Construction of a genetic map and QTL mapping of seed size traits in soybean
Soybean seed size and seed shape traits are closely related to plant yield and appearance quality. In this study, 186 individual plants of the F2 generation derived from crosses between Changjiang Chun 2 and JiYu 166 were selected as the mapping population to construct a molecular genetic linkage ma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1248315/full |
| _version_ | 1797737008479600640 |
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| author | Aohua Jiang Jiaqi Liu Weiran Gao Ronghan Ma Pingting Tan Fang Liu Jian Zhang |
| author_facet | Aohua Jiang Jiaqi Liu Weiran Gao Ronghan Ma Pingting Tan Fang Liu Jian Zhang |
| author_sort | Aohua Jiang |
| collection | DOAJ |
| description | Soybean seed size and seed shape traits are closely related to plant yield and appearance quality. In this study, 186 individual plants of the F2 generation derived from crosses between Changjiang Chun 2 and JiYu 166 were selected as the mapping population to construct a molecular genetic linkage map, and the phenotypic data of hundred-grain weight, seed length, seed width, and seed length-to-width ratio of soybean under three generations of F2 single plants and F2:3 and F2:4 lines were combined to detect the QTL (quantitative trait loci) for the corresponding traits by ICIM mapping. A soybean genetic map containing 455 markers with an average distance of 6.15 cM and a total length of 2799.2 cM was obtained. Forty-nine QTLs related to the hundred-grain weight, seed length, seed width, and seed length-to-width ratio of soybean were obtained under three environmental conditions. A total of 10 QTLs were detected in more than two environments with a phenotypic variation of over 10%. Twelve QTL clusters were identified on chromosomes 1, 2, 5, 6, 8, 13, 18, and 19, with the majority of the overlapping intervals for hundred-grain weight and seed width. These results will lay the theoretical and technical foundation for molecularly assisted breeding in soybean seed weight and seed shape. Eighteen candidate genes that may be involved in the regulation of soybean seed size were screened by gene functional annotation and GO enrichment analysis. |
| first_indexed | 2024-03-12T13:22:10Z |
| format | Article |
| id | doaj.art-40c8e47c932243f4ada0549f659f2fbf |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-12T13:22:10Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-40c8e47c932243f4ada0549f659f2fbf2023-08-25T18:00:33ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-08-011410.3389/fgene.2023.12483151248315Construction of a genetic map and QTL mapping of seed size traits in soybeanAohua JiangJiaqi LiuWeiran GaoRonghan MaPingting TanFang LiuJian ZhangSoybean seed size and seed shape traits are closely related to plant yield and appearance quality. In this study, 186 individual plants of the F2 generation derived from crosses between Changjiang Chun 2 and JiYu 166 were selected as the mapping population to construct a molecular genetic linkage map, and the phenotypic data of hundred-grain weight, seed length, seed width, and seed length-to-width ratio of soybean under three generations of F2 single plants and F2:3 and F2:4 lines were combined to detect the QTL (quantitative trait loci) for the corresponding traits by ICIM mapping. A soybean genetic map containing 455 markers with an average distance of 6.15 cM and a total length of 2799.2 cM was obtained. Forty-nine QTLs related to the hundred-grain weight, seed length, seed width, and seed length-to-width ratio of soybean were obtained under three environmental conditions. A total of 10 QTLs were detected in more than two environments with a phenotypic variation of over 10%. Twelve QTL clusters were identified on chromosomes 1, 2, 5, 6, 8, 13, 18, and 19, with the majority of the overlapping intervals for hundred-grain weight and seed width. These results will lay the theoretical and technical foundation for molecularly assisted breeding in soybean seed weight and seed shape. Eighteen candidate genes that may be involved in the regulation of soybean seed size were screened by gene functional annotation and GO enrichment analysis.https://www.frontiersin.org/articles/10.3389/fgene.2023.1248315/fullsoybeanseed sizegenetic linkage mappingQTLcandidate gene |
| spellingShingle | Aohua Jiang Jiaqi Liu Weiran Gao Ronghan Ma Pingting Tan Fang Liu Jian Zhang Construction of a genetic map and QTL mapping of seed size traits in soybean Frontiers in Genetics soybean seed size genetic linkage mapping QTL candidate gene |
| title | Construction of a genetic map and QTL mapping of seed size traits in soybean |
| title_full | Construction of a genetic map and QTL mapping of seed size traits in soybean |
| title_fullStr | Construction of a genetic map and QTL mapping of seed size traits in soybean |
| title_full_unstemmed | Construction of a genetic map and QTL mapping of seed size traits in soybean |
| title_short | Construction of a genetic map and QTL mapping of seed size traits in soybean |
| title_sort | construction of a genetic map and qtl mapping of seed size traits in soybean |
| topic | soybean seed size genetic linkage mapping QTL candidate gene |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1248315/full |
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