Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies
Abstract This study highlights the cytotoxic effect of three L. casei strains on colorectal cell lines in invitro conditions. Different concentrations of live, heat killed (HK) and cell free supernatant (CFS) of three L.casei strains were subjected to CaCo2 and MRC5 cell lines. The viability of the...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-09-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100701&tlng=en |
| _version_ | 1798035767377788928 |
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| author | Noroozi Elham Mojgani Naheed Motevaseli Elahe Modarressi Mohammad Hossein Tebianian Majid |
| author_facet | Noroozi Elham Mojgani Naheed Motevaseli Elahe Modarressi Mohammad Hossein Tebianian Majid |
| author_sort | Noroozi Elham |
| collection | DOAJ |
| description | Abstract This study highlights the cytotoxic effect of three L. casei strains on colorectal cell lines in invitro conditions. Different concentrations of live, heat killed (HK) and cell free supernatant (CFS) of three L.casei strains were subjected to CaCo2 and MRC5 cell lines. The viability of the treated and untreated cells was determined after 72 hrs by MTT assay, and IC50 estimated. Apoptosis was evaluated by Annexin V-propidium iodide method using flow cytometry. The live, HK and CFS of the L. casei strains showed cytotoxic effects on colorectal cell lines with significant differences. The cytotoxicity effects of live cells on CaCo2 cells were significantly higher (p˂0.01) than the HK cells. A dose dependent response was observed, as higher concentrations resulted in enhanced cytotoxicity effects. Live L.casei 1296-2cells inhibited 91% of CaCo2 cell growth, with IC50 of less than 108 cfu/ml. MRS medium and concentrations of CFS at above 20% v/v, were cytotoxic to the normal cell lines. Flow cytometry analyses of L. casei 1296-2 indicated that cytotoxicity effects on CaCo2 cells is related to apoptotic induction. Invitro studies indicate that Live and CFS of L. casei 1296-2 might be promising candidate for the control of colorectal cancers. |
| first_indexed | 2024-04-11T21:02:48Z |
| format | Article |
| id | doaj.art-40c9b1c5b4b946b09d35b4ff77a85de5 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-11T21:02:48Z |
| publishDate | 2022-09-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-40c9b1c5b4b946b09d35b4ff77a85de52022-12-22T04:03:27ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-09-015810.1590/s2175-97902022e19400Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studiesNoroozi ElhamMojgani Naheedhttps://orcid.org/0000-0002-3138-3433Motevaseli ElaheModarressi Mohammad HosseinTebianian MajidAbstract This study highlights the cytotoxic effect of three L. casei strains on colorectal cell lines in invitro conditions. Different concentrations of live, heat killed (HK) and cell free supernatant (CFS) of three L.casei strains were subjected to CaCo2 and MRC5 cell lines. The viability of the treated and untreated cells was determined after 72 hrs by MTT assay, and IC50 estimated. Apoptosis was evaluated by Annexin V-propidium iodide method using flow cytometry. The live, HK and CFS of the L. casei strains showed cytotoxic effects on colorectal cell lines with significant differences. The cytotoxicity effects of live cells on CaCo2 cells were significantly higher (p˂0.01) than the HK cells. A dose dependent response was observed, as higher concentrations resulted in enhanced cytotoxicity effects. Live L.casei 1296-2cells inhibited 91% of CaCo2 cell growth, with IC50 of less than 108 cfu/ml. MRS medium and concentrations of CFS at above 20% v/v, were cytotoxic to the normal cell lines. Flow cytometry analyses of L. casei 1296-2 indicated that cytotoxicity effects on CaCo2 cells is related to apoptotic induction. Invitro studies indicate that Live and CFS of L. casei 1296-2 might be promising candidate for the control of colorectal cancers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100701&tlng=enLactobacillus caseiProbioticParaprobioticPostbioticsColon cancerMTT AssayApoptosisFlowcytometry |
| spellingShingle | Noroozi Elham Mojgani Naheed Motevaseli Elahe Modarressi Mohammad Hossein Tebianian Majid Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies Brazilian Journal of Pharmaceutical Sciences Lactobacillus casei Probiotic Paraprobiotic Postbiotics Colon cancer MTT Assay Apoptosis Flowcytometry |
| title | Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies |
| title_full | Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies |
| title_fullStr | Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies |
| title_full_unstemmed | Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies |
| title_short | Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies |
| title_sort | selective cytotoxic effect of probiotic paraprobiotic and postbiotics of l casei strains against colorectal cancer cells invitro studies |
| topic | Lactobacillus casei Probiotic Paraprobiotic Postbiotics Colon cancer MTT Assay Apoptosis Flowcytometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100701&tlng=en |
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