Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis
Streptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. Culturing and environmental factors causing structural differences in mutans were specified. All of the obtained biopolyme...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/10/11800 |
| _version_ | 1818471962304839680 |
|---|---|
| author | Katarzyna Próchniak Małgorzata Pleszczyńska Adrian Wiater Janusz Szczodrak |
| author_facet | Katarzyna Próchniak Małgorzata Pleszczyńska Adrian Wiater Janusz Szczodrak |
| author_sort | Katarzyna Próchniak |
| collection | DOAJ |
| description | Streptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. Culturing and environmental factors causing structural differences in mutans were specified. All of the obtained biopolymers (76 samples) were water-insoluble and most of them (72) had a structure with a predominance of α-(1→3)-linked glucose (i.e., the content of α-(1→3)-linkages in the glucan was always higher than 50%, but did not exceed 76%). An exception were four glucans containing more than 50% of α-(1→6)-sequences. In these structurally unique mutans, the ratio of α-(1→3)- to α-(1→6)-bonds ranged from 0.75 to 0.97. Aside from one polymer, all others had a heavily branched structures and differed in the number of α-(1→3), α-(1→6), and α-(1→3,6) linkages and their mutual proportion. The induction of mutanase production in shaken flask cultures of Trichoderma harzianum by the structurally diverse mutans resulted in enzyme activities ranging from 0.144 to 1.051 U/mL. No statistical correlation was found between the total percentage content of α-(1→3)-linkages in the α-glucan and mutanase activity. Thus, despite biosynthetic differences causing structural variation in the mutans, it did not matter which mutan structures were used to induce mutanase production. |
| first_indexed | 2024-04-14T03:58:57Z |
| format | Article |
| id | doaj.art-40a691edafe844ccbd295bc3d7686c4a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T03:58:57Z |
| publishDate | 2012-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-40a691edafe844ccbd295bc3d7686c4a2022-12-22T02:13:37ZengMDPI AGMolecules1420-30492012-10-011710118001181510.3390/molecules171011800Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase SynthesisKatarzyna PróchniakMałgorzata PleszczyńskaAdrian WiaterJanusz SzczodrakStreptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. Culturing and environmental factors causing structural differences in mutans were specified. All of the obtained biopolymers (76 samples) were water-insoluble and most of them (72) had a structure with a predominance of α-(1→3)-linked glucose (i.e., the content of α-(1→3)-linkages in the glucan was always higher than 50%, but did not exceed 76%). An exception were four glucans containing more than 50% of α-(1→6)-sequences. In these structurally unique mutans, the ratio of α-(1→3)- to α-(1→6)-bonds ranged from 0.75 to 0.97. Aside from one polymer, all others had a heavily branched structures and differed in the number of α-(1→3), α-(1→6), and α-(1→3,6) linkages and their mutual proportion. The induction of mutanase production in shaken flask cultures of Trichoderma harzianum by the structurally diverse mutans resulted in enzyme activities ranging from 0.144 to 1.051 U/mL. No statistical correlation was found between the total percentage content of α-(1→3)-linkages in the α-glucan and mutanase activity. Thus, despite biosynthetic differences causing structural variation in the mutans, it did not matter which mutan structures were used to induce mutanase production.http://www.mdpi.com/1420-3049/17/10/11800mutanglucosyltransferasecariogenic streptococciNMR spectroscopy |
| spellingShingle | Katarzyna Próchniak Małgorzata Pleszczyńska Adrian Wiater Janusz Szczodrak Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis Molecules mutan glucosyltransferase cariogenic streptococci NMR spectroscopy |
| title | Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis |
| title_full | Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis |
| title_fullStr | Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis |
| title_full_unstemmed | Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis |
| title_short | Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis |
| title_sort | structural diversity of streptococcal mutans synthesized under different culture and environmental conditions and its effect on mutanase synthesis |
| topic | mutan glucosyltransferase cariogenic streptococci NMR spectroscopy |
| url | http://www.mdpi.com/1420-3049/17/10/11800 |
| work_keys_str_mv | AT katarzynaprochniak structuraldiversityofstreptococcalmutanssynthesizedunderdifferentcultureandenvironmentalconditionsanditseffectonmutanasesynthesis AT małgorzatapleszczynska structuraldiversityofstreptococcalmutanssynthesizedunderdifferentcultureandenvironmentalconditionsanditseffectonmutanasesynthesis AT adrianwiater structuraldiversityofstreptococcalmutanssynthesizedunderdifferentcultureandenvironmentalconditionsanditseffectonmutanasesynthesis AT januszszczodrak structuraldiversityofstreptococcalmutanssynthesizedunderdifferentcultureandenvironmentalconditionsanditseffectonmutanasesynthesis |