Single-cell sequencing technologies in bladder cancer research: Applications and challenges
Bladder cancer is among the most common malignant tumors with highly heterogeneous molecular characteristics. Despite advancements of the available therapeutic options, several bladder cancer patients exhibit unsatisfactory clinical outcomes. The lack of specific biomarkers for effective targeted th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1027909/full |
| _version_ | 1797990637919797248 |
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| author | Tianqi Lyu Yuanbin Lin Kerong Wu Zhanglei Cao Qian Zhang Jianping Zheng |
| author_facet | Tianqi Lyu Yuanbin Lin Kerong Wu Zhanglei Cao Qian Zhang Jianping Zheng |
| author_sort | Tianqi Lyu |
| collection | DOAJ |
| description | Bladder cancer is among the most common malignant tumors with highly heterogeneous molecular characteristics. Despite advancements of the available therapeutic options, several bladder cancer patients exhibit unsatisfactory clinical outcomes. The lack of specific biomarkers for effective targeted therapy or immunotherapy remains a major obstacle in treating bladder cancer. The rapid development of single-cell techniques is transforming our understanding of the intra-tumoral heterogeneity, thereby providing us with a powerful high-throughput sequencing tool that can reveal tumorigenesis, progression, and invasion in bladder tumors. In this review, we summarise and discuss how single-cell sequencing technologies have been applied in bladder cancer research, to advance our collective knowledge on the heterogeneity of bladder tumor cells, as well as to provide new insights into the complex ecosystem of the tumor microenvironment. The application of single-cell approaches also uncovers the therapeutic resistance mechanism in bladder cancer and facilitates the detection of urinary-exfoliated tumor cells. Moreover, benefiting from the powerful technical advantages of single-cell techniques, several key therapeutic targets and prognostic models of bladder cancer have been identified. It is hoped that this paper can provide novel insights into the precision medicine of bladder cancer. |
| first_indexed | 2024-04-11T08:39:41Z |
| format | Article |
| id | doaj.art-40bd624e21ae4a4f8bb632619fc992f0 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T08:39:41Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-40bd624e21ae4a4f8bb632619fc992f02022-12-22T04:34:14ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-10-011310.3389/fgene.2022.10279091027909Single-cell sequencing technologies in bladder cancer research: Applications and challengesTianqi Lyu0Yuanbin Lin1Kerong Wu2Zhanglei Cao3Qian Zhang4Jianping Zheng5Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science (CAS), Ningbo, ChinaCixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science (CAS), Ningbo, ChinaDepartment of Urology, Ningbo First Hospital, School of Medicine Ningbo University, Zhejiang University Ningbo Hospital, Ningbo, ChinaCixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science (CAS), Ningbo, ChinaCixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science (CAS), Ningbo, ChinaCixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science (CAS), Ningbo, ChinaBladder cancer is among the most common malignant tumors with highly heterogeneous molecular characteristics. Despite advancements of the available therapeutic options, several bladder cancer patients exhibit unsatisfactory clinical outcomes. The lack of specific biomarkers for effective targeted therapy or immunotherapy remains a major obstacle in treating bladder cancer. The rapid development of single-cell techniques is transforming our understanding of the intra-tumoral heterogeneity, thereby providing us with a powerful high-throughput sequencing tool that can reveal tumorigenesis, progression, and invasion in bladder tumors. In this review, we summarise and discuss how single-cell sequencing technologies have been applied in bladder cancer research, to advance our collective knowledge on the heterogeneity of bladder tumor cells, as well as to provide new insights into the complex ecosystem of the tumor microenvironment. The application of single-cell approaches also uncovers the therapeutic resistance mechanism in bladder cancer and facilitates the detection of urinary-exfoliated tumor cells. Moreover, benefiting from the powerful technical advantages of single-cell techniques, several key therapeutic targets and prognostic models of bladder cancer have been identified. It is hoped that this paper can provide novel insights into the precision medicine of bladder cancer.https://www.frontiersin.org/articles/10.3389/fgene.2022.1027909/fullsingle-cell sequencingbladder cancerheterogeneitybiomarkersprecision medicine |
| spellingShingle | Tianqi Lyu Yuanbin Lin Kerong Wu Zhanglei Cao Qian Zhang Jianping Zheng Single-cell sequencing technologies in bladder cancer research: Applications and challenges Frontiers in Genetics single-cell sequencing bladder cancer heterogeneity biomarkers precision medicine |
| title | Single-cell sequencing technologies in bladder cancer research: Applications and challenges |
| title_full | Single-cell sequencing technologies in bladder cancer research: Applications and challenges |
| title_fullStr | Single-cell sequencing technologies in bladder cancer research: Applications and challenges |
| title_full_unstemmed | Single-cell sequencing technologies in bladder cancer research: Applications and challenges |
| title_short | Single-cell sequencing technologies in bladder cancer research: Applications and challenges |
| title_sort | single cell sequencing technologies in bladder cancer research applications and challenges |
| topic | single-cell sequencing bladder cancer heterogeneity biomarkers precision medicine |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1027909/full |
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