Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study
In many occupational settings, workers are frequently exposed to toluene and noise. However, the individual and combined effects of these exposures on the cardiovascular system have not been fully elucidated. Therefore, this study aimed to investigate the impact of simultaneous exposure to toluene a...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016423001743 |
| _version_ | 1797754016957988864 |
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| author | Takoua Ben Attia Ridha Ben Ali Afef Nahdi Said Galai Ridha Ghali Soumeya Rammeh Michèle Véronique El may Abada Mhamdi |
| author_facet | Takoua Ben Attia Ridha Ben Ali Afef Nahdi Said Galai Ridha Ghali Soumeya Rammeh Michèle Véronique El may Abada Mhamdi |
| author_sort | Takoua Ben Attia |
| collection | DOAJ |
| description | In many occupational settings, workers are frequently exposed to toluene and noise. However, the individual and combined effects of these exposures on the cardiovascular system have not been fully elucidated. Therefore, this study aimed to investigate the impact of simultaneous exposure to toluene and noise on the rat heart, while also evaluating the potential preventive effect of olive leaf extract (OLE). Forty-eight male Wistar rats were randomly assigned to eight groups (n = 6/group): control group (C), control group that received OLE (C + OLE), group exposed to noise (N), group exposed to noise and receiving OLE (N + OLE), group exposed to toluene (T), group exposed to toluene and receiving OLE (T + OLE), group co-exposed to noise and toluene (NT), and group co-exposed to noise and toluene and receiving OLE (NT + OLE). The rats in this study were subjected to simultaneous exposure to toluene and noise for a duration of six weeks, within a custom-built plexiglass chamber. Toluene was administered at a concentration of 300 ppm, while the noise level was set to 85 dB(A). The exposure chamber was equipped with a generation system, an exposure system, and a monitoring system, ensuring precise and accurate exposure conditions. After the six-week period, heart and blood samples were collected from the rats for subsequent analysis. Plasma levels of cholesterol (CHOL), triglycerides (TG), lactate dehydrogenase (LDH), and creatine kinase (CK) were measured, and histopathological investigation was conducted using HE staining. Additionally, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels in heart tissue were measured. Our results showed that simultaneous exposure to noise and toluene altered CHOL, TG, LDH, and CK levels, and also caused an increase in lipid peroxidation levels and superoxide dismutase activity, along with a decrease in catalase activity in the heart. A significant alteration in the myocardium was also observed. However, treatment with OLE was found to modulate these oxidative and histological changes, ultimately correcting the deleterious effects induced by the combined exposure to noise and toluene. Therefore, our study suggests that OLE could be a potential preventive measure for individuals exposed to toluene and noise in industrial settings. |
| first_indexed | 2024-03-12T17:27:20Z |
| format | Article |
| id | doaj.art-40d04cc5c61d4d04869a09298fb5921c |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-03-12T17:27:20Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-40d04cc5c61d4d04869a09298fb5921c2023-08-05T05:15:16ZengElsevierSaudi Pharmaceutical Journal1319-01642023-08-01318101683Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental studyTakoua Ben Attia0Ridha Ben Ali1Afef Nahdi2Said Galai3Ridha Ghali4Soumeya Rammeh5Michèle Véronique El may6Abada Mhamdi7University of Tunis El Manar, Research Unit n° 17/ES/13, Inflammation, Cell Proliferation and Cell Death, Faculty of Medicine of Tunis, Tunis, Tunisia; Corresponding author at: Université Tunis el Manar Tunisie.University of Tunis El Manar, Research Unit n° 17/ES/13, Inflammation, Cell Proliferation and Cell Death, Faculty of Medicine of Tunis, Tunis, Tunisia; University of Tunis El Manar, Tunis, Unit of Experimental Medicine, Faculty of Medicine of Tunis, TunisiaUniversity of Tunis El Manar, Research Unit n° 17/ES/13, Inflammation, Cell Proliferation and Cell Death, Faculty of Medicine of Tunis, Tunis, TunisiaResearch Laboratory of Neurological Diseases of the Child (LR18SP04)-Department of Clinical Biology -National Institute Mongi Ben Hmida of Neurology at Tunis, TunisiaLaboratory of Environmental Toxicology Research, Faculty of Pharmaceutical Sciences, University of Monastir, Monastir, TunisiaUniversity of Tunis El Manar, Tunis, Research Unit n° 17ES15- Department of Pathology, Charles Nicolle Hospital, Tunis, TunisiaUniversity of Tunis El Manar, Research Unit n° 17/ES/13, Inflammation, Cell Proliferation and Cell Death, Faculty of Medicine of Tunis, Tunis, Tunisia; University of Tunis El Manar, Tunis, Unit of Experimental Medicine, Faculty of Medicine of Tunis, TunisiaUniversity of Tunis El Manar, Research Unit n° 17/ES/13, Inflammation, Cell Proliferation and Cell Death, Faculty of Medicine of Tunis, Tunis, TunisiaIn many occupational settings, workers are frequently exposed to toluene and noise. However, the individual and combined effects of these exposures on the cardiovascular system have not been fully elucidated. Therefore, this study aimed to investigate the impact of simultaneous exposure to toluene and noise on the rat heart, while also evaluating the potential preventive effect of olive leaf extract (OLE). Forty-eight male Wistar rats were randomly assigned to eight groups (n = 6/group): control group (C), control group that received OLE (C + OLE), group exposed to noise (N), group exposed to noise and receiving OLE (N + OLE), group exposed to toluene (T), group exposed to toluene and receiving OLE (T + OLE), group co-exposed to noise and toluene (NT), and group co-exposed to noise and toluene and receiving OLE (NT + OLE). The rats in this study were subjected to simultaneous exposure to toluene and noise for a duration of six weeks, within a custom-built plexiglass chamber. Toluene was administered at a concentration of 300 ppm, while the noise level was set to 85 dB(A). The exposure chamber was equipped with a generation system, an exposure system, and a monitoring system, ensuring precise and accurate exposure conditions. After the six-week period, heart and blood samples were collected from the rats for subsequent analysis. Plasma levels of cholesterol (CHOL), triglycerides (TG), lactate dehydrogenase (LDH), and creatine kinase (CK) were measured, and histopathological investigation was conducted using HE staining. Additionally, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels in heart tissue were measured. Our results showed that simultaneous exposure to noise and toluene altered CHOL, TG, LDH, and CK levels, and also caused an increase in lipid peroxidation levels and superoxide dismutase activity, along with a decrease in catalase activity in the heart. A significant alteration in the myocardium was also observed. However, treatment with OLE was found to modulate these oxidative and histological changes, ultimately correcting the deleterious effects induced by the combined exposure to noise and toluene. Therefore, our study suggests that OLE could be a potential preventive measure for individuals exposed to toluene and noise in industrial settings.http://www.sciencedirect.com/science/article/pii/S1319016423001743NoiseTolueneHeart tissueOlea Europaea L. leaf extractRatAntioxidant activity |
| spellingShingle | Takoua Ben Attia Ridha Ben Ali Afef Nahdi Said Galai Ridha Ghali Soumeya Rammeh Michèle Véronique El may Abada Mhamdi Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study Saudi Pharmaceutical Journal Noise Toluene Heart tissue Olea Europaea L. leaf extract Rat Antioxidant activity |
| title | Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study |
| title_full | Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study |
| title_fullStr | Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study |
| title_full_unstemmed | Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study |
| title_short | Olea europaea L. leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene: An experimental study |
| title_sort | olea europaea l leaf extract mitigates oxidative and histological damage in rat heart tissue exposed to combined noise and toluene an experimental study |
| topic | Noise Toluene Heart tissue Olea Europaea L. leaf extract Rat Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S1319016423001743 |
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