Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis
Korean pine broadleaf mixed forest is an important ecosystem for maintaining biodiversity in Northeast China. Korean pine is also an important species for the production of timber and nuts in the mountainous areas of Northeast China. In this study, we compared three types of Korean pine callus and f...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/5/577 |
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| author | Chunxue Peng Fang Gao Hao Wang Hailong Shen Ling Yang |
| author_facet | Chunxue Peng Fang Gao Hao Wang Hailong Shen Ling Yang |
| author_sort | Chunxue Peng |
| collection | DOAJ |
| description | Korean pine broadleaf mixed forest is an important ecosystem for maintaining biodiversity in Northeast China. Korean pine is also an important species for the production of timber and nuts in the mountainous areas of Northeast China. In this study, we compared three types of Korean pine callus and found that embryogenic callus had high amounts of storage substances (protein, sugar and starch). Non-embryonic callus had high levels of polyphenols and polyphenol oxidation, while callus that lost somatic embryogenesis potential had lower levels of storage substances (protein, sugar and starch) and higher contents of peroxidase and catalase. These results indicate that high contents of storage substances (protein, sugar and starch), and low levels of polyphenols and polyphenol oxidase can be used as physiological markers of callus with somatic embryogenic potential. During the development process of Korean pine somatic embryos, fresh weight and dry weight gradually increased, while water content gradually decreased. Soluble protein, starch, soluble sugar and superoxide dismutase also increased during development, while peroxidase and catalase levels reduced over time. These results indicate that somatic embryogenesis involves energy storage, and antioxidant enzymes cooperate to regulate the occurrence and development of embryos. These results provide physiological markers for identification of embryogenic callus with somatic embryogenesis, to evaluate callus suitable for somatic embryogenesis, and provide basis for further research on the molecular mechanisms of somatic embryogenesis. |
| first_indexed | 2024-03-10T19:40:32Z |
| format | Article |
| id | doaj.art-40a7e2dade3446938393eeb9fb3e36d7 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T19:40:32Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-40a7e2dade3446938393eeb9fb3e36d72023-11-20T01:14:47ZengMDPI AGForests1999-49072020-05-0111557710.3390/f11050577Physiological and Biochemical Traits in Korean Pine Somatic EmbryogenesisChunxue Peng0Fang Gao1Hao Wang2Hailong Shen3Ling Yang4State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaKorean pine broadleaf mixed forest is an important ecosystem for maintaining biodiversity in Northeast China. Korean pine is also an important species for the production of timber and nuts in the mountainous areas of Northeast China. In this study, we compared three types of Korean pine callus and found that embryogenic callus had high amounts of storage substances (protein, sugar and starch). Non-embryonic callus had high levels of polyphenols and polyphenol oxidation, while callus that lost somatic embryogenesis potential had lower levels of storage substances (protein, sugar and starch) and higher contents of peroxidase and catalase. These results indicate that high contents of storage substances (protein, sugar and starch), and low levels of polyphenols and polyphenol oxidase can be used as physiological markers of callus with somatic embryogenic potential. During the development process of Korean pine somatic embryos, fresh weight and dry weight gradually increased, while water content gradually decreased. Soluble protein, starch, soluble sugar and superoxide dismutase also increased during development, while peroxidase and catalase levels reduced over time. These results indicate that somatic embryogenesis involves energy storage, and antioxidant enzymes cooperate to regulate the occurrence and development of embryos. These results provide physiological markers for identification of embryogenic callus with somatic embryogenesis, to evaluate callus suitable for somatic embryogenesis, and provide basis for further research on the molecular mechanisms of somatic embryogenesis.https://www.mdpi.com/1999-4907/11/5/577<i>Pinus koraiensis</i> Sieb. et Zucc.somatic embryogenesisnon-embryogenic callusembryogenic callusresistance oxidase activity |
| spellingShingle | Chunxue Peng Fang Gao Hao Wang Hailong Shen Ling Yang Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis Forests <i>Pinus koraiensis</i> Sieb. et Zucc. somatic embryogenesis non-embryogenic callus embryogenic callus resistance oxidase activity |
| title | Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis |
| title_full | Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis |
| title_fullStr | Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis |
| title_full_unstemmed | Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis |
| title_short | Physiological and Biochemical Traits in Korean Pine Somatic Embryogenesis |
| title_sort | physiological and biochemical traits in korean pine somatic embryogenesis |
| topic | <i>Pinus koraiensis</i> Sieb. et Zucc. somatic embryogenesis non-embryogenic callus embryogenic callus resistance oxidase activity |
| url | https://www.mdpi.com/1999-4907/11/5/577 |
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