Meiotic spindle size is a strong indicator of human oocyte quality
Abstract Purpose To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI). Methods Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years un...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-07-01
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| Series: | Reproductive Medicine and Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/rmb2.12100 |
| _version_ | 1818158848903479296 |
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| author | Hiroyuki Tomari Ko Honjo Katsuko Kunitake Natsumi Aramaki Saori Kuhara Naomi Hidaka Kayoko Nishimura Yumi Nagata Toshitaka Horiuchi |
| author_facet | Hiroyuki Tomari Ko Honjo Katsuko Kunitake Natsumi Aramaki Saori Kuhara Naomi Hidaka Kayoko Nishimura Yumi Nagata Toshitaka Horiuchi |
| author_sort | Hiroyuki Tomari |
| collection | DOAJ |
| description | Abstract Purpose To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI). Methods Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI. The oocytes were classified into three groups, according to spindle size: group A (<90 μm2), group B (90‐120 μm2), and group C (>120 μm2). Results Overall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups. Conclusion The oocytes with a spindle size of 90‐120 μm2 showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically. |
| first_indexed | 2024-12-11T15:36:37Z |
| format | Article |
| id | doaj.art-40cb9d4909da42b385d11bc5af074fa4 |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-12-11T15:36:37Z |
| publishDate | 2018-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-40cb9d4909da42b385d11bc5af074fa42022-12-22T00:59:55ZengWileyReproductive Medicine and Biology1445-57811447-05782018-07-0117326827410.1002/rmb2.12100Meiotic spindle size is a strong indicator of human oocyte qualityHiroyuki Tomari0Ko Honjo1Katsuko Kunitake2Natsumi Aramaki3Saori Kuhara4Naomi Hidaka5Kayoko Nishimura6Yumi Nagata7Toshitaka Horiuchi8IVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanIVF Nagata Clinic Fukuoka JapanGraduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Hiroshima JapanAbstract Purpose To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI). Methods Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI. The oocytes were classified into three groups, according to spindle size: group A (<90 μm2), group B (90‐120 μm2), and group C (>120 μm2). Results Overall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups. Conclusion The oocytes with a spindle size of 90‐120 μm2 showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically.https://doi.org/10.1002/rmb2.12100assisted reproductive technologyblastocystfertilizationintracytoplasmic sperm injectionmeiotic spindle |
| spellingShingle | Hiroyuki Tomari Ko Honjo Katsuko Kunitake Natsumi Aramaki Saori Kuhara Naomi Hidaka Kayoko Nishimura Yumi Nagata Toshitaka Horiuchi Meiotic spindle size is a strong indicator of human oocyte quality Reproductive Medicine and Biology assisted reproductive technology blastocyst fertilization intracytoplasmic sperm injection meiotic spindle |
| title | Meiotic spindle size is a strong indicator of human oocyte quality |
| title_full | Meiotic spindle size is a strong indicator of human oocyte quality |
| title_fullStr | Meiotic spindle size is a strong indicator of human oocyte quality |
| title_full_unstemmed | Meiotic spindle size is a strong indicator of human oocyte quality |
| title_short | Meiotic spindle size is a strong indicator of human oocyte quality |
| title_sort | meiotic spindle size is a strong indicator of human oocyte quality |
| topic | assisted reproductive technology blastocyst fertilization intracytoplasmic sperm injection meiotic spindle |
| url | https://doi.org/10.1002/rmb2.12100 |
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