Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis
<i>Rhizoctonia cerealis</i> is a worldwide soil-borne pathogenic fungus that significantly infects wheat and causes sharp eyespot in China. However, the biocontrol strains used for the control of <i>Rhizoctonia cerealis</i> are insufficient. In the present study, antagonistic...
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| author | Yanjie Yi Pengyu Luan Kang Wang Guiling Li Yanan Yin Yanhui Yang Qingyao Zhang Yang Liu |
| author_facet | Yanjie Yi Pengyu Luan Kang Wang Guiling Li Yanan Yin Yanhui Yang Qingyao Zhang Yang Liu |
| author_sort | Yanjie Yi |
| collection | DOAJ |
| description | <i>Rhizoctonia cerealis</i> is a worldwide soil-borne pathogenic fungus that significantly infects wheat and causes sharp eyespot in China. However, the biocontrol strains used for the control of <i>Rhizoctonia cerealis</i> are insufficient. In the present study, antagonistic strain B1302 from the rhizosphere of wheat were isolated and identified as <i>Bacillus mojovensis</i> based on their morphological, physiological, and biochemical characteristics, and their 16S rDNA sequence. Culture filtrate of strain B1302 had a broad antifungal spectrum. In order to improve the antifungal activity of B1302, response surface methodology (RSM) was used to optimize the culture conditions. The final medium composition and culture conditions were 13.2 g/L of wheat bran, 14.1 g/L of soybean meal, 224 r/min of rotation speed, 7.50 of initial pH, and 1.5 × 10<sup>8</sup> CFU/mL of inoculation amount at 35 °C for a culture duration of 72 h. <i>B. mojavensis</i> B1302 inhibited the hyphae growth of <i>R.</i><i>cerealis</i> and produced hydrolytic enzymes (protease, chitinase, and glucanase), IAA, and had N-fixing potentiality and P-solubilisation capacity. It can also promote wheat seedling growth in potted plants. The disease incidence and index of wheat seedlings were consistent with the effect of commercial pesticides under treatment with culture filtrate. The biocontrol efficacy of culture filtrate was significant—up to 65.25%. An animal toxicological safety analysis suggested that culture filtrate was safe for use and could be developed into an effective microbial fungicide to control wheat sharp eyespot. |
| first_indexed | 2024-03-09T04:03:26Z |
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| last_indexed | 2024-03-09T04:03:26Z |
| publishDate | 2022-08-01 |
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| series | Microorganisms |
| spelling | doaj.art-40c2499e3e1b4629ad0c4f4a5dcc15f22023-12-03T14:09:53ZengMDPI AGMicroorganisms2076-26072022-08-01108168210.3390/microorganisms10081682Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia CerealisYanjie Yi0Pengyu Luan1Kang Wang2Guiling Li3Yanan Yin4Yanhui Yang5Qingyao Zhang6Yang Liu7School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China<i>Rhizoctonia cerealis</i> is a worldwide soil-borne pathogenic fungus that significantly infects wheat and causes sharp eyespot in China. However, the biocontrol strains used for the control of <i>Rhizoctonia cerealis</i> are insufficient. In the present study, antagonistic strain B1302 from the rhizosphere of wheat were isolated and identified as <i>Bacillus mojovensis</i> based on their morphological, physiological, and biochemical characteristics, and their 16S rDNA sequence. Culture filtrate of strain B1302 had a broad antifungal spectrum. In order to improve the antifungal activity of B1302, response surface methodology (RSM) was used to optimize the culture conditions. The final medium composition and culture conditions were 13.2 g/L of wheat bran, 14.1 g/L of soybean meal, 224 r/min of rotation speed, 7.50 of initial pH, and 1.5 × 10<sup>8</sup> CFU/mL of inoculation amount at 35 °C for a culture duration of 72 h. <i>B. mojavensis</i> B1302 inhibited the hyphae growth of <i>R.</i><i>cerealis</i> and produced hydrolytic enzymes (protease, chitinase, and glucanase), IAA, and had N-fixing potentiality and P-solubilisation capacity. It can also promote wheat seedling growth in potted plants. The disease incidence and index of wheat seedlings were consistent with the effect of commercial pesticides under treatment with culture filtrate. The biocontrol efficacy of culture filtrate was significant—up to 65.25%. An animal toxicological safety analysis suggested that culture filtrate was safe for use and could be developed into an effective microbial fungicide to control wheat sharp eyespot.https://www.mdpi.com/2076-2607/10/8/1682<i>Bacillus mojovensis</i> B1302<i>Rhizoctonia cerealis</i>antifungal activityplant growth-promotionanimal toxicological safety |
| spellingShingle | Yanjie Yi Pengyu Luan Kang Wang Guiling Li Yanan Yin Yanhui Yang Qingyao Zhang Yang Liu Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis Microorganisms <i>Bacillus mojovensis</i> B1302 <i>Rhizoctonia cerealis</i> antifungal activity plant growth-promotion animal toxicological safety |
| title | Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis |
| title_full | Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis |
| title_fullStr | Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis |
| title_full_unstemmed | Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis |
| title_short | Antifungal Activity and Plant Growth-Promoting Properties of <i>Bacillus mojovensis</i> B1302 against Rhizoctonia Cerealis |
| title_sort | antifungal activity and plant growth promoting properties of i bacillus mojovensis i b1302 against rhizoctonia cerealis |
| topic | <i>Bacillus mojovensis</i> B1302 <i>Rhizoctonia cerealis</i> antifungal activity plant growth-promotion animal toxicological safety |
| url | https://www.mdpi.com/2076-2607/10/8/1682 |
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