Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation
Microglial activation elicits an immune response by producing proinflammatory modulators and cytokines that cause neurodegeneration. Therefore, a plausible strategy to prevent neurodegeneration is to inhibit neuroinflammation caused by microglial activation. Myricetin, a natural flavanol, induces ne...
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MDPI AG
2020-01-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/10/1/32 |
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| author | Jung-Hee Jang Seung Hoon Lee Kyungsook Jung Horyong Yoo Gunhyuk Park |
| author_facet | Jung-Hee Jang Seung Hoon Lee Kyungsook Jung Horyong Yoo Gunhyuk Park |
| author_sort | Jung-Hee Jang |
| collection | DOAJ |
| description | Microglial activation elicits an immune response by producing proinflammatory modulators and cytokines that cause neurodegeneration. Therefore, a plausible strategy to prevent neurodegeneration is to inhibit neuroinflammation caused by microglial activation. Myricetin, a natural flavanol, induces neuroprotective effects by inhibiting inflammation and oxidative stress. However, whether myricetin inhibits lipopolysaccharide (LPS)-induced neuroinflammation in hippocampus and cortex regions is not known. To test this, we examined the effects of myricetin on LPS-induced neuroinflammation in a microglial BV2 cell line. We found that myricetin significantly downregulated several markers of the neuroinflammatory response in LPS-induced activated microglia, including inducible nitric oxide (NO) synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory modulators and cytokines such as prostaglandin E2 (PGE<sub>2</sub>), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α). Moreover, myricetin suppressed the expression of c-Jun NH2-terminal kinase (JNK), p38 MAPK, and extracellular signal-regulated kinase (ERK), which are components of the mitogen-activated protein kinase (MAPK) signaling pathway. Furthermore, myricetin inhibited LPS-induced macrophages and microglial activation in the hippocampus and cortex of mice. Based on our results, we suggest that myricetin inhibits neuroinflammation in BV2 microglia by inhibiting the MAPK signaling pathway and the production of proinflammatory modulators and cytokines. Therefore, this could potentially be used for the treatment of neuroinflammatory diseases. |
| first_indexed | 2024-12-21T21:50:58Z |
| format | Article |
| id | doaj.art-40d86bffd3b24d69b328dd06ead898d4 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-12-21T21:50:58Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-40d86bffd3b24d69b328dd06ead898d42022-12-21T18:49:05ZengMDPI AGBrain Sciences2076-34252020-01-011013210.3390/brainsci10010032brainsci10010032Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced NeuroinflammationJung-Hee Jang0Seung Hoon Lee1Kyungsook Jung2Horyong Yoo3Gunhyuk Park4Department of Neurologic Disorders & Aging Brain Constitution, Dunsan Korean Medicine Hospital, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaNatural Product Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 56212, KoreaDepartment of Neurologic Disorders & Aging Brain Constitution, Dunsan Korean Medicine Hospital, Daejeon 34054, KoreaHerbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Jeollanam-do 58245, KoreaMicroglial activation elicits an immune response by producing proinflammatory modulators and cytokines that cause neurodegeneration. Therefore, a plausible strategy to prevent neurodegeneration is to inhibit neuroinflammation caused by microglial activation. Myricetin, a natural flavanol, induces neuroprotective effects by inhibiting inflammation and oxidative stress. However, whether myricetin inhibits lipopolysaccharide (LPS)-induced neuroinflammation in hippocampus and cortex regions is not known. To test this, we examined the effects of myricetin on LPS-induced neuroinflammation in a microglial BV2 cell line. We found that myricetin significantly downregulated several markers of the neuroinflammatory response in LPS-induced activated microglia, including inducible nitric oxide (NO) synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory modulators and cytokines such as prostaglandin E2 (PGE<sub>2</sub>), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α). Moreover, myricetin suppressed the expression of c-Jun NH2-terminal kinase (JNK), p38 MAPK, and extracellular signal-regulated kinase (ERK), which are components of the mitogen-activated protein kinase (MAPK) signaling pathway. Furthermore, myricetin inhibited LPS-induced macrophages and microglial activation in the hippocampus and cortex of mice. Based on our results, we suggest that myricetin inhibits neuroinflammation in BV2 microglia by inhibiting the MAPK signaling pathway and the production of proinflammatory modulators and cytokines. Therefore, this could potentially be used for the treatment of neuroinflammatory diseases.https://www.mdpi.com/2076-3425/10/1/32myricetininflammationmicroglialipopolysaccharide-induced neuroinflammationcytokines |
| spellingShingle | Jung-Hee Jang Seung Hoon Lee Kyungsook Jung Horyong Yoo Gunhyuk Park Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation Brain Sciences myricetin inflammation microglia lipopolysaccharide-induced neuroinflammation cytokines |
| title | Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation |
| title_full | Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation |
| title_fullStr | Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation |
| title_full_unstemmed | Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation |
| title_short | Inhibitory Effects of Myricetin on Lipopolysaccharide-Induced Neuroinflammation |
| title_sort | inhibitory effects of myricetin on lipopolysaccharide induced neuroinflammation |
| topic | myricetin inflammation microglia lipopolysaccharide-induced neuroinflammation cytokines |
| url | https://www.mdpi.com/2076-3425/10/1/32 |
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