Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.)
This study aimed to investigate the sublethal effects of thiacloprid on microRNA (miRNA) expression in honeybees (Apis mellifera L.) and the role of a specific miRNA, ame-miR-283–5p, in thiacloprid resistance. The high-throughput small RNA-sequencing was used to analyze global miRNA expression profi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323010035 |
| _version_ | 1797676610383511552 |
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| author | Tengfei Shi Xingchuan Jiang Haiqun Cao Linsheng Yu |
| author_facet | Tengfei Shi Xingchuan Jiang Haiqun Cao Linsheng Yu |
| author_sort | Tengfei Shi |
| collection | DOAJ |
| description | This study aimed to investigate the sublethal effects of thiacloprid on microRNA (miRNA) expression in honeybees (Apis mellifera L.) and the role of a specific miRNA, ame-miR-283–5p, in thiacloprid resistance. The high-throughput small RNA-sequencing was used to analyze global miRNA expression profile changes in honeybees orally exposed to sublethal concentrations of thiacloprid (20 mg/L and 4 mg/L) for 72 h. Thiacloprid at 20 mg/L had no observed adverse effects. In addition, bees were fed with miRNA mimics or inhibitors to increase or decrease ame-miR-283–5p expression, and its effects on P450 gene expression (CYP9Q2 and CYP9Q3) were examined. Thiacloprid susceptibility was also detected. The results showed that treatment with thiacloprid at 20 mg/L and 4 mg/L induced 11 and five differentially expressed miRNAs (DEMs), respectively. Bioinformatic analysis suggested that the DEMs are mainly involved in the regulation of growth and development, metabolism, nerve conduction, and behavior. ame-miR-283–5p was downregulated by both concentrations, which was validated using quantitative real-time reverse transcription PCR analysis. Enhancing ame-miR-283–5p expression significantly inhibited CYP9Q2 mRNA and protein expression, and increased thiacloprid toxicity, while reducing ame-miR-283–5p expression significantly promoted CYP9Q2 expression, and decreased thiacloprid susceptibility. Our study revealed a novel role of miRNAs in insecticide resistance in honeybees. |
| first_indexed | 2024-03-11T22:31:42Z |
| format | Article |
| id | doaj.art-40b0c38cf5f1492dace41cd44b42b025 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-11T22:31:42Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-40b0c38cf5f1492dace41cd44b42b0252023-09-23T05:10:13ZengElsevierEcotoxicology and Environmental Safety0147-65132023-10-01264115499Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.)Tengfei Shi0Xingchuan Jiang1Haiqun Cao2Linsheng Yu3Corresponding authors.; School of Plant Protection, Institute of Apiculture Research, Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaSchool of Plant Protection, Institute of Apiculture Research, Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaSchool of Plant Protection, Institute of Apiculture Research, Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaCorresponding authors.; School of Plant Protection, Institute of Apiculture Research, Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei 230036, ChinaThis study aimed to investigate the sublethal effects of thiacloprid on microRNA (miRNA) expression in honeybees (Apis mellifera L.) and the role of a specific miRNA, ame-miR-283–5p, in thiacloprid resistance. The high-throughput small RNA-sequencing was used to analyze global miRNA expression profile changes in honeybees orally exposed to sublethal concentrations of thiacloprid (20 mg/L and 4 mg/L) for 72 h. Thiacloprid at 20 mg/L had no observed adverse effects. In addition, bees were fed with miRNA mimics or inhibitors to increase or decrease ame-miR-283–5p expression, and its effects on P450 gene expression (CYP9Q2 and CYP9Q3) were examined. Thiacloprid susceptibility was also detected. The results showed that treatment with thiacloprid at 20 mg/L and 4 mg/L induced 11 and five differentially expressed miRNAs (DEMs), respectively. Bioinformatic analysis suggested that the DEMs are mainly involved in the regulation of growth and development, metabolism, nerve conduction, and behavior. ame-miR-283–5p was downregulated by both concentrations, which was validated using quantitative real-time reverse transcription PCR analysis. Enhancing ame-miR-283–5p expression significantly inhibited CYP9Q2 mRNA and protein expression, and increased thiacloprid toxicity, while reducing ame-miR-283–5p expression significantly promoted CYP9Q2 expression, and decreased thiacloprid susceptibility. Our study revealed a novel role of miRNAs in insecticide resistance in honeybees.http://www.sciencedirect.com/science/article/pii/S0147651323010035Apis mellifera L.ThiaclopridSublethal effectsAme-miR-283–5p expressionInsecticides resistance |
| spellingShingle | Tengfei Shi Xingchuan Jiang Haiqun Cao Linsheng Yu Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) Ecotoxicology and Environmental Safety Apis mellifera L. Thiacloprid Sublethal effects Ame-miR-283–5p expression Insecticides resistance |
| title | Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) |
| title_full | Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) |
| title_fullStr | Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) |
| title_full_unstemmed | Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) |
| title_short | Exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of microRNAs in honeybees (Apis mellifera L.) |
| title_sort | exposure to sublethal concentrations of thiacloprid insecticide modulated the expression of micrornas in honeybees apis mellifera l |
| topic | Apis mellifera L. Thiacloprid Sublethal effects Ame-miR-283–5p expression Insecticides resistance |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323010035 |
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