Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants
Abstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100478&lng=en&tlng=en |
| _version_ | 1818313728498597888 |
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| author | Marcela Cristina Damião Andrucioli Mírian Aiko Nakane Matsumoto Sandra Yasuyo Fukada Maria Conceição Pereira Saraiva Ana Zilda Nazar Bergamo Fábio Lourenço Romano Raquel Assed Bezerra da Silva Lea Assed Bezerra da Silva Paulo Nelson-Filho |
| author_facet | Marcela Cristina Damião Andrucioli Mírian Aiko Nakane Matsumoto Sandra Yasuyo Fukada Maria Conceição Pereira Saraiva Ana Zilda Nazar Bergamo Fábio Lourenço Romano Raquel Assed Bezerra da Silva Lea Assed Bezerra da Silva Paulo Nelson-Filho |
| author_sort | Marcela Cristina Damião Andrucioli |
| collection | DOAJ |
| description | Abstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. Methodology: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). Results: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). Conclusions: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure. |
| first_indexed | 2024-12-13T08:38:22Z |
| format | Article |
| id | doaj.art-40b816d681094cc1b224258941484585 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-13T08:38:22Z |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-40b816d681094cc1b2242589414845852022-12-21T23:53:35ZengUniversity of São PauloJournal of Applied Oral Science1678-77652710.1590/1678-7757-2018-0476S1678-77572019000100478Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implantsMarcela Cristina Damião AndrucioliMírian Aiko Nakane MatsumotoSandra Yasuyo FukadaMaria Conceição Pereira SaraivaAna Zilda Nazar BergamoFábio Lourenço RomanoRaquel Assed Bezerra da SilvaLea Assed Bezerra da SilvaPaulo Nelson-FilhoAbstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. Methodology: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). Results: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). Conclusions: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100478&lng=en&tlng=enOrthodontic anchorage proceduresCytokinesRANKRANK ligandOsteoprotegerin |
| spellingShingle | Marcela Cristina Damião Andrucioli Mírian Aiko Nakane Matsumoto Sandra Yasuyo Fukada Maria Conceição Pereira Saraiva Ana Zilda Nazar Bergamo Fábio Lourenço Romano Raquel Assed Bezerra da Silva Lea Assed Bezerra da Silva Paulo Nelson-Filho Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants Journal of Applied Oral Science Orthodontic anchorage procedures Cytokines RANK RANK ligand Osteoprotegerin |
| title | Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants |
| title_full | Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants |
| title_fullStr | Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants |
| title_full_unstemmed | Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants |
| title_short | Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants |
| title_sort | quantification of pro inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini implants |
| topic | Orthodontic anchorage procedures Cytokines RANK RANK ligand Osteoprotegerin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100478&lng=en&tlng=en |
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