Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy
Rhabdoid tumor is a very aggressive and hardly curable pediatric malignancy. It commonly starts in the kidneys but also can occur in the brain, liver, and other organs. The treatment of this tumor usually involves a combination of surgery, radiation, and chemotherapy. Because this tumor is rare, the...
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| Format: | Article |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmed.2019.00187/full |
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| author | Elizaveta Fasler-Kan Elizaveta Fasler-Kan Elizaveta Fasler-Kan Nijas Aliu Frank-Martin Haecker Frank-Martin Haecker Natalia Maltsev Sabrina Ruggiero Sabrina Ruggiero Dietmar Cholewa Dietmar Cholewa Andreas Bartenstein Andreas Bartenstein Milan Milošević Milan Milošević Steffen M. Berger Steffen M. Berger |
| author_facet | Elizaveta Fasler-Kan Elizaveta Fasler-Kan Elizaveta Fasler-Kan Nijas Aliu Frank-Martin Haecker Frank-Martin Haecker Natalia Maltsev Sabrina Ruggiero Sabrina Ruggiero Dietmar Cholewa Dietmar Cholewa Andreas Bartenstein Andreas Bartenstein Milan Milošević Milan Milošević Steffen M. Berger Steffen M. Berger |
| author_sort | Elizaveta Fasler-Kan |
| collection | DOAJ |
| description | Rhabdoid tumor is a very aggressive and hardly curable pediatric malignancy. It commonly starts in the kidneys but also can occur in the brain, liver, and other organs. The treatment of this tumor usually involves a combination of surgery, radiation, and chemotherapy. Because this tumor is rare, there is still limited experience with a defined standard of care. Cytogenetic analysis is an important routine method to monitor chromosomal aberrations. We have analyzed metaphases of the G-401 rhabdoid tumor cell line. In these cells we have observed metaphases with derivative chromosome 12 arising from partial trisomy 7p. With increasing passage number the numbers of metaphases having this derivative chromosome 12 were found to be higher. In passage number 2 only one metaphase had this pathological chromosome 12. By passage number 10 and passage number 15 about 25 and 95% of this derivative chromosome 12 were found, respectively. We were able to subclone G-401 cells by limiting dilutions and successfully separated cells having apparently normal karyotypes from cells having derivative chromosome 12. Using the cell proliferation assay we showed that clones possessing the derivative chromosome 12 grew more rapidly than clones with normal chromosomes. The cell cycle analysis confirmed this observation. Overall, in this study we describe for the first time a 7p triplication in a rare rhabdoid tumor of kidney. Both types of clones described in this study could be used as a preclinical model to study the involvement of partial chromosome 7 alterations in the development of rhabdoid tumors. |
| first_indexed | 2024-12-12T23:48:13Z |
| format | Article |
| id | doaj.art-40b7232bc3ef453b92198f5819937236 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-12T23:48:13Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-40b7232bc3ef453b92198f58199372362022-12-22T00:06:47ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2019-08-01610.3389/fmed.2019.00187466842Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p TrisomyElizaveta Fasler-Kan0Elizaveta Fasler-Kan1Elizaveta Fasler-Kan2Nijas Aliu3Frank-Martin Haecker4Frank-Martin Haecker5Natalia Maltsev6Sabrina Ruggiero7Sabrina Ruggiero8Dietmar Cholewa9Dietmar Cholewa10Andreas Bartenstein11Andreas Bartenstein12Milan Milošević13Milan Milošević14Steffen M. Berger15Steffen M. Berger16Department of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Biomedicine, University of Basel, University Hospital Basel, Basel, SwitzerlandDepartment of Human Genetics, University Children's Hospital, Inselspital, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital of Eastern Switzerland, St. Gallen, SwitzerlandFaculty of Medicine, University of Basel, Basel, SwitzerlandDepartment of Human Genetics and USA Computation Institute, University of Chicago, Chicago, IL, United StatesDepartment of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital, University of Bern, Bern, SwitzerlandDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandRhabdoid tumor is a very aggressive and hardly curable pediatric malignancy. It commonly starts in the kidneys but also can occur in the brain, liver, and other organs. The treatment of this tumor usually involves a combination of surgery, radiation, and chemotherapy. Because this tumor is rare, there is still limited experience with a defined standard of care. Cytogenetic analysis is an important routine method to monitor chromosomal aberrations. We have analyzed metaphases of the G-401 rhabdoid tumor cell line. In these cells we have observed metaphases with derivative chromosome 12 arising from partial trisomy 7p. With increasing passage number the numbers of metaphases having this derivative chromosome 12 were found to be higher. In passage number 2 only one metaphase had this pathological chromosome 12. By passage number 10 and passage number 15 about 25 and 95% of this derivative chromosome 12 were found, respectively. We were able to subclone G-401 cells by limiting dilutions and successfully separated cells having apparently normal karyotypes from cells having derivative chromosome 12. Using the cell proliferation assay we showed that clones possessing the derivative chromosome 12 grew more rapidly than clones with normal chromosomes. The cell cycle analysis confirmed this observation. Overall, in this study we describe for the first time a 7p triplication in a rare rhabdoid tumor of kidney. Both types of clones described in this study could be used as a preclinical model to study the involvement of partial chromosome 7 alterations in the development of rhabdoid tumors.https://www.frontiersin.org/article/10.3389/fmed.2019.00187/fullchromosomal aberrationpartial 7p trisomypathophysiologyrhabdoid tumorFISH assayproliferation |
| spellingShingle | Elizaveta Fasler-Kan Elizaveta Fasler-Kan Elizaveta Fasler-Kan Nijas Aliu Frank-Martin Haecker Frank-Martin Haecker Natalia Maltsev Sabrina Ruggiero Sabrina Ruggiero Dietmar Cholewa Dietmar Cholewa Andreas Bartenstein Andreas Bartenstein Milan Milošević Milan Milošević Steffen M. Berger Steffen M. Berger Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy Frontiers in Medicine chromosomal aberration partial 7p trisomy pathophysiology rhabdoid tumor FISH assay proliferation |
| title | Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy |
| title_full | Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy |
| title_fullStr | Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy |
| title_full_unstemmed | Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy |
| title_short | Chromosomal Heterogeneity of the G-401 Rhabdoid Tumor Cell Line: Unusual Partial 7p Trisomy |
| title_sort | chromosomal heterogeneity of the g 401 rhabdoid tumor cell line unusual partial 7p trisomy |
| topic | chromosomal aberration partial 7p trisomy pathophysiology rhabdoid tumor FISH assay proliferation |
| url | https://www.frontiersin.org/article/10.3389/fmed.2019.00187/full |
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