Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae)
Alpinia purpurata is an ornamental plant species native to the Asia-Pacific with commercial potential due to its red inflorescence adapted to the northern Colombian coast; however, the lack of propagation protocols hinders commercial cultivation. To adjust a micropropagation protocol for clonal pla...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Corporación Colombiana de Investigación Agropecuaria (Corpoica)
2020-05-01
|
| Series: | Ciencia y Tecnología Agropecuaria |
| Subjects: | |
| Online Access: | https://revistacta.agrosavia.co/index.php/revista/article/view/1193 |
| _version_ | 1797331406892826624 |
|---|---|
| author | Isidro Elías Suarez Padrón Pablo Miguel Pérez Meza Claudia Marcela Lopez Diaz |
| author_facet | Isidro Elías Suarez Padrón Pablo Miguel Pérez Meza Claudia Marcela Lopez Diaz |
| author_sort | Isidro Elías Suarez Padrón |
| collection | DOAJ |
| description |
Alpinia purpurata is an ornamental plant species native to the Asia-Pacific with commercial potential due to its red inflorescence adapted to the northern Colombian coast; however, the lack of propagation protocols hinders commercial cultivation. To adjust a micropropagation protocol for clonal plant material production, the aim of this study was to evaluate the effect of sucrose and gibberellic acid (GA3) in the culture medium on the number of new shoots per explant, shoot length, root number, root length and number of leaves. Explants were cultured in semisolid MS (Murashige & Skoog) medium with sucrose (30, 45 and 60 mg/L) and GA3 (0.0; 0.25; 0.5; 0.75 and 1.0 mg/L). A factorial experimental design with two factors was employed, with 15 treatments, 10 replicates per treatment and 150 experimental units distributed with a completely randomized design. Cultures were stored five weeks at 25 °C with a 12 h photoperiod (40 μmol m-2 s-1). Anova and Tukey’s test allowed detecting the statistical effects of sucrose and GA3 supply for all variables. An increase in sucrose concentration resulted in a higher number of shoots, while an increase in GA3 induced longer shoots. Root length and leaf number decreased as GA3 and sucrose increased.
|
| first_indexed | 2024-03-08T07:33:59Z |
| format | Article |
| id | doaj.art-40d81fad1bce42c29468d7e11b817d19 |
| institution | Directory Open Access Journal |
| issn | 2500-5308 |
| language | English |
| last_indexed | 2024-03-08T07:33:59Z |
| publishDate | 2020-05-01 |
| publisher | Corporación Colombiana de Investigación Agropecuaria (Corpoica) |
| record_format | Article |
| series | Ciencia y Tecnología Agropecuaria |
| spelling | doaj.art-40d81fad1bce42c29468d7e11b817d192024-02-02T19:19:38ZengCorporación Colombiana de Investigación Agropecuaria (Corpoica)Ciencia y Tecnología Agropecuaria2500-53082020-05-0121210.21930/rcta.vol21_num2_art:1193Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae)Isidro Elías Suarez Padrón0Pablo Miguel Pérez Meza1Claudia Marcela Lopez Diaz2Universidad de CórdobaUniversidad de CórdobaUniversidad de Córdoba Alpinia purpurata is an ornamental plant species native to the Asia-Pacific with commercial potential due to its red inflorescence adapted to the northern Colombian coast; however, the lack of propagation protocols hinders commercial cultivation. To adjust a micropropagation protocol for clonal plant material production, the aim of this study was to evaluate the effect of sucrose and gibberellic acid (GA3) in the culture medium on the number of new shoots per explant, shoot length, root number, root length and number of leaves. Explants were cultured in semisolid MS (Murashige & Skoog) medium with sucrose (30, 45 and 60 mg/L) and GA3 (0.0; 0.25; 0.5; 0.75 and 1.0 mg/L). A factorial experimental design with two factors was employed, with 15 treatments, 10 replicates per treatment and 150 experimental units distributed with a completely randomized design. Cultures were stored five weeks at 25 °C with a 12 h photoperiod (40 μmol m-2 s-1). Anova and Tukey’s test allowed detecting the statistical effects of sucrose and GA3 supply for all variables. An increase in sucrose concentration resulted in a higher number of shoots, while an increase in GA3 induced longer shoots. Root length and leaf number decreased as GA3 and sucrose increased. https://revistacta.agrosavia.co/index.php/revista/article/view/1193Inflorescencemicropropagationornamental plantsplant growth substancesred ginger |
| spellingShingle | Isidro Elías Suarez Padrón Pablo Miguel Pérez Meza Claudia Marcela Lopez Diaz Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) Ciencia y Tecnología Agropecuaria Inflorescence micropropagation ornamental plants plant growth substances red ginger |
| title | Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) |
| title_full | Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) |
| title_fullStr | Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) |
| title_full_unstemmed | Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) |
| title_short | Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae) |
| title_sort | evaluation of sucrose and ga3 in an in vitro shoot culture of alpinia purpurata zingiberaceae |
| topic | Inflorescence micropropagation ornamental plants plant growth substances red ginger |
| url | https://revistacta.agrosavia.co/index.php/revista/article/view/1193 |
| work_keys_str_mv | AT isidroeliassuarezpadron evaluationofsucroseandga3inaninvitroshootcultureofalpiniapurpuratazingiberaceae AT pablomiguelperezmeza evaluationofsucroseandga3inaninvitroshootcultureofalpiniapurpuratazingiberaceae AT claudiamarcelalopezdiaz evaluationofsucroseandga3inaninvitroshootcultureofalpiniapurpuratazingiberaceae |