Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool
Background: Cryptosporidiosis, a diarrheal disease caused by the protozoan parasite Cryptosporidium spp. has become recognized as one of the most common causes of water borne diseases in humans. Aims and Objectives: To compare the sensitivity of ELISA and Microscopy for detection of Cryptospori...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5088/9713_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh).pdf |
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| author | Seema Mittal Madhu Sharma Uma Chaudhary Aparna Yadav |
| author_facet | Seema Mittal Madhu Sharma Uma Chaudhary Aparna Yadav |
| author_sort | Seema Mittal |
| collection | DOAJ |
| description | Background: Cryptosporidiosis, a diarrheal disease caused
by the protozoan parasite Cryptosporidium spp. has become
recognized as one of the most common causes of water borne
diseases in humans.
Aims and Objectives: To compare the sensitivity of ELISA and
Microscopy for detection of Cryptosporidium in stool samples.
Materials and Methods: The study was conducted in the
Department of Microbiology of PT. B.D. Sharma PGIMS Rohtak,
between January 2011 to june 2011 on 50 stool samples, which
were processed for detection of cryptosporidial antigen by
ELISA and detection of cysts by microscopy (Modified Ziehl and
Nelsen staining).
Study and Design: This was a prospective study conducted
in the Department of Microbiology in PT. BD Sharma, PGIMS,
Rohtak, India.
Result: Out of total, 50 stool samples eighteen (36%) samples
were found positive for Cryptosporidium cysts by microscopy in
comparison to 3(6%) stool samples which were found positive
for cryptosporidial antigen by ELISA. Samples found positive
with ELISA were also positive with microscopy. Sensitivity,
specificity, positive predictive value and negative predictive
value for ELISA was 16.7%, 100%, 100% and 68% respectively.
Conclusion: The study concludes that stool microscopic
Modified acid fast staining is more sensitive method than
ELISA for detection of Cryptosporidium in stool samples but the
specificity of ELISA was more than microscopy. |
| first_indexed | 2024-12-23T03:03:07Z |
| format | Article |
| id | doaj.art-40aca405dd1b423fa0575d4ae719ecdd |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-23T03:03:07Z |
| publishDate | 2014-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-40aca405dd1b423fa0575d4ae719ecdd2022-12-21T18:02:22ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-11-01811DC07DC0810.7860/JCDR/2014/9713.5088Comparison of ELISA and Microscopy for detection of Cryptosporidium in stoolSeema Mittal0Madhu Sharma1Uma Chaudhary2Aparna Yadav3Senior Resident, Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.Professor, Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.Senior Professor and Head, Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.Professor, Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.Background: Cryptosporidiosis, a diarrheal disease caused by the protozoan parasite Cryptosporidium spp. has become recognized as one of the most common causes of water borne diseases in humans. Aims and Objectives: To compare the sensitivity of ELISA and Microscopy for detection of Cryptosporidium in stool samples. Materials and Methods: The study was conducted in the Department of Microbiology of PT. B.D. Sharma PGIMS Rohtak, between January 2011 to june 2011 on 50 stool samples, which were processed for detection of cryptosporidial antigen by ELISA and detection of cysts by microscopy (Modified Ziehl and Nelsen staining). Study and Design: This was a prospective study conducted in the Department of Microbiology in PT. BD Sharma, PGIMS, Rohtak, India. Result: Out of total, 50 stool samples eighteen (36%) samples were found positive for Cryptosporidium cysts by microscopy in comparison to 3(6%) stool samples which were found positive for cryptosporidial antigen by ELISA. Samples found positive with ELISA were also positive with microscopy. Sensitivity, specificity, positive predictive value and negative predictive value for ELISA was 16.7%, 100%, 100% and 68% respectively. Conclusion: The study concludes that stool microscopic Modified acid fast staining is more sensitive method than ELISA for detection of Cryptosporidium in stool samples but the specificity of ELISA was more than microscopy.https://jcdr.net/articles/PDF/5088/9713_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh).pdfcryptosporidiumelisamicroscopy |
| spellingShingle | Seema Mittal Madhu Sharma Uma Chaudhary Aparna Yadav Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool Journal of Clinical and Diagnostic Research cryptosporidium elisa microscopy |
| title | Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool |
| title_full | Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool |
| title_fullStr | Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool |
| title_full_unstemmed | Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool |
| title_short | Comparison of ELISA and Microscopy for detection of Cryptosporidium in stool |
| title_sort | comparison of elisa and microscopy for detection of cryptosporidium in stool |
| topic | cryptosporidium elisa microscopy |
| url | https://jcdr.net/articles/PDF/5088/9713_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh).pdf |
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