Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis
<p>Abstract</p> <p>Background</p> <p><it>Lactobacillus reuteri </it>harbors the genes responsible for glycerol utilization and vitamin B<sub>12 </sub>synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this...
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/10/1/55 |
| _version_ | 1818060425385738240 |
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| author | Saulnier Delphine MA Spinler Jennifer K Santos Filipe Molenaar Douwe Teusink Bas de Vos Willem M Versalovic James Hugenholtz Jeroen |
| author_facet | Saulnier Delphine MA Spinler Jennifer K Santos Filipe Molenaar Douwe Teusink Bas de Vos Willem M Versalovic James Hugenholtz Jeroen |
| author_sort | Saulnier Delphine MA |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Lactobacillus reuteri </it>harbors the genes responsible for glycerol utilization and vitamin B<sub>12 </sub>synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene (<it>lreu_1750</it>) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.</p> <p>Results</p> <p>In this contribution, we have overexpressed and inactivated the gene encoding the putative PocR in <it>L. reuteri</it>. The comparison of these strains provided metabolic and transcriptional evidence that this regulatory protein controls the expression of the operons encoding glycerol utilization and vitamin B<sub>12 </sub>synthesis.</p> <p>Conclusions</p> <p>We provide clear experimental evidence for assigning Lreu_1750 as PocR in <it>Lactobacillus reuteri</it>. Our genome-wide transcriptional analysis further identifies the loci contained in the PocR regulon. The findings reported here could be used to improve the production-yield of vitamin B<sub>12</sub>, 1,3-propanediol and reuterin, all industrially relevant compounds.</p> |
| first_indexed | 2024-12-10T13:32:13Z |
| format | Article |
| id | doaj.art-40b45050a7f44d9287146f07d69d126b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-10T13:32:13Z |
| publishDate | 2011-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-40b45050a7f44d9287146f07d69d126b2022-12-22T01:46:56ZengBMCMicrobial Cell Factories1475-28592011-07-011015510.1186/1475-2859-10-55Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesisSaulnier Delphine MASpinler Jennifer KSantos FilipeMolenaar DouweTeusink Basde Vos Willem MVersalovic JamesHugenholtz Jeroen<p>Abstract</p> <p>Background</p> <p><it>Lactobacillus reuteri </it>harbors the genes responsible for glycerol utilization and vitamin B<sub>12 </sub>synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene (<it>lreu_1750</it>) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.</p> <p>Results</p> <p>In this contribution, we have overexpressed and inactivated the gene encoding the putative PocR in <it>L. reuteri</it>. The comparison of these strains provided metabolic and transcriptional evidence that this regulatory protein controls the expression of the operons encoding glycerol utilization and vitamin B<sub>12 </sub>synthesis.</p> <p>Conclusions</p> <p>We provide clear experimental evidence for assigning Lreu_1750 as PocR in <it>Lactobacillus reuteri</it>. Our genome-wide transcriptional analysis further identifies the loci contained in the PocR regulon. The findings reported here could be used to improve the production-yield of vitamin B<sub>12</sub>, 1,3-propanediol and reuterin, all industrially relevant compounds.</p>http://www.microbialcellfactories.com/content/10/1/55 |
| spellingShingle | Saulnier Delphine MA Spinler Jennifer K Santos Filipe Molenaar Douwe Teusink Bas de Vos Willem M Versalovic James Hugenholtz Jeroen Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis Microbial Cell Factories |
| title | Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis |
| title_full | Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis |
| title_fullStr | Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis |
| title_full_unstemmed | Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis |
| title_short | Functional identification in <it>Lactobacillus reuteri </it>of a PocR-like transcription factor regulating glycerol utilization and vitamin B<sub>12 </sub>synthesis |
| title_sort | functional identification in it lactobacillus reuteri it of a pocr like transcription factor regulating glycerol utilization and vitamin b sub 12 sub synthesis |
| url | http://www.microbialcellfactories.com/content/10/1/55 |
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