Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts
The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1333865/full |
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| author | Sakina Yagi Sakina Yagi Nilofar Nilofar Nilofar Nilofar Abdullahi Ibrahim Uba Giovanni Caprioli Ahmed M. Mustafa Simone Angeloni Ismail Koyuncu Fatma Seker Rıdvan Polat Sumaiya Jahan Supti Faria Tasnim Yusra Al Dhaheri Gokhan Zengin Ali H. Eid |
| author_facet | Sakina Yagi Sakina Yagi Nilofar Nilofar Nilofar Nilofar Abdullahi Ibrahim Uba Giovanni Caprioli Ahmed M. Mustafa Simone Angeloni Ismail Koyuncu Fatma Seker Rıdvan Polat Sumaiya Jahan Supti Faria Tasnim Yusra Al Dhaheri Gokhan Zengin Ali H. Eid |
| author_sort | Sakina Yagi |
| collection | DOAJ |
| description | The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 μg g−1) and p-coumaric acid (3044.01 μg g−1) while the highest accumulation of kaempferol-3-glucoside (1537.38 μg g−1), caffeic acid (130.13 μg g−1) and 4-hydroxy benzoic acid (465.93 μg g−1) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03–73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC50 of 8.71 μg/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of V. diversifolium aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer’s disease among others. |
| first_indexed | 2024-03-08T09:36:42Z |
| format | Article |
| id | doaj.art-40cbb516212c4fe08dc4a0847a62acb3 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-08T09:36:42Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-40cbb516212c4fe08dc4a0847a62acb32024-01-30T08:07:08ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-01-011510.3389/fphar.2024.13338651333865Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extractsSakina Yagi0Sakina Yagi1Nilofar Nilofar2Nilofar Nilofar3Abdullahi Ibrahim Uba4Giovanni Caprioli5Ahmed M. Mustafa6Simone Angeloni7Ismail Koyuncu8Fatma Seker9Rıdvan Polat10Sumaiya Jahan Supti11Faria Tasnim12Yusra Al Dhaheri13Gokhan Zengin14Ali H. Eid15Department of Botany, Faculty of Science, University of Khartoum, Khartoum, SudanUniversité de Lorraine, INRAE, LAE, Nancy, FrancePhysiology and Biochemistry Research Laborotory, Department of Biology, Science Faculty, Selcuk University, Konya, TürkiyeDepartment of Pharmacy, Botanic Garden “Giardino dei Semplici” “Gabriele d’Annunzio” University, Chieti, ItalyDepartment of Molecular Biology and Genetics, Istanbul AREL University, Istanbul, TürkiyeSchool of Pharmacy, University of Camerino, Camerino, ItalySchool of Pharmacy, University of Camerino, Camerino, ItalySchool of Pharmacy, University of Camerino, Camerino, ItalyDepartment of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, TürkiyeDepartment of Biology, Science Arts Faculty, Harran University, Sanliurfa, TürkiyeDepartment of Landscape Architecture, Faculty of Agriculture, Bingol University, Bingöl, Türkiye0Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh0Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh1Department of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesPhysiology and Biochemistry Research Laborotory, Department of Biology, Science Faculty, Selcuk University, Konya, Türkiye2Department of Basic Medical Sciences, College of Medicine, QU Health, Doha, QatarThe present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 μg g−1) and p-coumaric acid (3044.01 μg g−1) while the highest accumulation of kaempferol-3-glucoside (1537.38 μg g−1), caffeic acid (130.13 μg g−1) and 4-hydroxy benzoic acid (465.93 μg g−1) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03–73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC50 of 8.71 μg/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of V. diversifolium aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer’s disease among others.https://www.frontiersin.org/articles/10.3389/fphar.2024.1333865/fullcancercardiovascular diseaseHerbal medicineoxidative stressreactive oxgen species (ROS) |
| spellingShingle | Sakina Yagi Sakina Yagi Nilofar Nilofar Nilofar Nilofar Abdullahi Ibrahim Uba Giovanni Caprioli Ahmed M. Mustafa Simone Angeloni Ismail Koyuncu Fatma Seker Rıdvan Polat Sumaiya Jahan Supti Faria Tasnim Yusra Al Dhaheri Gokhan Zengin Ali H. Eid Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts Frontiers in Pharmacology cancer cardiovascular disease Herbal medicine oxidative stress reactive oxgen species (ROS) |
| title | Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts |
| title_full | Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts |
| title_fullStr | Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts |
| title_full_unstemmed | Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts |
| title_short | Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts |
| title_sort | elucidating the chemical profile and biological studies of verbascum diversifolium hochst extracts |
| topic | cancer cardiovascular disease Herbal medicine oxidative stress reactive oxgen species (ROS) |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1333865/full |
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