Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
Abstract Background Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the...
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| Format: | Article |
| Language: | English |
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SciELO
2017-04-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s40409-017-0114-y |
| _version_ | 1818321618684870656 |
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| author | David Meléndez-Martínez Juan Manuel Muñoz Guillermo Barraza-Garza Martha Sandra Cruz-Peréz Ana Gatica-Colima Emilio Alvarez-Parrilla Luis Fernando Plenge-Tellechea |
| author_facet | David Meléndez-Martínez Juan Manuel Muñoz Guillermo Barraza-Garza Martha Sandra Cruz-Peréz Ana Gatica-Colima Emilio Alvarez-Parrilla Luis Fernando Plenge-Tellechea |
| author_sort | David Meléndez-Martínez |
| collection | DOAJ |
| description | Abstract Background Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by Crotalus molossus nigrescens venom using erythrocytes. Methods Human erythrocytes were washed and incubated with different Crotalus molossus nigrescens venom concentrations (0–640 μg/mL). After 24 h, the hemolytic activity was measured followed by attenuated total reflectance-Fourier transform infrared spectroscopy, non-denaturing PAGE, conjugated diene and thiobarbituric acid reactive substances determination. Results Low concentrations of venom (<10 μg/mL) generates oxyhemoglobin release by hemolysis, whereas higher concentrations produced a hemoglobin shift of valence, producing methemoglobin (>40 μg/mL). This substance is not degraded by proteases present in the venom. By infrared spectroscopy, starting in 80 μg/mL, we observed changes in bands that are associated with protein damage (1660 and 1540 cm−1) and lipid peroxidation (2960, 2920 and 1740 cm−1). Lipid peroxidation was confirmed by conjugated diene and thiobarbituric acid reactive substance determination, in which differences were observed between the control and erythrocytes treated with venom. Conclusions Crotalus molossus nigrescens venom provokes hemolysis and oxidative stress, which induces methemoglobin formation, loss of protein structure and lipid peroxidation. |
| first_indexed | 2024-12-13T10:43:46Z |
| format | Article |
| id | doaj.art-40b7ce35542c466898d66f5f492f1ba7 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-13T10:43:46Z |
| publishDate | 2017-04-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-40b7ce35542c466898d66f5f492f1ba72022-12-21T23:50:20ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992017-04-012311710.1186/s40409-017-0114-yRattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytesDavid Meléndez-Martínez0Juan Manuel Muñoz1Guillermo Barraza-Garza2Martha Sandra Cruz-Peréz3Ana Gatica-Colima4Emilio Alvarez-Parrilla5Luis Fernando Plenge-Tellechea6Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezDepartamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezDepartamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezHerpetario de la Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Universidad Autónoma de QuerétaroDepartamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezDepartamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezDepartamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad JuárezAbstract Background Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by Crotalus molossus nigrescens venom using erythrocytes. Methods Human erythrocytes were washed and incubated with different Crotalus molossus nigrescens venom concentrations (0–640 μg/mL). After 24 h, the hemolytic activity was measured followed by attenuated total reflectance-Fourier transform infrared spectroscopy, non-denaturing PAGE, conjugated diene and thiobarbituric acid reactive substances determination. Results Low concentrations of venom (<10 μg/mL) generates oxyhemoglobin release by hemolysis, whereas higher concentrations produced a hemoglobin shift of valence, producing methemoglobin (>40 μg/mL). This substance is not degraded by proteases present in the venom. By infrared spectroscopy, starting in 80 μg/mL, we observed changes in bands that are associated with protein damage (1660 and 1540 cm−1) and lipid peroxidation (2960, 2920 and 1740 cm−1). Lipid peroxidation was confirmed by conjugated diene and thiobarbituric acid reactive substance determination, in which differences were observed between the control and erythrocytes treated with venom. Conclusions Crotalus molossus nigrescens venom provokes hemolysis and oxidative stress, which induces methemoglobin formation, loss of protein structure and lipid peroxidation.http://link.springer.com/article/10.1186/s40409-017-0114-yAttenuated total reflectance-Fourier transform infrared spectroscopyCrotalus molossus nigrescensVenomSnake venomMethemoglobinOxidative stress |
| spellingShingle | David Meléndez-Martínez Juan Manuel Muñoz Guillermo Barraza-Garza Martha Sandra Cruz-Peréz Ana Gatica-Colima Emilio Alvarez-Parrilla Luis Fernando Plenge-Tellechea Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes Journal of Venomous Animals and Toxins including Tropical Diseases Attenuated total reflectance-Fourier transform infrared spectroscopy Crotalus molossus nigrescens Venom Snake venom Methemoglobin Oxidative stress |
| title | Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| title_full | Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| title_fullStr | Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| title_full_unstemmed | Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| title_short | Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| title_sort | rattlesnake crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes |
| topic | Attenuated total reflectance-Fourier transform infrared spectroscopy Crotalus molossus nigrescens Venom Snake venom Methemoglobin Oxidative stress |
| url | http://link.springer.com/article/10.1186/s40409-017-0114-y |
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