Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia
Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side...
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MDPI AG
2018-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/10/2638 |
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| author | Bo-Ram Jin Hyo-Jung Kim Sang-Kyun Park Myoung-Seok Kim Kwang-Ho Lee Il-Joo Yoon Hyo-Jin An |
| author_facet | Bo-Ram Jin Hyo-Jung Kim Sang-Kyun Park Myoung-Seok Kim Kwang-Ho Lee Il-Joo Yoon Hyo-Jin An |
| author_sort | Bo-Ram Jin |
| collection | DOAJ |
| description | Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments. |
| first_indexed | 2024-12-12T07:09:45Z |
| format | Article |
| id | doaj.art-40b61de090074957b27792b55f97400b |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T07:09:45Z |
| publishDate | 2018-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-40b61de090074957b27792b55f97400b2022-12-22T00:33:39ZengMDPI AGMolecules1420-30492018-10-012310263810.3390/molecules23102638molecules23102638Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic HyperplasiaBo-Ram Jin0Hyo-Jung Kim1Sang-Kyun Park2Myoung-Seok Kim3Kwang-Ho Lee4Il-Joo Yoon5Hyo-Jin An6Department of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, KoreaDepartment of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, KoreaDepartment of Meridian & Acupoint, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, KoreaCentral Research Institue of Hawon Pharmaceutical, Jangheung 59338, KoreaCentral Research Institue of Hawon Pharmaceutical, Jangheung 59338, KoreaCentral Research Institue of Hawon Pharmaceutical, Jangheung 59338, KoreaDepartment of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, KoreaBenign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments.http://www.mdpi.com/1420-3049/23/10/2638BPHHBX-5proliferationinflammationRWPE-1WPMY-1testosterone-induced BPH rat model |
| spellingShingle | Bo-Ram Jin Hyo-Jung Kim Sang-Kyun Park Myoung-Seok Kim Kwang-Ho Lee Il-Joo Yoon Hyo-Jin An Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia Molecules BPH HBX-5 proliferation inflammation RWPE-1 WPMY-1 testosterone-induced BPH rat model |
| title | Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia |
| title_full | Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia |
| title_fullStr | Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia |
| title_full_unstemmed | Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia |
| title_short | Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia |
| title_sort | anti proliferative effects of hbx 5 on progression of benign prostatic hyperplasia |
| topic | BPH HBX-5 proliferation inflammation RWPE-1 WPMY-1 testosterone-induced BPH rat model |
| url | http://www.mdpi.com/1420-3049/23/10/2638 |
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