Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84835-0 |
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| author | Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon |
| author_facet | Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon |
| author_sort | Mohammad Ebrahim Asadi Zarch |
| collection | DOAJ |
| description | Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function. |
| first_indexed | 2024-12-23T04:00:29Z |
| format | Article |
| id | doaj.art-40d77a9f1bd641a09d77081f546e4c67 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T04:00:29Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-40d77a9f1bd641a09d77081f546e4c672022-12-21T18:00:44ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-84835-0Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approachMohammad Ebrahim Asadi Zarch0Alireza Afshar1Farhad Rahmanifar2Mohammad Reza Jafarzadeh Shirazi3Mandana Baghban4Mohammad Dadpasand5Farzad Mohammad Rezazadeh6Arezoo Khoradmehr7Hossein Baharvand8Amin Tamadon9Department of Animal Sciences, College of Agriculture, Shiraz UniversityThe Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical SciencesDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz UniversityDepartment of Animal Sciences, College of Agriculture, Shiraz UniversityDepartment of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical SciencesDepartment of Animal Sciences, College of Agriculture, Shiraz UniversityDepartment of Animal Sciences, College of Agriculture, Shiraz UniversityThe Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical SciencesDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECRThe Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical SciencesAbstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function.https://doi.org/10.1038/s41598-021-84835-0 |
| spellingShingle | Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach Scientific Reports |
| title | Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach |
| title_full | Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach |
| title_fullStr | Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach |
| title_full_unstemmed | Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach |
| title_short | Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach |
| title_sort | three dimensional and two dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary a golgi cox staining approach |
| url | https://doi.org/10.1038/s41598-021-84835-0 |
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