Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L.
The current study aimed to assess the pharmacological potential of Justicia adhatoda by evaluating the presence of biologically active compounds using the gas chromatography–mass spectrometry approach and to undertake biological activities for the effectiveness of the present compounds using standar...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.922388/full |
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| author | Muhammad Musa Gul Jan Farzana Gul Jan Muhammad Hamayun Muhammad Irfan Muhammad Irfan Muhammad Irfan Abdur Rauf Abdulrahman Alsahammari Metab Alharbi Hafiz A. R. Suleria Niaz Ali |
| author_facet | Muhammad Musa Gul Jan Farzana Gul Jan Muhammad Hamayun Muhammad Irfan Muhammad Irfan Muhammad Irfan Abdur Rauf Abdulrahman Alsahammari Metab Alharbi Hafiz A. R. Suleria Niaz Ali |
| author_sort | Muhammad Musa |
| collection | DOAJ |
| description | The current study aimed to assess the pharmacological potential of Justicia adhatoda by evaluating the presence of biologically active compounds using the gas chromatography–mass spectrometry approach and to undertake biological activities for the effectiveness of the present compounds using standard tests. A total of 21 compounds were identified in the gas chromatography–mass spectrometry analysis of the ethyl acetate fraction in which 14 of the identified compounds are recognized for their pharmacological potential in the literature. In total, four fractions (ethyl acetate, chloroform, n-hexane, and aqueous) were evaluated for pharmacological activities. In carrageenan-induced inflammation, the chloroform fraction exhibited high anti-inflammatory activity (46.51%). Similarly, the analgesic potential of ethyl acetate fraction was the most effective (300 mg/kg) in the acetic acid-induced test. Similarly, in the formalin test, ethyl acetate fraction exhibited maximum inhibition in both early (74.35%) and late phases (88.38). Maximum inhibition of pyrexia (77.98%) was recorded for the ethyl acetate fraction (300 mg/kg). In DPPH assay, the ethyl acetate fraction revealed the highest scavenging potential among other fractions (50 μg/ml resulted in 50.40% and 100 μg/ml resulted in 66.74% scavenging). |
| first_indexed | 2024-12-10T12:35:06Z |
| format | Article |
| id | doaj.art-40ca1b27a02543aca88181df4c097992 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T12:35:06Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-40ca1b27a02543aca88181df4c0979922022-12-22T01:48:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-09-011310.3389/fphar.2022.922388922388Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L.Muhammad Musa0Gul Jan1Farzana Gul Jan2Muhammad Hamayun3Muhammad Irfan4Muhammad Irfan5Muhammad Irfan6Abdur Rauf7Abdulrahman Alsahammari8Metab Alharbi9Hafiz A. R. Suleria10Niaz Ali11Department of Botany, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, University of Swabi, Swabi, PakistanMissouri Botanical Garden, St. Louis, MO, United StatesDepartment of Chemistry, University of Swabi, Swabi, PakistanDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaFaculty of Veterinary and Agricultural Sciences, School of Agriculture and Food, The University of Melbourne, Parkville, VIC, AustraliaDepartment of Botany, Hazara University, Mansehra, PakistanThe current study aimed to assess the pharmacological potential of Justicia adhatoda by evaluating the presence of biologically active compounds using the gas chromatography–mass spectrometry approach and to undertake biological activities for the effectiveness of the present compounds using standard tests. A total of 21 compounds were identified in the gas chromatography–mass spectrometry analysis of the ethyl acetate fraction in which 14 of the identified compounds are recognized for their pharmacological potential in the literature. In total, four fractions (ethyl acetate, chloroform, n-hexane, and aqueous) were evaluated for pharmacological activities. In carrageenan-induced inflammation, the chloroform fraction exhibited high anti-inflammatory activity (46.51%). Similarly, the analgesic potential of ethyl acetate fraction was the most effective (300 mg/kg) in the acetic acid-induced test. Similarly, in the formalin test, ethyl acetate fraction exhibited maximum inhibition in both early (74.35%) and late phases (88.38). Maximum inhibition of pyrexia (77.98%) was recorded for the ethyl acetate fraction (300 mg/kg). In DPPH assay, the ethyl acetate fraction revealed the highest scavenging potential among other fractions (50 μg/ml resulted in 50.40% and 100 μg/ml resulted in 66.74% scavenging).https://www.frontiersin.org/articles/10.3389/fphar.2022.922388/fullantioxidantanalgesicantispasmodicanti-inflammatoryantibacterialmedicinal plants |
| spellingShingle | Muhammad Musa Gul Jan Farzana Gul Jan Muhammad Hamayun Muhammad Irfan Muhammad Irfan Muhammad Irfan Abdur Rauf Abdulrahman Alsahammari Metab Alharbi Hafiz A. R. Suleria Niaz Ali Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. Frontiers in Pharmacology antioxidant analgesic antispasmodic anti-inflammatory antibacterial medicinal plants |
| title | Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. |
| title_full | Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. |
| title_fullStr | Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. |
| title_full_unstemmed | Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. |
| title_short | Pharmacological activities and gas chromatography–mass spectrometry analysis for the identification of bioactive compounds from Justicia adhatoda L. |
| title_sort | pharmacological activities and gas chromatography mass spectrometry analysis for the identification of bioactive compounds from justicia adhatoda l |
| topic | antioxidant analgesic antispasmodic anti-inflammatory antibacterial medicinal plants |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.922388/full |
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