Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED
Ultraviolet disinfection is an important method for controlling the large-scale outbreaks of diseases in aquaculture. As a novel and promising light source, ultraviolet light-emitting diode (UV-LED) has the advantages of safety, high efficiency and no environmental pollution risks. However, it remai...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1139713/full |
| _version_ | 1797828923977891840 |
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| author | Weixiao Zhang Weixiao Zhang Ruipin Huang Ruipin Huang Tao Zhang Tao Zhang Baolong Wang Baolong Wang Na Li Na Li Yan Sun Yan Sun He Ma He Ma Qian Zhang Qian Zhang Junxin Zhang Ying Liu Ying Liu |
| author_facet | Weixiao Zhang Weixiao Zhang Ruipin Huang Ruipin Huang Tao Zhang Tao Zhang Baolong Wang Baolong Wang Na Li Na Li Yan Sun Yan Sun He Ma He Ma Qian Zhang Qian Zhang Junxin Zhang Ying Liu Ying Liu |
| author_sort | Weixiao Zhang |
| collection | DOAJ |
| description | Ultraviolet disinfection is an important method for controlling the large-scale outbreaks of diseases in aquaculture. As a novel and promising light source, ultraviolet light-emitting diode (UV-LED) has the advantages of safety, high efficiency and no environmental pollution risks. However, it remains unclear whether UV-LEDs can replace traditional UV light sources for aquaculture water treatment processes. Present study aimed to investigate the efficacy of UVC-LEDs (265 nm) on pathogenic bacteria, specifically Aeromonas salmonicida and Escherichia coli. The effects of UVC-LED dose, light conditions, and temperature on bacterial reactivation were also investigated. The results showed that exposure to UVC-LED effectively inactivated both types of bacteria. To achieve 4.5-log inactivation of A. salmonicida and E. coli, 24 mJ/cm2 and 28 mJ/cm2 UVC-LED irradiation were required, and the inactivation rate increased with increasing UVC-LED fluence. Both A. salmonicida and E. coli were revived after UVC-LED disinfection, and photoreactivation was significantly higher than dark reactivation. Bacterial reactivation rate due to high-dose UVC-LED treatment was significantly lower than that of low-dose. After 72 h of reactivation, photoreactivation and dark reactivation rates were 1 ± 0.4% and 2.2 ± 0.2%for A. salmonicida, and 0.02% and 0% for E. coli, respectively. Besides, the photoreactivation rates for the two bacteria exhibited different correlations with temperature. The highest photoreactivation rate for A. salmonicida was 68.7 ± 4% at 20°C, while the highest photoreactivation rate for E. coli was 53.98 ± 2.9% at 15°C for 48 h. This study reveals the rapid and efficient inactivation of bacteria by UVC-LED, and elucidates the mechanism and influencing factors for inactivation and reactivation by UVC-LED. The study also highlights that adequate UVC-LED irradiation and avoidance of visible light after UVC-LED disinfection can effectively inhibit bacterial reactivation. Our findings form a reference for the design and operation of UV disinfection in aquaculture. |
| first_indexed | 2024-04-09T13:12:12Z |
| format | Article |
| id | doaj.art-40b49518962f4a4fabc0941115430000 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-09T13:12:12Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-40b49518962f4a4fabc09411154300002023-05-12T06:19:48ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-05-011010.3389/fmars.2023.11397131139713Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LEDWeixiao Zhang0Weixiao Zhang1Ruipin Huang2Ruipin Huang3Tao Zhang4Tao Zhang5Baolong Wang6Baolong Wang7Na Li8Na Li9Yan Sun10Yan Sun11He Ma12He Ma13Qian Zhang14Qian Zhang15Junxin Zhang16Ying Liu17Ying Liu18College of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian, ChinaKey Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, ChinaUltraviolet disinfection is an important method for controlling the large-scale outbreaks of diseases in aquaculture. As a novel and promising light source, ultraviolet light-emitting diode (UV-LED) has the advantages of safety, high efficiency and no environmental pollution risks. However, it remains unclear whether UV-LEDs can replace traditional UV light sources for aquaculture water treatment processes. Present study aimed to investigate the efficacy of UVC-LEDs (265 nm) on pathogenic bacteria, specifically Aeromonas salmonicida and Escherichia coli. The effects of UVC-LED dose, light conditions, and temperature on bacterial reactivation were also investigated. The results showed that exposure to UVC-LED effectively inactivated both types of bacteria. To achieve 4.5-log inactivation of A. salmonicida and E. coli, 24 mJ/cm2 and 28 mJ/cm2 UVC-LED irradiation were required, and the inactivation rate increased with increasing UVC-LED fluence. Both A. salmonicida and E. coli were revived after UVC-LED disinfection, and photoreactivation was significantly higher than dark reactivation. Bacterial reactivation rate due to high-dose UVC-LED treatment was significantly lower than that of low-dose. After 72 h of reactivation, photoreactivation and dark reactivation rates were 1 ± 0.4% and 2.2 ± 0.2%for A. salmonicida, and 0.02% and 0% for E. coli, respectively. Besides, the photoreactivation rates for the two bacteria exhibited different correlations with temperature. The highest photoreactivation rate for A. salmonicida was 68.7 ± 4% at 20°C, while the highest photoreactivation rate for E. coli was 53.98 ± 2.9% at 15°C for 48 h. This study reveals the rapid and efficient inactivation of bacteria by UVC-LED, and elucidates the mechanism and influencing factors for inactivation and reactivation by UVC-LED. The study also highlights that adequate UVC-LED irradiation and avoidance of visible light after UVC-LED disinfection can effectively inhibit bacterial reactivation. Our findings form a reference for the design and operation of UV disinfection in aquaculture.https://www.frontiersin.org/articles/10.3389/fmars.2023.1139713/fullUVC-LEDaquaculturebacteria disinfectionphotoreactivationdark repair |
| spellingShingle | Weixiao Zhang Weixiao Zhang Ruipin Huang Ruipin Huang Tao Zhang Tao Zhang Baolong Wang Baolong Wang Na Li Na Li Yan Sun Yan Sun He Ma He Ma Qian Zhang Qian Zhang Junxin Zhang Ying Liu Ying Liu Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED Frontiers in Marine Science UVC-LED aquaculture bacteria disinfection photoreactivation dark repair |
| title | Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED |
| title_full | Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED |
| title_fullStr | Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED |
| title_full_unstemmed | Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED |
| title_short | Study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by UVC-LED |
| title_sort | study on the inactivation and reactivation mechanism of pathogenic bacteria in aquaculture by uvc led |
| topic | UVC-LED aquaculture bacteria disinfection photoreactivation dark repair |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1139713/full |
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