Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs)
Objective To study the effects of curcumine on renal tubular epithelial-mesenchymal transition (EMT) and its possible mechanisms. Methods The effect of curcumin on the morphology of human kidney cells (HKCs) in the presence of TGF-β1 alone or in combination with different concentration curcumine was...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190629.pdf |
| _version_ | 1797365709281427456 |
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| author | CHEN Min-jia, DU Juan, YUAN Dan-feng, ZHANG Shu, HUANG Hong, ZHU Fang-qiang |
| author_facet | CHEN Min-jia, DU Juan, YUAN Dan-feng, ZHANG Shu, HUANG Hong, ZHU Fang-qiang |
| author_sort | CHEN Min-jia, DU Juan, YUAN Dan-feng, ZHANG Shu, HUANG Hong, ZHU Fang-qiang |
| collection | DOAJ |
| description | Objective To study the effects of curcumine on renal tubular epithelial-mesenchymal transition (EMT) and its possible mechanisms. Methods The effect of curcumin on the morphology of human kidney cells (HKCs) in the presence of TGF-β1 alone or in combination with different concentration curcumine was observed by phase contrast microscopy. The expressions of E-cardhrin, keratin, vimentin, alpha smooth muscle actin (α-SMA),fibroblast-specific protein 1 (FSP1) and key proteins of AKT/mTOR pathway were analyzed by immunohistochemical stain and Western blot. Results HKC cells showed a classic cobblestone morphology. Exposure of HKCs to TGF-β1 for 72 h induced a complete conversion of the epithelial cell to myofibroblast. When HKCs were co-incubated with TGF-β1 and various concentrations of curcumin for 72 h, curcumine maintained the epithelial morphology in a dose-dependent manner (from 12.5 to 50 μg/mL), antagonizing TGF-β1-induced the down-regulation expression of E-cardhrin, keratin and the up-rgulation of FSP1, vimentin and α-SMA. Importantly. Curcumine effectively suppressed the activity of the Akt/mTOR pathway in HKCs as demonstrated by a remarkable reduction of the Akt, mTOR, p70S6K, 4E-BP1和eIF4E phosphorylation. Conclusions Curcumine is a potent inhibitor of TGF-β1-induced epithelial-mesenchymal transition (EMT) and antagonizes TGF-β1-driven fibrogenesis through inhibition of the activity of the Akt/mTOR pathway. |
| first_indexed | 2024-03-08T16:53:46Z |
| format | Article |
| id | doaj.art-40c2586fca494b0db2104b8506666d8d |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:46Z |
| publishDate | 2020-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-40c2586fca494b0db2104b8506666d8d2024-01-05T03:13:53ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-01-014014853Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs)CHEN Min-jia, DU Juan, YUAN Dan-feng, ZHANG Shu, HUANG Hong, ZHU Fang-qiang01. State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042;;2. Department of Urology, the Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, ChinaObjective To study the effects of curcumine on renal tubular epithelial-mesenchymal transition (EMT) and its possible mechanisms. Methods The effect of curcumin on the morphology of human kidney cells (HKCs) in the presence of TGF-β1 alone or in combination with different concentration curcumine was observed by phase contrast microscopy. The expressions of E-cardhrin, keratin, vimentin, alpha smooth muscle actin (α-SMA),fibroblast-specific protein 1 (FSP1) and key proteins of AKT/mTOR pathway were analyzed by immunohistochemical stain and Western blot. Results HKC cells showed a classic cobblestone morphology. Exposure of HKCs to TGF-β1 for 72 h induced a complete conversion of the epithelial cell to myofibroblast. When HKCs were co-incubated with TGF-β1 and various concentrations of curcumin for 72 h, curcumine maintained the epithelial morphology in a dose-dependent manner (from 12.5 to 50 μg/mL), antagonizing TGF-β1-induced the down-regulation expression of E-cardhrin, keratin and the up-rgulation of FSP1, vimentin and α-SMA. Importantly. Curcumine effectively suppressed the activity of the Akt/mTOR pathway in HKCs as demonstrated by a remarkable reduction of the Akt, mTOR, p70S6K, 4E-BP1和eIF4E phosphorylation. Conclusions Curcumine is a potent inhibitor of TGF-β1-induced epithelial-mesenchymal transition (EMT) and antagonizes TGF-β1-driven fibrogenesis through inhibition of the activity of the Akt/mTOR pathway.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190629.pdfcurcumine|renal fibrosis|epithelialto-mesenchymal transition (emt)|tgf-β1|akt/mtor pathway |
| spellingShingle | CHEN Min-jia, DU Juan, YUAN Dan-feng, ZHANG Shu, HUANG Hong, ZHU Fang-qiang Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) Jichu yixue yu linchuang curcumine|renal fibrosis|epithelialto-mesenchymal transition (emt)|tgf-β1|akt/mtor pathway |
| title | Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) |
| title_full | Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) |
| title_fullStr | Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) |
| title_full_unstemmed | Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) |
| title_short | Curcumin inhibits TGF-β1-induced phenotypic transition of human kidney tubular cells (HKCs) |
| title_sort | curcumin inhibits tgf β1 induced phenotypic transition of human kidney tubular cells hkcs |
| topic | curcumine|renal fibrosis|epithelialto-mesenchymal transition (emt)|tgf-β1|akt/mtor pathway |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190629.pdf |
| work_keys_str_mv | AT chenminjiadujuanyuandanfengzhangshuhuanghongzhufangqiang curcumininhibitstgfb1inducedphenotypictransitionofhumankidneytubularcellshkcs |