole connexin expressed in the insulin-producing beta cells, enhances the secretion of insulin, and promotes the resistance of beta cells against pro-inflammatory cytokines. In parallel, the anti-diabetic sulphonylurea glibenclamide was shown to promote the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5178991?pdf=render |
| _version_ | 1818290027666341888 |
|---|---|
| author | Smaragda Lamprianou Conny Gysemans Joanna Bou Saab Helena Pontes Chantal Mathieu Paolo Meda |
| author_facet | Smaragda Lamprianou Conny Gysemans Joanna Bou Saab Helena Pontes Chantal Mathieu Paolo Meda |
| author_sort | Smaragda Lamprianou |
| collection | DOAJ |
| description | Previous work has revealed that Cx36, the sole connexin expressed in the insulin-producing beta cells, enhances the secretion of insulin, and promotes the resistance of beta cells against pro-inflammatory cytokines. In parallel, the anti-diabetic sulphonylurea glibenclamide was shown to promote the assembly and function of Cx36 channels. Here, we assessed whether glibenclamide could protect the insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes. We found that the drug 1) protected in vitro the mouse MIN6 cells from the apoptosis and loss of Cx36, which are induced by Th1 cytokines; 2) prevented the development of hyperglycemia as well as the loss of beta cells and Cx36, which rapidly develop with aging in untreated NOD mice; 3) modified the proportion of effector CD4+ and CD8+ T cells in pancreatic draining lymph nodes. The data imply that an early glibenclamide treatment may help protecting beta cells against the autoimmune attack, which triggers the development of type 1 diabetes. |
| first_indexed | 2024-12-13T02:21:39Z |
| format | Article |
| id | doaj.art-40a570168bd34dbc9aada20240e94a56 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T02:21:39Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-40a570168bd34dbc9aada20240e94a562022-12-22T00:02:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016883910.1371/journal.pone.0168839Glibenclamide Prevents Diabetes in NOD Mice.Smaragda LamprianouConny GysemansJoanna Bou SaabHelena PontesChantal MathieuPaolo MedaPrevious work has revealed that Cx36, the sole connexin expressed in the insulin-producing beta cells, enhances the secretion of insulin, and promotes the resistance of beta cells against pro-inflammatory cytokines. In parallel, the anti-diabetic sulphonylurea glibenclamide was shown to promote the assembly and function of Cx36 channels. Here, we assessed whether glibenclamide could protect the insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes. We found that the drug 1) protected in vitro the mouse MIN6 cells from the apoptosis and loss of Cx36, which are induced by Th1 cytokines; 2) prevented the development of hyperglycemia as well as the loss of beta cells and Cx36, which rapidly develop with aging in untreated NOD mice; 3) modified the proportion of effector CD4+ and CD8+ T cells in pancreatic draining lymph nodes. The data imply that an early glibenclamide treatment may help protecting beta cells against the autoimmune attack, which triggers the development of type 1 diabetes.http://europepmc.org/articles/PMC5178991?pdf=render |
| spellingShingle | Smaragda Lamprianou Conny Gysemans Joanna Bou Saab Helena Pontes Chantal Mathieu Paolo Meda Glibenclamide Prevents Diabetes in NOD Mice. PLoS ONE |
| title | Glibenclamide Prevents Diabetes in NOD Mice. |
| title_full | Glibenclamide Prevents Diabetes in NOD Mice. |
| title_fullStr | Glibenclamide Prevents Diabetes in NOD Mice. |
| title_full_unstemmed | Glibenclamide Prevents Diabetes in NOD Mice. |
| title_short | Glibenclamide Prevents Diabetes in NOD Mice. |
| title_sort | glibenclamide prevents diabetes in nod mice |
| url | http://europepmc.org/articles/PMC5178991?pdf=render |
| work_keys_str_mv | AT smaragdalamprianou glibenclamidepreventsdiabetesinnodmice AT connygysemans glibenclamidepreventsdiabetesinnodmice AT joannabousaab glibenclamidepreventsdiabetesinnodmice AT helenapontes glibenclamidepreventsdiabetesinnodmice AT chantalmathieu glibenclamidepreventsdiabetesinnodmice AT paolomeda glibenclamidepreventsdiabetesinnodmice |