Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis
Typically, biological systems are protected from the toxic effect of free radicals by antioxidant defense. Extracts from orchids have been reported to show high levels of exogenous antioxidant activity including Bulbophyllum orchids but so far, there have been no reports on antioxidant enzymes. Ther...
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| Format: | Article |
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Elsevier
2020-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580820300716 |
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| author | Pattana S. Huehne Kisana Bhinija Chantragan Srisomsap Daranee Chokchaichamnankit Churat Weeraphan Jisnuson Svasti Skorn Mongkolsuk |
| author_facet | Pattana S. Huehne Kisana Bhinija Chantragan Srisomsap Daranee Chokchaichamnankit Churat Weeraphan Jisnuson Svasti Skorn Mongkolsuk |
| author_sort | Pattana S. Huehne |
| collection | DOAJ |
| description | Typically, biological systems are protected from the toxic effect of free radicals by antioxidant defense. Extracts from orchids have been reported to show high levels of exogenous antioxidant activity including Bulbophyllum orchids but so far, there have been no reports on antioxidant enzymes. Therefore, differences in protein expression from leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl and Dendrobium Sonia Earsakul were studied using two-dimensional gel electrophoresis and mass spectrometry (LC/MS/MS). Interestingly, the largest group of these stress response proteins were associated with antioxidant defense and temperature stress, including superoxide dismutase (Cu–Zn) and heat shock protein 70. The high expression of this antioxidant enzyme from Bulbophyllum morphologlorum Kraenzl was confirmed by activity staining on native-PAGE, and the two Cu/Zn-SODs isoenzymes were identified as Cu/Zn-SOD 1 and Cu/Zn-SOD 2 by LC/MS/MS. The results suggested that Bulbophyllum orchid can be a potential plant source for medicines and natural antioxidant supplements. |
| first_indexed | 2024-12-13T12:34:24Z |
| format | Article |
| id | doaj.art-40c8e32e78ae4092a58f4637cb796f43 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-13T12:34:24Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-40c8e32e78ae4092a58f4637cb796f432022-12-21T23:45:55ZengElsevierBiochemistry and Biophysics Reports2405-58082020-07-0122Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysisPattana S. Huehne0Kisana Bhinija1Chantragan Srisomsap2Daranee Chokchaichamnankit3Churat Weeraphan4Jisnuson Svasti5Skorn Mongkolsuk6Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, 10210, ThailandLaboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, Thailand; Corresponding author.Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, Thailand; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok, 10210, ThailandLaboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, 10210, Thailand; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok, 10210, ThailandTypically, biological systems are protected from the toxic effect of free radicals by antioxidant defense. Extracts from orchids have been reported to show high levels of exogenous antioxidant activity including Bulbophyllum orchids but so far, there have been no reports on antioxidant enzymes. Therefore, differences in protein expression from leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl and Dendrobium Sonia Earsakul were studied using two-dimensional gel electrophoresis and mass spectrometry (LC/MS/MS). Interestingly, the largest group of these stress response proteins were associated with antioxidant defense and temperature stress, including superoxide dismutase (Cu–Zn) and heat shock protein 70. The high expression of this antioxidant enzyme from Bulbophyllum morphologlorum Kraenzl was confirmed by activity staining on native-PAGE, and the two Cu/Zn-SODs isoenzymes were identified as Cu/Zn-SOD 1 and Cu/Zn-SOD 2 by LC/MS/MS. The results suggested that Bulbophyllum orchid can be a potential plant source for medicines and natural antioxidant supplements.http://www.sciencedirect.com/science/article/pii/S2405580820300716BulbophyllumOrchidProteomicsStress responseEnzymatic antioxidantSuperoxide dismutase (Cu–Zn) |
| spellingShingle | Pattana S. Huehne Kisana Bhinija Chantragan Srisomsap Daranee Chokchaichamnankit Churat Weeraphan Jisnuson Svasti Skorn Mongkolsuk Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis Biochemistry and Biophysics Reports Bulbophyllum Orchid Proteomics Stress response Enzymatic antioxidant Superoxide dismutase (Cu–Zn) |
| title | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
| title_full | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
| title_fullStr | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
| title_full_unstemmed | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
| title_short | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
| title_sort | detection of superoxide dismutase cu zn isoenzymes in leaves and pseudobulbs of bulbophyllum morphologlorum kraenzl orchid by comparative proteomic analysis |
| topic | Bulbophyllum Orchid Proteomics Stress response Enzymatic antioxidant Superoxide dismutase (Cu–Zn) |
| url | http://www.sciencedirect.com/science/article/pii/S2405580820300716 |
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